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Article: Molecular Characterization of an Atypical IncX3 Plasmid pKPC-NY79 Carrying blaKPC-2 in a Klebsiella pneumoniae

TitleMolecular Characterization of an Atypical IncX3 Plasmid pKPC-NY79 Carrying blaKPC-2 in a Klebsiella pneumoniae
Authors
Issue Date2013
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00284/
Citation
Current Microbiology, 2013, v. 67 n. 4, p. 493-498 How to Cite?
AbstractThe IncX family of plasmids has recently been expanded to include at least four subtypes, IncX1-IncX4. The revised classification provides an opportunity for improving our understanding of the sequence diversity of the IncX plasmids and the resistance genes they carried. We described the complete nucleotide sequence of a novel IncX3 plasmid, pKPC-NY79 (42,447 bp) from a sequence-type 258 Klebsiella pneumoniae strain that was isolated from a patient who was hospitalized in New York, United States. In pKPC-NY79, the plasmid scaffold and genetic load region were highly similar to homologous regions in pIncX-SHV (IncX3, JN247852) and the bla KPC carrying pKpQIL (IncFII k, GU595196), respectively, indicating that it has possibly arisen through recombination of plasmids. The bla KPC-2 gene, as part of a transposon Tn4401a, was found within the genetic load region. The backbone of pKPC-NY79 differs from pIncX-SHV by a deletion involving the gene tandem hns-topB (encoding H-NS protein and topoisomerase III, respectively) and a putative ATPase gene. Unexpectedly, the impact of the hns-topB deletion on host fitness and plasmid stability was found to be small. In conclusion, the findings contribute to a better understanding of the plasmid platforms carrying bla KPC and of variations in the backbone of the IncX3 plasmids. © 2013 Springer Science+Business Media New York.
Persistent Identifierhttp://hdl.handle.net/10722/186079
ISSN
2015 Impact Factor: 1.519
2015 SCImago Journal Rankings: 0.591
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, PL-
dc.contributor.authorCHEUNG, YY-
dc.contributor.authorLo, WU-
dc.contributor.authorLI, Z-
dc.contributor.authorChow, KH-
dc.contributor.authorLin, C-
dc.contributor.authorChan, JFW-
dc.contributor.authorCheng, VCC-
dc.date.accessioned2013-08-20T11:53:31Z-
dc.date.available2013-08-20T11:53:31Z-
dc.date.issued2013-
dc.identifier.citationCurrent Microbiology, 2013, v. 67 n. 4, p. 493-498-
dc.identifier.issn0343-8651-
dc.identifier.urihttp://hdl.handle.net/10722/186079-
dc.description.abstractThe IncX family of plasmids has recently been expanded to include at least four subtypes, IncX1-IncX4. The revised classification provides an opportunity for improving our understanding of the sequence diversity of the IncX plasmids and the resistance genes they carried. We described the complete nucleotide sequence of a novel IncX3 plasmid, pKPC-NY79 (42,447 bp) from a sequence-type 258 Klebsiella pneumoniae strain that was isolated from a patient who was hospitalized in New York, United States. In pKPC-NY79, the plasmid scaffold and genetic load region were highly similar to homologous regions in pIncX-SHV (IncX3, JN247852) and the bla KPC carrying pKpQIL (IncFII k, GU595196), respectively, indicating that it has possibly arisen through recombination of plasmids. The bla KPC-2 gene, as part of a transposon Tn4401a, was found within the genetic load region. The backbone of pKPC-NY79 differs from pIncX-SHV by a deletion involving the gene tandem hns-topB (encoding H-NS protein and topoisomerase III, respectively) and a putative ATPase gene. Unexpectedly, the impact of the hns-topB deletion on host fitness and plasmid stability was found to be small. In conclusion, the findings contribute to a better understanding of the plasmid platforms carrying bla KPC and of variations in the backbone of the IncX3 plasmids. © 2013 Springer Science+Business Media New York.-
dc.languageeng-
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00284/-
dc.relation.ispartofCurrent Microbiology-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleMolecular Characterization of an Atypical IncX3 Plasmid pKPC-NY79 Carrying blaKPC-2 in a Klebsiella pneumoniae-
dc.typeArticle-
dc.identifier.emailHo, PL: plho@hkucc.hku.hk-
dc.identifier.emailLo, WU: stephlo@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.emailLin, C: nicklin@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailCheng, VCC: vcccheng@hkucc.hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityChow, KH=rp00370-
dc.identifier.authorityChan, JFW=rp01736-
dc.description.naturepostprint-
dc.identifier.doi10.1007/s00284-013-0398-2-
dc.identifier.pmid23728748-
dc.identifier.scopuseid_2-s2.0-84883236876-
dc.identifier.hkuros219318-
dc.identifier.volume67-
dc.identifier.issue4-
dc.identifier.spage493-
dc.identifier.epage498-
dc.identifier.isiWOS:000323497900016-
dc.publisher.placeUnited States-

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