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Article: Uncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysis

TitleUncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysis
Authors
Issue Date2009
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm
Citation
Molecular Genetics And Genomics, 2009, v. 282 n. 6, p. 571-586 How to Cite?
AbstractGlycerol is catabolized by a wide range of microorganisms including Aspergillus species. To identify the transcriptional regulation of glycerol metabolism in Aspergillus, we analyzed data from triplicate batch fermentations of three different Aspergilli (Aspergillus nidulans, Aspergillus oryzae and Aspergillus niger) with glucose and glycerol as carbon sources. Protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having a conserved response across the three Aspergilli. A promoter analysis of the up-regulated genes led to the identification of a conserved binding site for a putative regulator to be 5′-TGCGGGGA-3′, a binding site that is similar to the binding site for Adr1 in yeast and humans. We show that this Adr1 consensus binding sequence was over-represented on promoter regions of several genes in A. nidulans, A. oryzae and A. niger. Our transcriptome analysis indicated that genes involved in ethanol, glycerol, fatty acid, amino acids and formate utilization are putatively regulated by Adr1 in Aspergilli as in Saccharomyces cerevisiae and this transcription factor therefore is likely to be cross-species conserved among Saccharomyces and distant Ascomycetes. Transcriptome data were further used to evaluate the high osmolarity glycerol pathway. All the components of this pathway present in yeast have orthologues in the three Aspergilli studied and its gene expression response suggested that this pathway functions as in S. cerevisiae. Our study clearly demonstrates that cross-species evolutionary comparisons among filamentous fungi, using comparative genomics and transcriptomics, are a powerful tool for uncovering regulatory systems. © 2009 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/181256
ISSN
2015 Impact Factor: 2.622
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSalazar, Men_US
dc.contributor.authorVongsangnak, Wen_US
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorAndersen, MRen_US
dc.contributor.authorNielsen, Jen_US
dc.date.accessioned2013-02-21T02:03:32Z-
dc.date.available2013-02-21T02:03:32Z-
dc.date.issued2009en_US
dc.identifier.citationMolecular Genetics And Genomics, 2009, v. 282 n. 6, p. 571-586en_US
dc.identifier.issn1617-4615en_US
dc.identifier.urihttp://hdl.handle.net/10722/181256-
dc.description.abstractGlycerol is catabolized by a wide range of microorganisms including Aspergillus species. To identify the transcriptional regulation of glycerol metabolism in Aspergillus, we analyzed data from triplicate batch fermentations of three different Aspergilli (Aspergillus nidulans, Aspergillus oryzae and Aspergillus niger) with glucose and glycerol as carbon sources. Protein comparisons and cross-analysis with gene expression data of all three species resulted in the identification of 88 genes having a conserved response across the three Aspergilli. A promoter analysis of the up-regulated genes led to the identification of a conserved binding site for a putative regulator to be 5′-TGCGGGGA-3′, a binding site that is similar to the binding site for Adr1 in yeast and humans. We show that this Adr1 consensus binding sequence was over-represented on promoter regions of several genes in A. nidulans, A. oryzae and A. niger. Our transcriptome analysis indicated that genes involved in ethanol, glycerol, fatty acid, amino acids and formate utilization are putatively regulated by Adr1 in Aspergilli as in Saccharomyces cerevisiae and this transcription factor therefore is likely to be cross-species conserved among Saccharomyces and distant Ascomycetes. Transcriptome data were further used to evaluate the high osmolarity glycerol pathway. All the components of this pathway present in yeast have orthologues in the three Aspergilli studied and its gene expression response suggested that this pathway functions as in S. cerevisiae. Our study clearly demonstrates that cross-species evolutionary comparisons among filamentous fungi, using comparative genomics and transcriptomics, are a powerful tool for uncovering regulatory systems. © 2009 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htmen_US
dc.relation.ispartofMolecular Genetics and Genomicsen_US
dc.subject.meshAspergillus Nidulans - Chemistry - Genetics - Metabolismen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshConserved Sequenceen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Fungalen_US
dc.subject.meshGenome-Wide Association Studyen_US
dc.subject.meshGlycerol - Metabolismen_US
dc.subject.meshOsmotic Pressureen_US
dc.subject.meshStress, Physiologicalen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleUncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysisen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00438-009-0486-yen_US
dc.identifier.pmid19784673-
dc.identifier.scopuseid_2-s2.0-71349083307en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71349083307&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume282en_US
dc.identifier.issue6en_US
dc.identifier.spage571en_US
dc.identifier.epage586en_US
dc.identifier.isiWOS:000271791800002-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridSalazar, M=24067239400en_US
dc.identifier.scopusauthoridVongsangnak, W=24068171600en_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridAndersen, MR=15841796100en_US
dc.identifier.scopusauthoridNielsen, J=7404066338en_US

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