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Article: Comparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell line

TitleComparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell line
Authors
KeywordsCell Line Models
Hepatoblastoma
Hepatocellular Carcinoma
Proteome
Two-Dimensional Polyacrylamide Gel Electrophoresis
Issue Date2004
Citation
Clinical Proteomics, 2004, v. 1 n. 3-4, p. 313-331 How to Cite?
AbstractHepatocellular carcinoma (HCC) and hepatoblastoma (HB) are malignancies of the liver with different etiologies, but the HB cell line HepG2 has been frequently used in various studies of HCC. In this study, we compare the protein expression patterns between HepG2 cells and three HCC cell lines, HKCI-2, HKCI-3, and HKCI-4, respectively. The cell lysates of individual cell lines were separated by two-dimensional polyacrylamide gel electrophoresis. The protein spots in the gel images were quantified and compared by image analysis software. The differentially expressing proteins were then identified by tryptic peptide mass fingerprinting. Compared with the HepG2 cells, the normalized quantities of 49 and 58 protein spots were found to be at least twofold higher and twofold lower, respectively, in all three HCC cell lines. The differentially expressed proteins can be grouped into structural proteins (annexins, transgelin, laminin receptor), stress-induced proteins (HSP27, 60, and 70), enzymes (aldehyde dehydrogenase, pyruvate kinase, α-enolase, etc.), and transcription factors (far upstream element binding protein 2, GTP-binding nuclear protein RAN). Some of these proteins play important roles in regulating homeostasis, drug resistance, apoptosis, cell differentiation, cell growth, and metastasis. In conclusion, our proteomic data indicate that there are considerable differences in the protein expression patterns between HepG2 cells and the HCC cells, suggesting differences m cellular properties. Hence, HepG2 may not be a good cell line model for studying HCC. Copyright © Humana Press Inc. All rights of any nature whatsoever are reserved.
Persistent Identifierhttp://hdl.handle.net/10722/184288
ISSN
2015 Impact Factor: 3.476
2015 SCImago Journal Rankings: 1.210
References

 

DC FieldValueLanguage
dc.contributor.authorPang, RTKen_US
dc.contributor.authorPoon, TCWen_US
dc.contributor.authorWong, Nen_US
dc.contributor.authorLai, PBSen_US
dc.contributor.authorWong, NLYen_US
dc.contributor.authorChan, CMLen_US
dc.contributor.authorYu, JWSen_US
dc.contributor.authorChan, ATCen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2013-07-04T06:10:49Z-
dc.date.available2013-07-04T06:10:49Z-
dc.date.issued2004en_US
dc.identifier.citationClinical Proteomics, 2004, v. 1 n. 3-4, p. 313-331en_US
dc.identifier.issn1542-6416en_US
dc.identifier.urihttp://hdl.handle.net/10722/184288-
dc.description.abstractHepatocellular carcinoma (HCC) and hepatoblastoma (HB) are malignancies of the liver with different etiologies, but the HB cell line HepG2 has been frequently used in various studies of HCC. In this study, we compare the protein expression patterns between HepG2 cells and three HCC cell lines, HKCI-2, HKCI-3, and HKCI-4, respectively. The cell lysates of individual cell lines were separated by two-dimensional polyacrylamide gel electrophoresis. The protein spots in the gel images were quantified and compared by image analysis software. The differentially expressing proteins were then identified by tryptic peptide mass fingerprinting. Compared with the HepG2 cells, the normalized quantities of 49 and 58 protein spots were found to be at least twofold higher and twofold lower, respectively, in all three HCC cell lines. The differentially expressed proteins can be grouped into structural proteins (annexins, transgelin, laminin receptor), stress-induced proteins (HSP27, 60, and 70), enzymes (aldehyde dehydrogenase, pyruvate kinase, α-enolase, etc.), and transcription factors (far upstream element binding protein 2, GTP-binding nuclear protein RAN). Some of these proteins play important roles in regulating homeostasis, drug resistance, apoptosis, cell differentiation, cell growth, and metastasis. In conclusion, our proteomic data indicate that there are considerable differences in the protein expression patterns between HepG2 cells and the HCC cells, suggesting differences m cellular properties. Hence, HepG2 may not be a good cell line model for studying HCC. Copyright © Humana Press Inc. All rights of any nature whatsoever are reserved.en_US
dc.languageengen_US
dc.relation.ispartofClinical Proteomicsen_US
dc.subjectCell Line Modelsen_US
dc.subjectHepatoblastomaen_US
dc.subjectHepatocellular Carcinomaen_US
dc.subjectProteomeen_US
dc.subjectTwo-Dimensional Polyacrylamide Gel Electrophoresisen_US
dc.titleComparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell lineen_US
dc.typeArticleen_US
dc.identifier.emailPang, RTK: rtkpang@hku.hken_US
dc.identifier.authorityPang, RTK=rp01761en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1385/CP:1:3-4:313en_US
dc.identifier.scopuseid_2-s2.0-24644472994en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24644472994&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1en_US
dc.identifier.issue3-4en_US
dc.identifier.spage313en_US
dc.identifier.epage331en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPang, RTK=7004376636en_US
dc.identifier.scopusauthoridPoon, TCW=7006151710en_US
dc.identifier.scopusauthoridWong, N=7202836653en_US
dc.identifier.scopusauthoridLai, PBS=7202946421en_US
dc.identifier.scopusauthoridWong, NLY=25937585300en_US
dc.identifier.scopusauthoridChan, CML=36096478600en_US
dc.identifier.scopusauthoridYu, JWS=7405527235en_US
dc.identifier.scopusauthoridChan, ATC=13404833700en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US

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