File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Lack of effect of exogenous relaxin on the fertilizing capacity of human spermatozoa

TitleLack of effect of exogenous relaxin on the fertilizing capacity of human spermatozoa
Authors
Issue Date1984
Citation
Ircs Medical Science, 1984, v. 12 n. 10, p. 879-880 How to Cite?
AbstractThe presence of immunoreactive relaxin in human seminal plasma has recently been documented. The potential function for seminal plasma relaxin in spermatozoal physiology was investigated by studying the effect of exogenous relaxin on the in vitro penetration of zona-free hamster ova by human spermatozoa. Biggers, Whitten and Whittingham (BWW) medium-washed spermatozoa from 12 fertile men were incubated with different concentrations (0, 10, 50, 100 and 200 ng/ml) of purified porcine relaxin (NIH-RXN-P1) in BWW at 37° for 5 hours. After incubation, the spermatozoa suspensions were centrifuged, the supernatant discarded, and the pellets resuspended in BWW before insemination of the denuded hamster ova. The penetration rates were scored 6 hours later. Results between the control and the relaxin-treated groups were analyzed statistically. The penetration rate for the non-treated spermatozoa from fertile men was 21%, which was not significantly different (p > 0.05) from the penetration rates observed with the relaxin-treated spermatozoa (10 ng/ml, 24%; 50 ng/ml 27%; 100 ng/ml, 20%; 200 ng/ml, 25%). These findings indicate that exogenous relaxin does not affect the human spermatozoal fertilizing capacity in vitro. The role of seminal plasma relaxin in the human spermatozoal capacitation and fertilization process merits further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/184153
ISSN

 

DC FieldValueLanguage
dc.contributor.authorChan, SYWen_US
dc.contributor.authorTang, LCHen_US
dc.date.accessioned2013-06-25T03:00:47Z-
dc.date.available2013-06-25T03:00:47Z-
dc.date.issued1984en_US
dc.identifier.citationIrcs Medical Science, 1984, v. 12 n. 10, p. 879-880en_US
dc.identifier.issn0305-6651en_US
dc.identifier.urihttp://hdl.handle.net/10722/184153-
dc.description.abstractThe presence of immunoreactive relaxin in human seminal plasma has recently been documented. The potential function for seminal plasma relaxin in spermatozoal physiology was investigated by studying the effect of exogenous relaxin on the in vitro penetration of zona-free hamster ova by human spermatozoa. Biggers, Whitten and Whittingham (BWW) medium-washed spermatozoa from 12 fertile men were incubated with different concentrations (0, 10, 50, 100 and 200 ng/ml) of purified porcine relaxin (NIH-RXN-P1) in BWW at 37° for 5 hours. After incubation, the spermatozoa suspensions were centrifuged, the supernatant discarded, and the pellets resuspended in BWW before insemination of the denuded hamster ova. The penetration rates were scored 6 hours later. Results between the control and the relaxin-treated groups were analyzed statistically. The penetration rate for the non-treated spermatozoa from fertile men was 21%, which was not significantly different (p > 0.05) from the penetration rates observed with the relaxin-treated spermatozoa (10 ng/ml, 24%; 50 ng/ml 27%; 100 ng/ml, 20%; 200 ng/ml, 25%). These findings indicate that exogenous relaxin does not affect the human spermatozoal fertilizing capacity in vitro. The role of seminal plasma relaxin in the human spermatozoal capacitation and fertilization process merits further investigation.en_US
dc.languageengen_US
dc.relation.ispartofIRCS Medical Scienceen_US
dc.titleLack of effect of exogenous relaxin on the fertilizing capacity of human spermatozoaen_US
dc.typeArticleen_US
dc.identifier.emailTang, LCH: lchtang@hku.hken_US
dc.identifier.authorityTang, LCH=rp01756en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0021178928en_US
dc.identifier.volume12en_US
dc.identifier.issue10en_US
dc.identifier.spage879en_US
dc.identifier.epage880en_US
dc.identifier.scopusauthoridChan, SYW=7404255960en_US
dc.identifier.scopusauthoridTang, LCH=7402081111en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats