File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

postgraduate thesis: Exploring microbial community structures and functions of activated sludge by high-throughput sequencing

TitleExploring microbial community structures and functions of activated sludge by high-throughput sequencing
Authors
Issue Date2012
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Ye, L. [叶林]. (2012). Exploring microbial community structures and functions of activated sludge by high-throughput sequencing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807964
AbstractTo investigate the diversities and abundances of nitrifiers and to apply the highthroughput sequencing technologies to analyze the overall microbial community structures and functions in the wastewater treatment bioreactors were the major objectives of this study. Specifically, this study was conducted: (1) to investigate the diversities and abundances of AOA, AOB and NOB in bioreactors, (2) to explore the bacterial communities in bioreactors using 454 pyrosequencing, and (3) to analyze the metagenomes of activated sludge using Illumina sequencing. A lab-scale nitrification bioreactor was operated for 342 days under low DO (0.15~0.5 mg/L) and high nitrogen loading (0.26~0.52 kg-N/(m3d)). T-RFLP and cloning analysis showed there were only one dominant AOA, AOB and NOB species in the bioreactor, respectively. The amoA gene of the dominant AOA had a similarity of 89.3% with the isolated AOA species Nitrosopumilus maritimus SCM1. The AOB species detected in the bioreactor belonged to Nitrosomonas genus. The abundance of AOB was more than 40 times larger than that of AOA. The percentage of NOB in total bacteria increased from not detectable to 30% when DO changed from 0.15 to 0.5 mg/L. Compared with traditional methods, pyrosequencing analysis of the bacteria in this bioreactor provided unprecedented information. 494 bacterial OTUs was obtained at 3% distance cutoff. Furthermore, 454 pyrosequencing was applied to investigate the bacterial communities of activated sludge samples from 14 WWTPs of Asia (mainland China, Hong Kong, and Singapore) and North America (Canada and the United States). The results revealed huge amounts of OTUs in activated sludge, i.e. 1183~3567 OTUs in one sludge sample at 3% distance cutoff. Clear geographical differences among these samples were observed. The AOB amoA genes in different WWTPs were found quite diverse while the 16S rRNA genes were relatively conserved. To explore microbial community structures and functions in the abovementioned labscale bioreactor and a full-scale bioreactor, over six gigabases of metagenomic sequence data and 150,000 paired-end reads of PCR amplicons were generated from the activated sludge in the two bioreactors on Illumina HiSeq2000 platform. Three kinds of sequences (16S rRNA amplicons, 16S rRNA gene tags and predicted genes) were used to conduct taxonomic assignment and their applicabilities and reliabilities were compared. Specially, based on 16S rRNA and amoA gene sequences, AOB were found more abundant than AOA in the two bioreactors. Furthermore, the analysis of the metabolic profiles and pathways indicated that the overall pathways in the two bioreactors were quite similar. However, the abundances of some specific genes in the two bioreactors were different. In addition, 454 pyrosequencing was also used to detect potentially pathogenic bacteria in environmental samples. It was found most abundant potentially pathogenic bacteria in the WWTPs were affiliated with Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Overall, the percentage of the sequences closely related to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a Java application (BAND) was developed for graphical visualization of microbial abundance data.
DegreeDoctor of Philosophy
SubjectSequence alignment (Bioinformatics)
Microbial genomics - Data processing.
Dept/ProgramCivil Engineering
Persistent Identifierhttp://hdl.handle.net/10722/184237

 

DC FieldValueLanguage
dc.contributor.authorYe, Lin-
dc.contributor.author叶林-
dc.date.accessioned2013-06-29T15:45:40Z-
dc.date.available2013-06-29T15:45:40Z-
dc.date.issued2012-
dc.identifier.citationYe, L. [叶林]. (2012). Exploring microbial community structures and functions of activated sludge by high-throughput sequencing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4807964-
dc.identifier.urihttp://hdl.handle.net/10722/184237-
dc.description.abstractTo investigate the diversities and abundances of nitrifiers and to apply the highthroughput sequencing technologies to analyze the overall microbial community structures and functions in the wastewater treatment bioreactors were the major objectives of this study. Specifically, this study was conducted: (1) to investigate the diversities and abundances of AOA, AOB and NOB in bioreactors, (2) to explore the bacterial communities in bioreactors using 454 pyrosequencing, and (3) to analyze the metagenomes of activated sludge using Illumina sequencing. A lab-scale nitrification bioreactor was operated for 342 days under low DO (0.15~0.5 mg/L) and high nitrogen loading (0.26~0.52 kg-N/(m3d)). T-RFLP and cloning analysis showed there were only one dominant AOA, AOB and NOB species in the bioreactor, respectively. The amoA gene of the dominant AOA had a similarity of 89.3% with the isolated AOA species Nitrosopumilus maritimus SCM1. The AOB species detected in the bioreactor belonged to Nitrosomonas genus. The abundance of AOB was more than 40 times larger than that of AOA. The percentage of NOB in total bacteria increased from not detectable to 30% when DO changed from 0.15 to 0.5 mg/L. Compared with traditional methods, pyrosequencing analysis of the bacteria in this bioreactor provided unprecedented information. 494 bacterial OTUs was obtained at 3% distance cutoff. Furthermore, 454 pyrosequencing was applied to investigate the bacterial communities of activated sludge samples from 14 WWTPs of Asia (mainland China, Hong Kong, and Singapore) and North America (Canada and the United States). The results revealed huge amounts of OTUs in activated sludge, i.e. 1183~3567 OTUs in one sludge sample at 3% distance cutoff. Clear geographical differences among these samples were observed. The AOB amoA genes in different WWTPs were found quite diverse while the 16S rRNA genes were relatively conserved. To explore microbial community structures and functions in the abovementioned labscale bioreactor and a full-scale bioreactor, over six gigabases of metagenomic sequence data and 150,000 paired-end reads of PCR amplicons were generated from the activated sludge in the two bioreactors on Illumina HiSeq2000 platform. Three kinds of sequences (16S rRNA amplicons, 16S rRNA gene tags and predicted genes) were used to conduct taxonomic assignment and their applicabilities and reliabilities were compared. Specially, based on 16S rRNA and amoA gene sequences, AOB were found more abundant than AOA in the two bioreactors. Furthermore, the analysis of the metabolic profiles and pathways indicated that the overall pathways in the two bioreactors were quite similar. However, the abundances of some specific genes in the two bioreactors were different. In addition, 454 pyrosequencing was also used to detect potentially pathogenic bacteria in environmental samples. It was found most abundant potentially pathogenic bacteria in the WWTPs were affiliated with Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Overall, the percentage of the sequences closely related to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a Java application (BAND) was developed for graphical visualization of microbial abundance data.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.source.urihttp://hub.hku.hk/bib/B48079649-
dc.subject.lcshSequence alignment (Bioinformatics)-
dc.subject.lcshMicrobial genomics - Data processing.-
dc.titleExploring microbial community structures and functions of activated sludge by high-throughput sequencing-
dc.typePG_Thesis-
dc.identifier.hkulb4807964-
dc.description.thesisnameDoctor of Philosophy-
dc.description.thesislevelDoctoral-
dc.description.thesisdisciplineCivil Engineering-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.5353/th_b4807964-
dc.date.hkucongregation2012-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats