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Article: Identification of CD44+ cancer stem cells in human gastric cancer

TitleIdentification of CD44+ cancer stem cells in human gastric cancer
Authors
KeywordsCD44
Gastric cancer
Cancer stem cell
Issue Date2013
PublisherHepato-Gastroenterology. The Journal's web site is located at http://www.thieme.de/hepato/index.html
Citation
Hepato-Gastroenterology, 2013, v. 60 n. 124, p. 949-954 How to Cite?
AbstractBackground/Aims: The cancer stem cell hypothesis suggests that tumors are initiated and maintained by cancer stem cells. Methodology: In this study, we identified a subpopulation of CD44+ cells within the tumor of gastric cancer patients, which, upon treatment by chemotherapeutic agent 5-fluorouracil (5-FU), were markedly enriched. Results: In vitro culture of isolated CD44+ subpopulation from gastric tumors by magnetic beads sorting led to formation of gastric spheroid colonies. These colonies retained CD44+ surface marker expression during culture, and were undifferentiated in nature. Subcutaneous injections of CD44+ gastric cancer cells conferred tumorigenicity in SCID mice. Moreover, implantation of CD44+ cells from these established tumors remained tumorigenic in successive passages. Using CD44+ cells isolated from the gastric cell lines AGS and SGC7901, similar results were obtained. Upon enrichment by 5-FU, CD44+ cells harbored increased ALDH expression as compared with CD44- cells. Conclusions: Our results demonstrated for the first time the existence of CD44+ cells within the tumors of gastric cancer patients that are endowed with stem cells properties, and also provide a plausible explanation for chemo-resistance frequently observed in gastric cancer patients. Such findings provide a basis for further studies on targeting this tumorigenic subpopulation for better treatment of gastric cancer patients.
Persistent Identifierhttp://hdl.handle.net/10722/183839
ISSN
2015 Impact Factor: 0.792
2015 SCImago Journal Rankings: 0.446

 

DC FieldValueLanguage
dc.contributor.authorChen, W-
dc.contributor.authorZhang, X-
dc.contributor.authorChu, C-
dc.contributor.authorCheung, WL-
dc.contributor.authorNg, L-
dc.contributor.authorLam, S-
dc.contributor.authorChow, A-
dc.contributor.authorLau, T-
dc.contributor.authorChen, M-
dc.contributor.authorLi, Y-
dc.contributor.authorNie, Y-
dc.contributor.authorWong, BC-
dc.contributor.authorPang, R-
dc.date.accessioned2013-06-18T04:19:34Z-
dc.date.available2013-06-18T04:19:34Z-
dc.date.issued2013-
dc.identifier.citationHepato-Gastroenterology, 2013, v. 60 n. 124, p. 949-954-
dc.identifier.issn0172-6390-
dc.identifier.urihttp://hdl.handle.net/10722/183839-
dc.description.abstractBackground/Aims: The cancer stem cell hypothesis suggests that tumors are initiated and maintained by cancer stem cells. Methodology: In this study, we identified a subpopulation of CD44+ cells within the tumor of gastric cancer patients, which, upon treatment by chemotherapeutic agent 5-fluorouracil (5-FU), were markedly enriched. Results: In vitro culture of isolated CD44+ subpopulation from gastric tumors by magnetic beads sorting led to formation of gastric spheroid colonies. These colonies retained CD44+ surface marker expression during culture, and were undifferentiated in nature. Subcutaneous injections of CD44+ gastric cancer cells conferred tumorigenicity in SCID mice. Moreover, implantation of CD44+ cells from these established tumors remained tumorigenic in successive passages. Using CD44+ cells isolated from the gastric cell lines AGS and SGC7901, similar results were obtained. Upon enrichment by 5-FU, CD44+ cells harbored increased ALDH expression as compared with CD44- cells. Conclusions: Our results demonstrated for the first time the existence of CD44+ cells within the tumors of gastric cancer patients that are endowed with stem cells properties, and also provide a plausible explanation for chemo-resistance frequently observed in gastric cancer patients. Such findings provide a basis for further studies on targeting this tumorigenic subpopulation for better treatment of gastric cancer patients.-
dc.languageeng-
dc.publisherHepato-Gastroenterology. The Journal's web site is located at http://www.thieme.de/hepato/index.html-
dc.relation.ispartofHepato-Gastroenterology-
dc.subjectCD44-
dc.subjectGastric cancer-
dc.subjectCancer stem cell-
dc.titleIdentification of CD44+ cancer stem cells in human gastric cancer-
dc.typeArticle-
dc.identifier.emailNg, L: luing@hku.hk-
dc.identifier.emailWong, BC: bcywong@hku.hk-
dc.identifier.emailPang, R: robertap@hku.hk-
dc.identifier.authorityWong, BC=rp00429-
dc.identifier.authorityPang, R=rp00274-
dc.identifier.doi10.5754/hge12881-
dc.identifier.pmid23478146-
dc.identifier.scopuseid_2-s2.0-84879584481-
dc.identifier.hkuros214994-
dc.identifier.volume60-
dc.identifier.issue124-
dc.identifier.spage949-
dc.identifier.epage954-
dc.publisher.placeGreece-

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