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Article: A statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza A viruses with elevated polymerase activity

TitleA statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza A viruses with elevated polymerase activity
Authors
Issue Date2013
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1
Citation
Influenza and other respiratory viruses, 2013, v. 7 n. 6, p. 969–978 How to Cite?
AbstractObjectives: Reassortment of influenza A viruses can give rise to viral ribonucleoproteins (vRNPs) with elevated polymerase activity and the previous three pandemic influenza viruses contained reassorted vRNPs of different origins. These suggest that reassorted vRNP may be one of the factors leading to a pandemic virus. In this study, we reconstituted chimeric vRNPs with three different viral strains isolated from avian, human and swine hosts. We applied a statistical strategy to identify the effect that the origin of a single vRNP protein subunit or the interactions between these subunits on polymerase activity. Design: Eighty one chimeric vRNPs were reconstituted in 293T cells at different temperatures. Polymerase activity was determined by luciferase reporter assay and the results were analysed by multiway anova and other statistical methods. Results: It was found that PB2, PB1, NP, PB2-PB1 interaction, PB2-PA interaction and PB1-NP interaction had significant effect on polymerase activity at 37°C and several single subunits and interactions were identified to lead to elevation of polymerase activity. Furthermore, we studied 27 out of these 81 different chimieric vRNPs in different combinations via fractional factorial design approach. Our results suggested that the approach can identify the major single subunit or interaction factors that affect the polymerase activity without the need to experimentally reproduce all possible vRNP combinations. Conclusions: Statistical approach and fractional factorial design are useful to identify the major single subunit or interaction factors that can modulate viral polymerase activity. © 2013 John Wiley & Sons Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/185314
ISSN
2015 Impact Factor: 2.378
2015 SCImago Journal Rankings: 1.570
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChin, WHen_US
dc.contributor.authorGreenbaum, BDen_US
dc.contributor.authorLi, OTWen_US
dc.contributor.authorWebby, RJen_US
dc.contributor.authorPoon, LLMen_US
dc.date.accessioned2013-07-15T11:00:08Z-
dc.date.available2013-07-15T11:00:08Z-
dc.date.issued2013en_US
dc.identifier.citationInfluenza and other respiratory viruses, 2013, v. 7 n. 6, p. 969–978en_US
dc.identifier.issn1750-2640en_US
dc.identifier.urihttp://hdl.handle.net/10722/185314-
dc.description.abstractObjectives: Reassortment of influenza A viruses can give rise to viral ribonucleoproteins (vRNPs) with elevated polymerase activity and the previous three pandemic influenza viruses contained reassorted vRNPs of different origins. These suggest that reassorted vRNP may be one of the factors leading to a pandemic virus. In this study, we reconstituted chimeric vRNPs with three different viral strains isolated from avian, human and swine hosts. We applied a statistical strategy to identify the effect that the origin of a single vRNP protein subunit or the interactions between these subunits on polymerase activity. Design: Eighty one chimeric vRNPs were reconstituted in 293T cells at different temperatures. Polymerase activity was determined by luciferase reporter assay and the results were analysed by multiway anova and other statistical methods. Results: It was found that PB2, PB1, NP, PB2-PB1 interaction, PB2-PA interaction and PB1-NP interaction had significant effect on polymerase activity at 37°C and several single subunits and interactions were identified to lead to elevation of polymerase activity. Furthermore, we studied 27 out of these 81 different chimieric vRNPs in different combinations via fractional factorial design approach. Our results suggested that the approach can identify the major single subunit or interaction factors that affect the polymerase activity without the need to experimentally reproduce all possible vRNP combinations. Conclusions: Statistical approach and fractional factorial design are useful to identify the major single subunit or interaction factors that can modulate viral polymerase activity. © 2013 John Wiley & Sons Ltd.-
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1en_US
dc.relation.ispartofInfluenza and other respiratory virusesen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleA statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza A viruses with elevated polymerase activityen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1750-2640&volume=&spage=&epage=&date=2013&atitle=A+statistical+strategy+to+identify+recombinant+viral+ribonucleoprotein+of+avian,+human,+and+swine+influenza+A+viruses+with+elevated+polymerase+activityen_US
dc.identifier.emailChin, WH: alexchin@hku.hken_US
dc.identifier.emailLi, OTW: litwo@hku.hken_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/irv.12117-
dc.identifier.pmid23634764-
dc.identifier.scopuseid_2-s2.0-84887184780-
dc.identifier.hkuros216130en_US
dc.identifier.isiWOS:000331001400014-

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