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Article: Tumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner

TitleTumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2013, v. 8 n. 1, p. e53564 How to Cite?
AbstractPreviously, we have documented that isolated autophagosomes from tumor cells could efficiently cross-prime tumor-reactive naïve T cells and mediate tumor regression in preclinical mouse models. However, the effect of tumor-derived autophagosomes, here we refer as to DRibbles, on B cells has not been studied so far. At present study, we found that DRibbles generated from a murine hepatoma cell line Hep1-6, induced B-cell activation after intravenous injection into mice. B-cell populations were significantly expanded and the production of Hep1-6 tumor-specific antibodies was successfully induced. Moreover, in vitro studies showed that DRibbles could induce more efficient B-cell proliferation and activation, antibody production, and cytokine secretion than whole tumor cell lysates. Notably, we found that B-cell activation required proteins but not DNA in the DRibbles. We further showed that B cells could capture DRibbles and present antigens in the DRibbles to directly induce T cell activation. Furthermore, we found that B-cell activation, antibody production, cytokine secretion and antigen cross-presentation were TLR2-MyD88 pathway dependent. Taken together, the present studies demonstrated that tumor-derived autophagosomes (DRibbles) efficiently induced B cells activation, antibody production, cytokine secretion and antigen cross-presentation mainly depending on their protein component via TLR2/MyD88 dependent manner.
Persistent Identifierhttp://hdl.handle.net/10722/186442
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLi, W-
dc.contributor.authorZhou, M-
dc.contributor.authorRen, H-
dc.contributor.authorHu, HM-
dc.contributor.authorLu, L-
dc.contributor.authorCao, M-
dc.contributor.authorWang, LX-
dc.date.accessioned2013-08-20T12:09:01Z-
dc.date.available2013-08-20T12:09:01Z-
dc.date.issued2013-
dc.identifier.citationPLoS One, 2013, v. 8 n. 1, p. e53564-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/186442-
dc.description.abstractPreviously, we have documented that isolated autophagosomes from tumor cells could efficiently cross-prime tumor-reactive naïve T cells and mediate tumor regression in preclinical mouse models. However, the effect of tumor-derived autophagosomes, here we refer as to DRibbles, on B cells has not been studied so far. At present study, we found that DRibbles generated from a murine hepatoma cell line Hep1-6, induced B-cell activation after intravenous injection into mice. B-cell populations were significantly expanded and the production of Hep1-6 tumor-specific antibodies was successfully induced. Moreover, in vitro studies showed that DRibbles could induce more efficient B-cell proliferation and activation, antibody production, and cytokine secretion than whole tumor cell lysates. Notably, we found that B-cell activation required proteins but not DNA in the DRibbles. We further showed that B cells could capture DRibbles and present antigens in the DRibbles to directly induce T cell activation. Furthermore, we found that B-cell activation, antibody production, cytokine secretion and antigen cross-presentation were TLR2-MyD88 pathway dependent. Taken together, the present studies demonstrated that tumor-derived autophagosomes (DRibbles) efficiently induced B cells activation, antibody production, cytokine secretion and antigen cross-presentation mainly depending on their protein component via TLR2/MyD88 dependent manner.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS One-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleTumor-derived autophagosomes (DRibbles) induce B cell activation in a TLR2-MyD88 dependent manner-
dc.typeArticle-
dc.identifier.emailLu, L: liweilu@hkucc.hku.hk-
dc.identifier.authorityLu, L=rp00477-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0053564-
dc.identifier.pmcidPMC3541185-
dc.identifier.hkuros218878-
dc.identifier.volume8-
dc.identifier.issue1-
dc.identifier.spagee53564-
dc.identifier.epagee53564-
dc.publisher.placeUnited States-

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