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Conference Paper: Serotonergic enhancement of synaptic transmission in rat medial vestibular nucleus

TitleSerotonergic enhancement of synaptic transmission in rat medial vestibular nucleus
Authors
KeywordsSerotonin
Vestibular
Patch clamp
Issue Date2012
PublisherSociety for Neuroscience.
Citation
The 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience, 2012, no. 430.14/B50 How to Cite?
AbstractTo address the role of serotonergic projections to the medial vestibular nucleus (MV), whole-cell patch-clamp method was used to investigate serotonergic modulation of spontaneous synaptic transmission within the MV of young rats. Perfusion of serotonin (5-hydroxytryptamine, 5-HT) to brainstem slices greatly facilitated spontaneous inhibitory postsynaptic currents (sIPSCs) in a subgroup of MV neurons. That this enhancement effect of 5-HT on sIPSCs was abolished with tetrodotoxin indicated that 5-HT acted on its receptor that expressed in the presynaptic neurons. Using GABAA or glycine receptor antagonist, we further identified that both receptors took part in the enhancement effect of 5-HT on sIPSCs. This facilitatory effect could be mimicked with the administration of 5-HT2 receptor agonist but was blocked with 5-HT2A receptor antagonist. Similarly, 5-HT significantly increased both the amplitude and frequency of spontaneous postsynaptic currents (sEPSCs) in the MV circuitry. This 5-HT-induced enhancement of sEPSCs also depended on action potential. Such an enhancement of sEPSCs was abolished in the presence of CNQX. Further, 5-HT2 receptor agonist could mimic the 5-HT-induced facilitatory effect whereas 5-HT2A receptor antagonist blocked the effect. Our results therefore indicate that within the MV, 5-HT2A receptors expressed on presynaptic neurons function to facilitate both inhibitory and excitatory synaptic transmission.
DescriptionPoster Session 430 - Serotonin Receptors: no. 430.14/B50
Persistent Identifierhttp://hdl.handle.net/10722/185061

 

DC FieldValueLanguage
dc.contributor.authorHan, Len_US
dc.contributor.authorLai, SKen_US
dc.contributor.authorLai, CHen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2013-07-15T10:26:59Z-
dc.date.available2013-07-15T10:26:59Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience, 2012, no. 430.14/B50en_US
dc.identifier.urihttp://hdl.handle.net/10722/185061-
dc.descriptionPoster Session 430 - Serotonin Receptors: no. 430.14/B50-
dc.description.abstractTo address the role of serotonergic projections to the medial vestibular nucleus (MV), whole-cell patch-clamp method was used to investigate serotonergic modulation of spontaneous synaptic transmission within the MV of young rats. Perfusion of serotonin (5-hydroxytryptamine, 5-HT) to brainstem slices greatly facilitated spontaneous inhibitory postsynaptic currents (sIPSCs) in a subgroup of MV neurons. That this enhancement effect of 5-HT on sIPSCs was abolished with tetrodotoxin indicated that 5-HT acted on its receptor that expressed in the presynaptic neurons. Using GABAA or glycine receptor antagonist, we further identified that both receptors took part in the enhancement effect of 5-HT on sIPSCs. This facilitatory effect could be mimicked with the administration of 5-HT2 receptor agonist but was blocked with 5-HT2A receptor antagonist. Similarly, 5-HT significantly increased both the amplitude and frequency of spontaneous postsynaptic currents (sEPSCs) in the MV circuitry. This 5-HT-induced enhancement of sEPSCs also depended on action potential. Such an enhancement of sEPSCs was abolished in the presence of CNQX. Further, 5-HT2 receptor agonist could mimic the 5-HT-induced facilitatory effect whereas 5-HT2A receptor antagonist blocked the effect. Our results therefore indicate that within the MV, 5-HT2A receptors expressed on presynaptic neurons function to facilitate both inhibitory and excitatory synaptic transmission.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofAbstracts of the Neuroscience 2012en_US
dc.rightsAbstracts of the Neuroscience 2012. Copyright © Society for Neuroscience.-
dc.subjectSerotonin-
dc.subjectVestibular-
dc.subjectPatch clamp-
dc.titleSerotonergic enhancement of synaptic transmission in rat medial vestibular nucleusen_US
dc.typeConference_Paperen_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailHan, L: rahanlei@hku.hken_US
dc.identifier.emailLai, CH: chlaib@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.authorityLai, CH=rp00396en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros216086en_US
dc.publisher.placeUnited States-

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