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Article: Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans

TitleSystems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans
Authors
Issue Date2008
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2008, v. 3 n. 12 How to Cite?
AbstractBackground: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. Methodology/Principal Findings: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by overexpression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. Conclusions/Significance: Metabolic flux analysis using 13C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. © 2008 Panagiotou et al.
Persistent Identifierhttp://hdl.handle.net/10722/181253
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorAnderson, MRen_US
dc.contributor.authorGrotkjær, Ten_US
dc.contributor.authorRegueira, TBen_US
dc.contributor.authorHofmann, Gen_US
dc.contributor.authorNielsen, Jen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:31Z-
dc.date.available2013-02-21T02:03:31Z-
dc.date.issued2008en_US
dc.identifier.citationPlos One, 2008, v. 3 n. 12en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/181253-
dc.description.abstractBackground: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. Methodology/Principal Findings: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by overexpression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. Conclusions/Significance: Metabolic flux analysis using 13C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. © 2008 Panagiotou et al.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleSystems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulansen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1371/journal.pone.0003847en_US
dc.identifier.pmid19052639-
dc.identifier.scopuseid_2-s2.0-57349128941en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57349128941&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume3en_US
dc.identifier.issue12en_US
dc.identifier.isiWOS:000265452200004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridAnderson, MR=18133298200en_US
dc.identifier.scopusauthoridGrotkjær, T=6506918583en_US
dc.identifier.scopusauthoridRegueira, TB=25825375400en_US
dc.identifier.scopusauthoridHofmann, G=7202274378en_US
dc.identifier.scopusauthoridNielsen, J=7404066338en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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