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Article: Modulation of ion transporter expression in gill mitochondrion-rich cells of eels acclimated to low-Na + or-Cl - freshwater

TitleModulation of ion transporter expression in gill mitochondrion-rich cells of eels acclimated to low-Na + or-Cl - freshwater
Authors
Issue Date2011
Citation
Journal Of Experimental Zoology Part A: Ecological Genetics And Physiology, 2011, v. 315 A n. 7, p. 385-393 How to Cite?
AbstractIn this study, we aimed to establish an experimental model to study the role of the gill mitochondrion-rich cells (MRCs) of freshwater fish in Na + uptake and to examine the effect of adjusting external Na + and Cl - ions on selected ion transporters in gill MRCs. Japanese eels (Anguilla japonica) acclimated to deionized (DI) water for 2 weeks were transferred directly to (a) ion-supplemented artificial freshwater (AF), (b) Na +-deficient AF, or (c) Cl --deficient AF for 2 days. The effects of the transfer on the expression levels of ion transporters in isolated gill cells were investigated. Our data demonstrated that the 2-day acclimation in ion-supplemented AF, Na +-deficient AF, or Cl --deficient AF led to a significant increase in serum osmolarity attributed mainly to an increase in serum Na + and/or Cl - levels when compared with DI-acclimated eel. Significant inductions of V-type H +-ATPase (V-H +-ATPase) and cotransporter (NBC1) mRNA expression in gill MRCs were detected in AF-acclimated fish. In fish acclimated to Na +-deficient AF, mRNA expression levels of V-H +-ATPase, NBC1, and Na +/H +-exchanger-3 (NHE3) were significantly increased in MRCs. Fish acclimated to Cl --deficient AF showed no observable change in expression levels of ion transporters in gill MRCs. In addition, expression levels of ion transporters in pavement cells were stable throughout the 2-day experiments. These data indicate that the level of Na + in freshwater is important for altering the mRNA expression of ion transporters in gill MRCs, which supports the notion that gill MRCs play important roles in freshwater Na + uptake. © 2011 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/183401
ISSN
2015 Impact Factor: 1.226
2015 SCImago Journal Rankings: 0.590
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, WKen_US
dc.contributor.authorChow, SCen_US
dc.contributor.authorLai, KPen_US
dc.contributor.authorAu, DWTen_US
dc.contributor.authorWong, CKen_US
dc.date.accessioned2013-05-27T07:12:37Z-
dc.date.available2013-05-27T07:12:37Z-
dc.date.issued2011en_US
dc.identifier.citationJournal Of Experimental Zoology Part A: Ecological Genetics And Physiology, 2011, v. 315 A n. 7, p. 385-393en_US
dc.identifier.issn1932-5223en_US
dc.identifier.urihttp://hdl.handle.net/10722/183401-
dc.description.abstractIn this study, we aimed to establish an experimental model to study the role of the gill mitochondrion-rich cells (MRCs) of freshwater fish in Na + uptake and to examine the effect of adjusting external Na + and Cl - ions on selected ion transporters in gill MRCs. Japanese eels (Anguilla japonica) acclimated to deionized (DI) water for 2 weeks were transferred directly to (a) ion-supplemented artificial freshwater (AF), (b) Na +-deficient AF, or (c) Cl --deficient AF for 2 days. The effects of the transfer on the expression levels of ion transporters in isolated gill cells were investigated. Our data demonstrated that the 2-day acclimation in ion-supplemented AF, Na +-deficient AF, or Cl --deficient AF led to a significant increase in serum osmolarity attributed mainly to an increase in serum Na + and/or Cl - levels when compared with DI-acclimated eel. Significant inductions of V-type H +-ATPase (V-H +-ATPase) and cotransporter (NBC1) mRNA expression in gill MRCs were detected in AF-acclimated fish. In fish acclimated to Na +-deficient AF, mRNA expression levels of V-H +-ATPase, NBC1, and Na +/H +-exchanger-3 (NHE3) were significantly increased in MRCs. Fish acclimated to Cl --deficient AF showed no observable change in expression levels of ion transporters in gill MRCs. In addition, expression levels of ion transporters in pavement cells were stable throughout the 2-day experiments. These data indicate that the level of Na + in freshwater is important for altering the mRNA expression of ion transporters in gill MRCs, which supports the notion that gill MRCs play important roles in freshwater Na + uptake. © 2011 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Experimental Zoology Part A: Ecological Genetics and Physiologyen_US
dc.subject.meshAcclimatization - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChlorides - Metabolismen_US
dc.subject.meshEels - Metabolismen_US
dc.subject.meshFresh Wateren_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGills - Cytology - Metabolismen_US
dc.subject.meshIonsen_US
dc.subject.meshMitochondria - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshSodium - Deficiency - Metabolismen_US
dc.subject.meshSodium-Bicarbonate Symporters - Genetics - Metabolismen_US
dc.subject.meshSodium-Hydrogen Antiporter - Genetics - Metabolismen_US
dc.subject.meshSymporters - Genetics - Metabolismen_US
dc.subject.meshVacuolar Proton-Translocating Atpases - Genetics - Metabolismen_US
dc.titleModulation of ion transporter expression in gill mitochondrion-rich cells of eels acclimated to low-Na + or-Cl - freshwateren_US
dc.typeArticleen_US
dc.identifier.emailLai, KP: ballllai@hotmail.comen_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jez.681en_US
dc.identifier.pmid21455947-
dc.identifier.scopuseid_2-s2.0-79960057522en_US
dc.identifier.hkuros223769-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960057522&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume315 Aen_US
dc.identifier.issue7en_US
dc.identifier.spage385en_US
dc.identifier.epage393en_US
dc.identifier.isiWOS:000292563700001-
dc.identifier.scopusauthoridTse, WK=11438835100en_US
dc.identifier.scopusauthoridChow, SC=24474072400en_US
dc.identifier.scopusauthoridLai, KP=7402135707en_US
dc.identifier.scopusauthoridAu, DWT=7004909228en_US
dc.identifier.scopusauthoridWong, CK=35276549400en_US

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