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Article: Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou

TitleDioxin-like components in human breast milk collected from Hong Kong and Guangzhou
Authors
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/envres
Citation
Environmental Research, 2004, v. 96 n. 1, p. 88-94 How to Cite?
AbstractThe H4IIE rat hepatoma cell line was employed as a cell model to screen 7-ethoxyresorufin O-deethylase (EROD)-TCDD equivalents (EROD-TEQ) of human breast milk samples collected from Hong Kong and Guangzhou, China. The screening methods employed a 96-well plate spectrofluorometer-EROD assay. For cell-line validation, our results demonstrated a dose-dependent increase in the Ah receptor-mediated response (i.e., CYP1A1 mRNA and EROD) of the cells upon exposure to a number of known Ah receptor agonists, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzothiophene, benzo[a]pyrene, and β-naphthaflavone. TCDD induced CYP1A1 mRNA and EROD was in a close positive correlation (r=0.98). For the screening of dioxin-like compounds, breast milk samples collected during lactation weeks 3-5 were used. One hundred (from Hong Kong) and 48 (from Guangzhou) breast milk samples were assayed, of which 65% and 68% of the samples, respectively, showed detectable dioxin-like activities using the H4IIE cell EROD screening method. For sixty-five samples from Hong Kong the mean EROD-TEQ values ranged from 58.1 to 96.5pg/g of milk fat for those aged 21-36 years while 32 samples from Guangzhou had mean values of 98.8-202.1pg/g of milk fat. In comparisons of the EROD-TEQ values for different age groups from both cities, there were no significant differences (P<0.05). However, the mean and median EROD-TEQ values of the Guangzhou population were in general higher than those of the Hong Kong population. The results of the present study indicate that it is feasible to use the H4IIE cell-line as a model for screening dioxin-like compounds in human breast milk. In addition, the method is rapid and cost-effective, particularly for a routine and high-throughput sample screening analysis, compared to the costly and time-intensive chemical analytical techniques. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/183391
ISSN
2015 Impact Factor: 3.088
2015 SCImago Journal Rankings: 1.452
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KPen_US
dc.contributor.authorLi, Wen_US
dc.contributor.authorXu, Yen_US
dc.contributor.authorWong, MHen_US
dc.contributor.authorWong, CKCen_US
dc.date.accessioned2013-05-27T07:12:30Z-
dc.date.available2013-05-27T07:12:30Z-
dc.date.issued2004en_US
dc.identifier.citationEnvironmental Research, 2004, v. 96 n. 1, p. 88-94en_US
dc.identifier.issn0013-9351en_US
dc.identifier.urihttp://hdl.handle.net/10722/183391-
dc.description.abstractThe H4IIE rat hepatoma cell line was employed as a cell model to screen 7-ethoxyresorufin O-deethylase (EROD)-TCDD equivalents (EROD-TEQ) of human breast milk samples collected from Hong Kong and Guangzhou, China. The screening methods employed a 96-well plate spectrofluorometer-EROD assay. For cell-line validation, our results demonstrated a dose-dependent increase in the Ah receptor-mediated response (i.e., CYP1A1 mRNA and EROD) of the cells upon exposure to a number of known Ah receptor agonists, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzothiophene, benzo[a]pyrene, and β-naphthaflavone. TCDD induced CYP1A1 mRNA and EROD was in a close positive correlation (r=0.98). For the screening of dioxin-like compounds, breast milk samples collected during lactation weeks 3-5 were used. One hundred (from Hong Kong) and 48 (from Guangzhou) breast milk samples were assayed, of which 65% and 68% of the samples, respectively, showed detectable dioxin-like activities using the H4IIE cell EROD screening method. For sixty-five samples from Hong Kong the mean EROD-TEQ values ranged from 58.1 to 96.5pg/g of milk fat for those aged 21-36 years while 32 samples from Guangzhou had mean values of 98.8-202.1pg/g of milk fat. In comparisons of the EROD-TEQ values for different age groups from both cities, there were no significant differences (P<0.05). However, the mean and median EROD-TEQ values of the Guangzhou population were in general higher than those of the Hong Kong population. The results of the present study indicate that it is feasible to use the H4IIE cell-line as a model for screening dioxin-like compounds in human breast milk. In addition, the method is rapid and cost-effective, particularly for a routine and high-throughput sample screening analysis, compared to the costly and time-intensive chemical analytical techniques. © 2003 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/envresen_US
dc.relation.ispartofEnvironmental Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChinaen_US
dc.subject.meshCytochrome P-450 Cyp1a1 - Geneticsen_US
dc.subject.meshDioxins - Analysisen_US
dc.subject.meshEnvironmental Pollutants - Analysisen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMass Screening - Methodsen_US
dc.subject.meshMilk, Human - Metabolismen_US
dc.subject.meshPolychlorinated Biphenyls - Analysisen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.titleDioxin-like components in human breast milk collected from Hong Kong and Guangzhouen_US
dc.typeArticleen_US
dc.identifier.emailLai, KP: ballllai@hotmail.comen_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0013-9351(03)00123-3en_US
dc.identifier.pmid15261788-
dc.identifier.scopuseid_2-s2.0-1542645556en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542645556&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume96en_US
dc.identifier.issue1en_US
dc.identifier.spage88en_US
dc.identifier.epage94en_US
dc.identifier.isiWOS:000222983900011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLai, KP=7402135707en_US
dc.identifier.scopusauthoridLi, W=36068145000en_US
dc.identifier.scopusauthoridXu, Y=7406448808en_US
dc.identifier.scopusauthoridWong, MH=7403908633en_US
dc.identifier.scopusauthoridWong, CKC=35276549400en_US

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