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Article: cDNA synthesis and molecular cloning of simian retrovirus type 1

TitlecDNA synthesis and molecular cloning of simian retrovirus type 1
猴D型逆轉病毒Ⅰ型cDNA合成及其分子克隆的建立
Authors
KeywordsSRV-1
cDNA合成
分子克隆
Southern雜交
光敏生物素探針
cDNA synthesis
Molecular cloning
Southern hybridization
Photobiotin probe
Issue Date1991
Publisher中國實驗動物學雜誌編輯部.
Citation
Chinese Journal of Laboratory Animal Science, 1991, v. 1 n. 2, p. 88-92 How to Cite?
中國實驗動物學雜誌, 1991, v. 1 n. 2, p. 88-92 How to Cite?
Abstract利用隨機引物法反轉錄合成SRV-1 ss-cDNA和ds—cDNA.將ds—cDNA平端連接到質粒pUC19中,轉化E.Coli.DH5α菌.氨芐青霉素、X-gal培基和菌落雜交篩選,克隆陽性重組率為20.7%.EcoRⅠ、BamHⅠ雙酶切分析,12.7%的插入片段大于500bp。Southern雜交進一步確證,所選三株重組質粒(pSRV-127、pSRV-156和pSRV-169)的探針用插入片段(0.9kb、1.0kb和1.4kb),是SRV-1 cDNA片段.光敏生物素標記純化pSRV-169DNA和pSRV-156cDNA片段制備探針,前者用于檢測SRV-1病毒RNA,后者用于檢測純化病毒顆粒。堿性磷酸酶系統染色,靈敏度分別為18pg和4×105vp.
Persistent Identifierhttp://hdl.handle.net/10722/184311
ISSN

 

DC FieldValueLanguage
dc.contributor.authorChen, Z-
dc.date.accessioned2013-07-08T09:49:28Z-
dc.date.available2013-07-08T09:49:28Z-
dc.date.issued1991-
dc.identifier.citationChinese Journal of Laboratory Animal Science, 1991, v. 1 n. 2, p. 88-92-
dc.identifier.citation中國實驗動物學雜誌, 1991, v. 1 n. 2, p. 88-92-
dc.identifier.issn1002-1485-
dc.identifier.urihttp://hdl.handle.net/10722/184311-
dc.description.abstract利用隨機引物法反轉錄合成SRV-1 ss-cDNA和ds—cDNA.將ds—cDNA平端連接到質粒pUC19中,轉化E.Coli.DH5α菌.氨芐青霉素、X-gal培基和菌落雜交篩選,克隆陽性重組率為20.7%.EcoRⅠ、BamHⅠ雙酶切分析,12.7%的插入片段大于500bp。Southern雜交進一步確證,所選三株重組質粒(pSRV-127、pSRV-156和pSRV-169)的探針用插入片段(0.9kb、1.0kb和1.4kb),是SRV-1 cDNA片段.光敏生物素標記純化pSRV-169DNA和pSRV-156cDNA片段制備探針,前者用于檢測SRV-1病毒RNA,后者用于檢測純化病毒顆粒。堿性磷酸酶系統染色,靈敏度分別為18pg和4×105vp.-
dc.languagechi-
dc.languageeng-
dc.publisher中國實驗動物學雜誌編輯部.-
dc.relation.ispartofChinese Journal of Laboratory Animal Science-
dc.relation.ispartof中國實驗動物學雜誌-
dc.subjectSRV-1-
dc.subjectcDNA合成-
dc.subject分子克隆-
dc.subjectSouthern雜交-
dc.subject光敏生物素探針-
dc.subjectcDNA synthesis-
dc.subjectMolecular cloning-
dc.subjectSouthern hybridization-
dc.subjectPhotobiotin probe-
dc.titlecDNA synthesis and molecular cloning of simian retrovirus type 1en_US
dc.title猴D型逆轉病毒Ⅰ型cDNA合成及其分子克隆的建立-
dc.typeArticleen_US
dc.identifier.emailChen, Z: zchenai@hkucc.hku.hk-
dc.identifier.volume1-
dc.identifier.issue2-
dc.identifier.spage88-
dc.identifier.epage92-
dc.publisher.place北京-

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