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Conference Paper: Notch signaling mediates the switch to fate commitment of bone marrow-derived Schwann cells

TitleNotch signaling mediates the switch to fate commitment of bone marrow-derived Schwann cells
Authors
KeywordsBone marrow stromal cells
Schwann cell-like cells
Cell fate commitment
Issue Date2012
PublisherSociety for Neuroscience.
Citation
The 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience, 2012, no. 131.11/A11 How to Cite?
AbstractOur strategy of deriving Schwann cells from bone marrow stromal cells (Shea et al., 2010) exploits dorsal root ganglia (DRG) neurons to provide Schwann cell-like cells with juxtacrine signals for commitment to the Schwann cell fate. In our search for the signals, immunocytochemical analysis found DLL-1 and Jagged-1, localized on the surface of the DRG neurons whereas the receptor, Notch-1, on bone marrow-derived Schwann cell-like cells. In cocultures with DRG neurons, Schwann cell-like cells were identifiable with nuclear localization of the Notch intracellular domain (NICD). Concomitantly, progressive increase in ErbB2/B3 expression was revealed by immunocytochemistry and Western blotting. As cells achieved commitment to the Schwann cell fate, nuclear NICD was found to return to basal level. Altogether, Notch-ligand interaction between Schwann cell-like cells and DRG neurons in contact are therefore implicated in signalling events that accomplish commitment to the Schwann cell fate in vitro.
DescriptionPoster Session 131 - Neuron-Glia Interaction: no. 131.11/A11
Persistent Identifierhttp://hdl.handle.net/10722/184910

 

DC FieldValueLanguage
dc.contributor.authorShum, DKYen_US
dc.contributor.authorTai, EWYen_US
dc.contributor.authorShea, GKHen_US
dc.contributor.authorTsui, AYPen_US
dc.contributor.authorLeung, KHYen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2013-07-15T10:18:20Z-
dc.date.available2013-07-15T10:18:20Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting of the Society for Neuroscience (SfN), New Orleans, LA., 13-17 October 2012. In Abstracts of the Neuroscience, 2012, no. 131.11/A11en_US
dc.identifier.urihttp://hdl.handle.net/10722/184910-
dc.descriptionPoster Session 131 - Neuron-Glia Interaction: no. 131.11/A11-
dc.description.abstractOur strategy of deriving Schwann cells from bone marrow stromal cells (Shea et al., 2010) exploits dorsal root ganglia (DRG) neurons to provide Schwann cell-like cells with juxtacrine signals for commitment to the Schwann cell fate. In our search for the signals, immunocytochemical analysis found DLL-1 and Jagged-1, localized on the surface of the DRG neurons whereas the receptor, Notch-1, on bone marrow-derived Schwann cell-like cells. In cocultures with DRG neurons, Schwann cell-like cells were identifiable with nuclear localization of the Notch intracellular domain (NICD). Concomitantly, progressive increase in ErbB2/B3 expression was revealed by immunocytochemistry and Western blotting. As cells achieved commitment to the Schwann cell fate, nuclear NICD was found to return to basal level. Altogether, Notch-ligand interaction between Schwann cell-like cells and DRG neurons in contact are therefore implicated in signalling events that accomplish commitment to the Schwann cell fate in vitro.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofAbstracts of the Neuroscience 2012en_US
dc.rightsAbstracts of the Neuroscience 2012. Copyright © Society for Neuroscience.-
dc.subjectBone marrow stromal cells-
dc.subjectSchwann cell-like cells-
dc.subjectCell fate commitment-
dc.titleNotch signaling mediates the switch to fate commitment of bone marrow-derived Schwann cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.emailTai, EWY: evietai@hku.hken_US
dc.identifier.emailShea, GKH: grahams@hku.hk-
dc.identifier.emailTsui, AYP: h0694071@hku.hk-
dc.identifier.emailLeung, KHY: u3001336@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros216082en_US
dc.publisher.placeUnited States-

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