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Article: Gene Expression Profiling Identified High-mobility Group AT-hook (HMGA2) as Being Frequently Upregulated in Esophageal Squamous Cell Carcinoma

TitleGene Expression Profiling Identified High-mobility Group AT-hook (HMGA2) as Being Frequently Upregulated in Esophageal Squamous Cell Carcinoma
Authors
KeywordsEsophageal cancer
Microarray
HMGA2
PEG10
SHANK2
Issue Date2013
PublisherSciencedomain International. The Journal's web site is located at http://www.sciencedomain.org/journal-home.php?id=12
Citation
British Journal of Medicine and Medical Research, 2013, v. 3 n. 2, p. 407-419 How to Cite?
AbstractBackground: Esophageal cancer is one of the most deadly malignancies worldwide and esophageal squamous cell carcinoma (ESCC) is the most frequent type. Methods: We identified up-regulated genes from gene expression profiles of HKESC-4 cell line, its parental tumor tissues, non-tumoral esophageal epithelia and lymph nodes with metastatic carcinoma using Human Genome U133 Plus 2.0 microarray. Results: Four genes [High-mobility group AT-hook 2 (HMGA2), paternally expressed 10 (PEG10), SH3 and multiple ankyrin repeat domains 2 (SHANK2) and WNT1 inducible signaling pathway protein 3 (WISP3)] were selected for further validation with real-time quantitative polymerase chain reaction (qPCR) in a panel of ESCC cell lines and clinical specimens. HMGA2 was found to be overexpressed in the panel of ESCC cell lines tested. By using immunohistochemistry, HMGA2 was found to be up-regulated in 70% of ESCC tissues (21 out of 30 cases). Conclusion: This study demonstrates successful use of gene microarray to identify and reveal HMGA2 as a novel and consistently overexpressed gene in ESCC cell lines and clinical samples.
Persistent Identifierhttp://hdl.handle.net/10722/183837
ISSN

 

DC FieldValueLanguage
dc.contributor.authorCheung, LCMen_US
dc.contributor.authorLai, KKYen_US
dc.contributor.authorLam, AKYen_US
dc.contributor.authorTang, JCOen_US
dc.contributor.authorLuk, JMen_US
dc.contributor.authorLee, NPen_US
dc.contributor.authorChung, Yen_US
dc.contributor.authorTong, DKHen_US
dc.contributor.authorLaw, Sen_US
dc.date.accessioned2013-06-18T04:19:27Z-
dc.date.available2013-06-18T04:19:27Z-
dc.date.issued2013en_US
dc.identifier.citationBritish Journal of Medicine and Medical Research, 2013, v. 3 n. 2, p. 407-419en_US
dc.identifier.issn2231-0614-
dc.identifier.urihttp://hdl.handle.net/10722/183837-
dc.description.abstractBackground: Esophageal cancer is one of the most deadly malignancies worldwide and esophageal squamous cell carcinoma (ESCC) is the most frequent type. Methods: We identified up-regulated genes from gene expression profiles of HKESC-4 cell line, its parental tumor tissues, non-tumoral esophageal epithelia and lymph nodes with metastatic carcinoma using Human Genome U133 Plus 2.0 microarray. Results: Four genes [High-mobility group AT-hook 2 (HMGA2), paternally expressed 10 (PEG10), SH3 and multiple ankyrin repeat domains 2 (SHANK2) and WNT1 inducible signaling pathway protein 3 (WISP3)] were selected for further validation with real-time quantitative polymerase chain reaction (qPCR) in a panel of ESCC cell lines and clinical specimens. HMGA2 was found to be overexpressed in the panel of ESCC cell lines tested. By using immunohistochemistry, HMGA2 was found to be up-regulated in 70% of ESCC tissues (21 out of 30 cases). Conclusion: This study demonstrates successful use of gene microarray to identify and reveal HMGA2 as a novel and consistently overexpressed gene in ESCC cell lines and clinical samples.-
dc.languageengen_US
dc.publisherSciencedomain International. The Journal's web site is located at http://www.sciencedomain.org/journal-home.php?id=12-
dc.relation.ispartofBritish Journal of Medicine and Medical Researchen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEsophageal cancer-
dc.subjectMicroarray-
dc.subjectHMGA2-
dc.subjectPEG10-
dc.subjectSHANK2-
dc.titleGene Expression Profiling Identified High-mobility Group AT-hook (HMGA2) as Being Frequently Upregulated in Esophageal Squamous Cell Carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailLam, AKY: akylam@hkucc.hku.hken_US
dc.identifier.emailTang, JCO: cotang@hkucc.hku.hken_US
dc.identifier.emailLuk, JM: jmluk@hku.hken_US
dc.identifier.emailLee, NP: nikkilee@hku.hken_US
dc.identifier.emailChung, Y: yybms@hkucc.hku.hken_US
dc.identifier.emailTong, DKH: esodtong@hku.hken_US
dc.identifier.emailLaw, S: slaw@hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.identifier.authorityLee, NPY=rp00263en_US
dc.identifier.authorityLaw, SYK=rp00437en_US
dc.description.naturepublished_or_final_version-
dc.identifier.hkuros214884en_US
dc.identifier.volume3en_US
dc.identifier.issue2en_US
dc.identifier.spage407en_US
dc.identifier.epage419en_US
dc.publisher.placeIndia-

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