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Article: Monitoring novel metabolic pathways using metabolomics and machine learning: Induction of the phosphoketolase pathway in Aspergillus nidulans cultivations

TitleMonitoring novel metabolic pathways using metabolomics and machine learning: Induction of the phosphoketolase pathway in Aspergillus nidulans cultivations
Authors
KeywordsAspergillus Nidulans
Metabolite Profile
Phosphoketolase
Self Organizing Maps (Som)
Issue Date2007
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1573-3882
Citation
Metabolomics, 2007, v. 3 n. 4, p. 503-516 How to Cite?
AbstractThe physiological phenotype of Aspergillus nidulans was determined under different environmental conditions through the quantification of the intracellular and extracellular metabolite pools and clear evidence of the presence of a novel fungal metabolic pathway, the phosphoketolase pathway, was obtained. Induction of the phosphoketolase pathway resulted after blocking the EMP pathway through the deactivation of glyceraldehyde-3-P dehydrogenase (G3PD). Deactivation of G3PD in cultivations of A. nidulans on glucose and xylose led to a 10-fold decrease in the specific growth rate; however, growth could still be sustained solely through the phosphoketolase pathway. Metabolomics and machine learning tools were successfully used to monitor the alteration caused by the inhibition of G3PD in the metabolism of A. nidulans grown on glucose, xylose, acetate as well as mixtures of glucose or xylose with acetate. This is the first study that demonstrates in vivo that the fungal central carbon metabolism includes an active phosphoketolase pathway. © Springer Science+Business Media, LLC 2007.
Persistent Identifierhttp://hdl.handle.net/10722/181247
ISSN
2015 Impact Factor: 3.661
2015 SCImago Journal Rankings: 1.287
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorKouskoumvekaki, Ien_US
dc.contributor.authorJónsdóttir, SOen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:28Z-
dc.date.available2013-02-21T02:03:28Z-
dc.date.issued2007en_US
dc.identifier.citationMetabolomics, 2007, v. 3 n. 4, p. 503-516en_US
dc.identifier.issn1573-3882en_US
dc.identifier.urihttp://hdl.handle.net/10722/181247-
dc.description.abstractThe physiological phenotype of Aspergillus nidulans was determined under different environmental conditions through the quantification of the intracellular and extracellular metabolite pools and clear evidence of the presence of a novel fungal metabolic pathway, the phosphoketolase pathway, was obtained. Induction of the phosphoketolase pathway resulted after blocking the EMP pathway through the deactivation of glyceraldehyde-3-P dehydrogenase (G3PD). Deactivation of G3PD in cultivations of A. nidulans on glucose and xylose led to a 10-fold decrease in the specific growth rate; however, growth could still be sustained solely through the phosphoketolase pathway. Metabolomics and machine learning tools were successfully used to monitor the alteration caused by the inhibition of G3PD in the metabolism of A. nidulans grown on glucose, xylose, acetate as well as mixtures of glucose or xylose with acetate. This is the first study that demonstrates in vivo that the fungal central carbon metabolism includes an active phosphoketolase pathway. © Springer Science+Business Media, LLC 2007.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1573-3882en_US
dc.relation.ispartofMetabolomicsen_US
dc.subjectAspergillus Nidulansen_US
dc.subjectMetabolite Profileen_US
dc.subjectPhosphoketolaseen_US
dc.subjectSelf Organizing Maps (Som)en_US
dc.titleMonitoring novel metabolic pathways using metabolomics and machine learning: Induction of the phosphoketolase pathway in Aspergillus nidulans cultivationsen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11306-007-0061-7en_US
dc.identifier.scopuseid_2-s2.0-36049016078en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36049016078&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume3en_US
dc.identifier.issue4en_US
dc.identifier.spage503en_US
dc.identifier.epage516en_US
dc.identifier.isiWOS:000250918300008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridKouskoumvekaki, I=6602787035en_US
dc.identifier.scopusauthoridJónsdóttir, SO=35566503500en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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