File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL

Conference Paper: Commensals and Periodontopathogens Modulate Immuno-inflammatory Trajectories in HOKs and THP-1

TitleCommensals and Periodontopathogens Modulate Immuno-inflammatory Trajectories in HOKs and THP-1
Authors
KeywordsPeriodontics
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2285 How to Cite?
AbstractObjectives: Periodontal disease is initiated from pathogenic bacteria-mediated uncoordinated inflammatory host response. However, whether commensals and pathogens follow different trajectories during their interactions with host cells is largely unknown. The present study aimed to examine the crosstalk of commensals and periodontopathogens in human oral keratinocytes (HOKs) and THP-1 monocytes. Methods: The commensal periodontal bacteria including Streptococcus mutans (Sm), Streptococcus mitis (Smi) and Streptococcus sanguinis (Ss), and periodontopathogens including Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) were Selected. The interaction of bacteria with HOKs and THP-1 cells was analyzed in a co-culture model using a gradient of multiplicity of infection. The expression of IL-1β, IL-6, IL-8 and TNF-α was assessed using ELISA and real-time qPCR. Cell surface receptors and signal transduction pathways were examined by western blots, and antibody-mediated blocking assays were undertaken for analysis of TLR2 and TLR4. Results: The commensals upregulated the pro-inflammatory cytokines expression in both HOKs and THP-1 monocytes. In contrast, periodontopathogens induced higher levels of cytokines expression in HOKs, while in monocytes Pg down-regulated the TLR-mediated immuno-inflammatory response. THP-1 was more responsive to Smi and Ss in terms of IL-8 and TNF-α production, and to Sm and Smi in terms of IL-1β than the controls and that treated with Pg (p<0.05). In HOKs, both Sm and Pg upregulated IL-6 and IL-8 expression as compared with the control (p<0.05). The cytokines expressions was through TLRs-mediated downstream pathways. Conclusions: This pioneering study suggests that HOKs enable to detect the trajectories of oral commensal and pathogenic bacteria. Whereas, periodontopathogens like Porphyromonas gingivalis may evade innate host defense by paralysing the pro-inflammatory cytokine network of immune cells such as monocytes, thereby achieving proliferation in gingival tissues and contributing to periodontal disease. (Supported by the grants from Hong Kong Research Grants Council to LJJ and HKU fund to CJS).
DescriptionPoster Presenation
Session 276: IADR/Unilever Hatton Awards - Senior Category: Basic Science
Persistent Identifierhttp://hdl.handle.net/10722/183213
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_US
dc.contributor.authorSeneviratne, CJen_US
dc.contributor.authorLau, ASYen_US
dc.contributor.authorWang, CYen_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2013-05-15T01:48:17Z-
dc.date.available2013-05-15T01:48:17Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2285en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/183213-
dc.descriptionPoster Presenation-
dc.descriptionSession 276: IADR/Unilever Hatton Awards - Senior Category: Basic Science-
dc.description.abstractObjectives: Periodontal disease is initiated from pathogenic bacteria-mediated uncoordinated inflammatory host response. However, whether commensals and pathogens follow different trajectories during their interactions with host cells is largely unknown. The present study aimed to examine the crosstalk of commensals and periodontopathogens in human oral keratinocytes (HOKs) and THP-1 monocytes. Methods: The commensal periodontal bacteria including Streptococcus mutans (Sm), Streptococcus mitis (Smi) and Streptococcus sanguinis (Ss), and periodontopathogens including Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) were Selected. The interaction of bacteria with HOKs and THP-1 cells was analyzed in a co-culture model using a gradient of multiplicity of infection. The expression of IL-1β, IL-6, IL-8 and TNF-α was assessed using ELISA and real-time qPCR. Cell surface receptors and signal transduction pathways were examined by western blots, and antibody-mediated blocking assays were undertaken for analysis of TLR2 and TLR4. Results: The commensals upregulated the pro-inflammatory cytokines expression in both HOKs and THP-1 monocytes. In contrast, periodontopathogens induced higher levels of cytokines expression in HOKs, while in monocytes Pg down-regulated the TLR-mediated immuno-inflammatory response. THP-1 was more responsive to Smi and Ss in terms of IL-8 and TNF-α production, and to Sm and Smi in terms of IL-1β than the controls and that treated with Pg (p<0.05). In HOKs, both Sm and Pg upregulated IL-6 and IL-8 expression as compared with the control (p<0.05). The cytokines expressions was through TLRs-mediated downstream pathways. Conclusions: This pioneering study suggests that HOKs enable to detect the trajectories of oral commensal and pathogenic bacteria. Whereas, periodontopathogens like Porphyromonas gingivalis may evade innate host defense by paralysing the pro-inflammatory cytokine network of immune cells such as monocytes, thereby achieving proliferation in gingival tissues and contributing to periodontal disease. (Supported by the grants from Hong Kong Research Grants Council to LJJ and HKU fund to CJS).-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectPeriodontics-
dc.titleCommensals and Periodontopathogens Modulate Immuno-inflammatory Trajectories in HOKs and THP-1en_US
dc.typeConference_Paperen_US
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_US
dc.identifier.emailLau, ASY: asylau@hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.authoritySeneviratne, CJ=rp01372en_US
dc.identifier.authorityLau, ASY=rp00474en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.hkuros214392en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue A: abstract no. 2285en_US
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats