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Conference Paper: Towards remyelination therapy with oligodendrocyte precursor derivation from bone marrow stromal cells

TitleTowards remyelination therapy with oligodendrocyte precursor derivation from bone marrow stromal cells
Authors
KeywordsStem cell
Oligodendrocyte
Myelination
Issue Date2012
PublisherSociety for Neuroscience.
Citation
The 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience 2012, 2012, no. 632.06/A6 How to Cite?
AbstractMyelin damage and disorders caused by physical injury or diseases like leukodystrophies result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first cultured as non-adherent spheres until they expressed markers of neural/glial progenitors. The neural/glial progenitors were then maintained in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within 2 weeks and can be expanded in culture for up to 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along neurites, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.
DescriptionPoster Session 632 - Glial Differentiation: no. 632.06/A6
Persistent Identifierhttp://hdl.handle.net/10722/185335

 

DC FieldValueLanguage
dc.contributor.authorTsui, AYPen_US
dc.contributor.authorLi, RSen_US
dc.contributor.authorLo, ACYen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2013-07-15T11:04:02Z-
dc.date.available2013-07-15T11:04:02Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience 2012, 2012, no. 632.06/A6en_US
dc.identifier.urihttp://hdl.handle.net/10722/185335-
dc.descriptionPoster Session 632 - Glial Differentiation: no. 632.06/A6-
dc.description.abstractMyelin damage and disorders caused by physical injury or diseases like leukodystrophies result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first cultured as non-adherent spheres until they expressed markers of neural/glial progenitors. The neural/glial progenitors were then maintained in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within 2 weeks and can be expanded in culture for up to 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along neurites, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2012en_US
dc.rightsAbstracts of the Neuroscience 2012. Copyright © Society for Neuroscience.-
dc.subjectStem cell-
dc.subjectOligodendrocyte-
dc.subjectMyelination-
dc.titleTowards remyelination therapy with oligodendrocyte precursor derivation from bone marrow stromal cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailTsui, AYP: h0694071@hku.hken_US
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityLo, ACY=rp00425en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros216124en_US
dc.identifier.hkuros234964-
dc.publisher.placeUnited States-

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