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Conference Paper: Effect of Fluoride-modified Titanium Surface on Irradiated Osteoblast Adhesion

TitleEffect of Fluoride-modified Titanium Surface on Irradiated Osteoblast Adhesion
Authors
KeywordsCell culture
Fluoride
Implants and Osteoblasts/osteoclasts
Issue Date2012
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 6th International Association for Dental Research Pan-European Region Meeting (IADR/PER), Helsinki, Finland, 12-15 September 2012. In Journal of Dental Research, 2012, v. 91 n. Special Issue C: abstract no. 152 How to Cite?
AbstractObjectives: To investigate the effect of fluoride-modified titanium surface on early adhesion of irradiated osteoblasts. Methods: Human osteoblast-like cell lines (SaOS-2) in density of 2×104cell/mL were radiated. Cells received a single dose of 0Gy, 2Gy, 4Gy, 6Gy, 8Gy, 10Gy radiation respectively using a 10 MV photon. After irradiation, 40µl cell suspension was seeded onto either titanium dioxide blasted discs with (test group) or without (control group) fluoride-modified surface treatment respectively. The discs were then cultured under standard conditions for 24 hours. Cell number was assessed by DAPI staining and fluorescence microscope observation; cytoskeletal changes were analyzed using phalloidine for f-actin staining and cell area was measured using fluorescence microscope. The mean values between the two groups were compared by independent t-test and differences among different radiation doses were compared by one-way ANOVA. Differences were considered significant at P < .05. Results: There was a significant reduction in the mean number of osteoblasts with increasing irradiation dosage in both groups. The mean number of osteoblasts in test group was significantly less than the control group at 8Gy and 10Gy. There was a significant increase in the mean cell area with increasing irradiation dosage in the control group while in the test group, the mean cell area of osteoblasts at 2Gy and 4Gy was significantly less than other dosages. The mean cell area of osteoblasts in the control group was significantly larger than the test group at 4Gy and 6Gy. Conclusions: This in vitro study showed that irradiation compromised the early cell adhesion of osteoblasts to titanium discs. The fluoride surface treatment on titanium discs affects the behaviour of irradiated osteoblasts and that might partly be due to the more hydrophobic surface with increased roughness.
DescriptionPoster Presentation
Session 23: Implantology
Persistent Identifierhttp://hdl.handle.net/10722/184868
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_US
dc.contributor.authorPow, EHNen_US
dc.contributor.authorZheng, Len_US
dc.contributor.authorMa, Len_US
dc.contributor.authorKwong, DLWen_US
dc.contributor.authorCheung, LKen_US
dc.date.accessioned2013-07-15T10:15:42Z-
dc.date.available2013-07-15T10:15:42Z-
dc.date.issued2012en_US
dc.identifier.citationThe 6th International Association for Dental Research Pan-European Region Meeting (IADR/PER), Helsinki, Finland, 12-15 September 2012. In Journal of Dental Research, 2012, v. 91 n. Special Issue C: abstract no. 152en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttp://hdl.handle.net/10722/184868-
dc.descriptionPoster Presentation-
dc.descriptionSession 23: Implantology-
dc.description.abstractObjectives: To investigate the effect of fluoride-modified titanium surface on early adhesion of irradiated osteoblasts. Methods: Human osteoblast-like cell lines (SaOS-2) in density of 2×104cell/mL were radiated. Cells received a single dose of 0Gy, 2Gy, 4Gy, 6Gy, 8Gy, 10Gy radiation respectively using a 10 MV photon. After irradiation, 40µl cell suspension was seeded onto either titanium dioxide blasted discs with (test group) or without (control group) fluoride-modified surface treatment respectively. The discs were then cultured under standard conditions for 24 hours. Cell number was assessed by DAPI staining and fluorescence microscope observation; cytoskeletal changes were analyzed using phalloidine for f-actin staining and cell area was measured using fluorescence microscope. The mean values between the two groups were compared by independent t-test and differences among different radiation doses were compared by one-way ANOVA. Differences were considered significant at P < .05. Results: There was a significant reduction in the mean number of osteoblasts with increasing irradiation dosage in both groups. The mean number of osteoblasts in test group was significantly less than the control group at 8Gy and 10Gy. There was a significant increase in the mean cell area with increasing irradiation dosage in the control group while in the test group, the mean cell area of osteoblasts at 2Gy and 4Gy was significantly less than other dosages. The mean cell area of osteoblasts in the control group was significantly larger than the test group at 4Gy and 6Gy. Conclusions: This in vitro study showed that irradiation compromised the early cell adhesion of osteoblasts to titanium discs. The fluoride surface treatment on titanium discs affects the behaviour of irradiated osteoblasts and that might partly be due to the more hydrophobic surface with increased roughness.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925en_US
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..en_US
dc.subjectCell culture-
dc.subjectFluoride-
dc.subjectImplants and Osteoblasts/osteoclasts-
dc.titleEffect of Fluoride-modified Titanium Surface on Irradiated Osteoblast Adhesionen_US
dc.typeConference_Paperen_US
dc.identifier.emailPow, EHN: ehnpow@hku.hken_US
dc.identifier.emailZheng, L: lwzheng@hku.hken_US
dc.identifier.emailMa, L: lima@hku.hken_US
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_US
dc.identifier.emailCheung, LK: lkcheung@hkucc.hku.hken_US
dc.identifier.authorityPow, EHN=rp00030en_US
dc.identifier.authorityZheng, L=rp01411en_US
dc.identifier.authorityKwong, DLW=rp00414en_US
dc.identifier.authorityCheung, LK=rp00013en_US
dc.identifier.hkuros215128en_US
dc.identifier.volume91en_US
dc.identifier.issueSpecial Issue C: abstract no. 152en_US
dc.publisher.placeUnited States-

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