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Article: Effect of reserpine on fertilizing capacity of human spermatozoa

TitleEffect of reserpine on fertilizing capacity of human spermatozoa
Authors
Issue Date1984
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraception
Citation
Contraception, 1984, v. 30 n. 4, p. 363-369 How to Cite?
AbstractThe effect of reserpine at various concentrations (2 x 10-6, 2 x 10-7, 2 x 10-8, 2 x 10-9 and 2 x 10-10M) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations of reserpine for 5 hr before insemination of the zona-free hamster ova. The spermatozoa penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Reserpine, at all the concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (p < 0.05). The percent mobility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated spermatozoa (p > 0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide an additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. | The effect of reserpine at various concentrations (2x10-6, 2x10-7 2x10-8, 2x10-9, and 2x10-10 m) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa was collected from presumably fertile men and washed in BWW medium and incubated with different concentrations of reserpine for 5 hours prior to insemination of the zona-free hamster ova. The spermatozoal penetration of the zona-free hamster ova was scored 6 hours later and results were analyzed statistically. Reserpine, at all concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (P0.05). The percent motility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated sprmatozoa (P0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. author's modified
Persistent Identifierhttp://hdl.handle.net/10722/184163
ISSN
2015 Impact Factor: 2.788
2015 SCImago Journal Rankings: 1.557
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, SYWen_US
dc.contributor.authorTang, LCHen_US
dc.date.accessioned2013-06-25T03:00:54Z-
dc.date.available2013-06-25T03:00:54Z-
dc.date.issued1984en_US
dc.identifier.citationContraception, 1984, v. 30 n. 4, p. 363-369en_US
dc.identifier.issn0010-7824en_US
dc.identifier.urihttp://hdl.handle.net/10722/184163-
dc.description.abstractThe effect of reserpine at various concentrations (2 x 10-6, 2 x 10-7, 2 x 10-8, 2 x 10-9 and 2 x 10-10M) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations of reserpine for 5 hr before insemination of the zona-free hamster ova. The spermatozoa penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Reserpine, at all the concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (p < 0.05). The percent mobility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated spermatozoa (p > 0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide an additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. | The effect of reserpine at various concentrations (2x10-6, 2x10-7 2x10-8, 2x10-9, and 2x10-10 m) on the in vitro fertilizing capacity of human spermatozoa was studied. Spermatozoa was collected from presumably fertile men and washed in BWW medium and incubated with different concentrations of reserpine for 5 hours prior to insemination of the zona-free hamster ova. The spermatozoal penetration of the zona-free hamster ova was scored 6 hours later and results were analyzed statistically. Reserpine, at all concentrations tested, caused a significant dose-dependent decrease in the penetration of the denuded hamster ova in comparison to the control (P0.05). The percent motility of spermatozoa decreased as a function of time during the preincubation period to initiate spermatozoal capacitation but there were no significant differences in the values between the control and the reserpine-treated sprmatozoa (P0.05). These findings indicate that reserpine can affect the fertilizing capacity of human spermatozoa in vitro and provide additional evidence to suggest the prospective use of reserpine as a vaginal contraceptive. author's modifieden_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraceptionen_US
dc.relation.ispartofContraceptionen_US
dc.subject.meshAdulten_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilization - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshReserpine - Pharmacologyen_US
dc.subject.meshSperm-Ovum Interactions - Drug Effectsen_US
dc.subject.meshSpermatozoa - Drug Effectsen_US
dc.titleEffect of reserpine on fertilizing capacity of human spermatozoaen_US
dc.typeArticleen_US
dc.identifier.emailTang, LCH: lchtang@hku.hken_US
dc.identifier.authorityTang, LCH=rp01756en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0010-7824(84)80028-1-
dc.identifier.pmid6548951-
dc.identifier.scopuseid_2-s2.0-0021744186en_US
dc.identifier.volume30en_US
dc.identifier.issue4en_US
dc.identifier.spage363en_US
dc.identifier.epage369en_US
dc.identifier.isiWOS:A1984TT57200007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, SYW=7404255960en_US
dc.identifier.scopusauthoridTang, LCH=7402081111en_US

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