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Article: The influence of different cultivation conditions on the metabolome of Fusarium oxysporum

TitleThe influence of different cultivation conditions on the metabolome of Fusarium oxysporum
Authors
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal Of Biotechnology, 2005, v. 118 n. 3, p. 304-315 How to Cite?
AbstractThe two most widespread pentose sugars found in the biosphere are d-xylose and l-arabinose. They are both potential substrates for ethanol production. The purpose of this study was to better understand the redox constraints imposed to Fusarium oxysporum during utilization of pentoses. In order to increase ethanol yield and decrease by-product formation, nitrate was used as nitrogen source. The use of NADH, the cofactor in denitrification process when using nitrate as a nitrogen source, improved the ethanol yield on xylose to 0.89 mol mol -1 compared to the ethanol yield achieved using ammonium as nitrogen source 0.44 mol mol-1. The improved ethanol yield was followed by a 28% decrease in yield of the by-product xylitol. In order to investigate the metabolic pathway of arabinose and the metabolic limitations for the efficient ethanol production from this sugar, the extracellular and intracellular metabolite profiles were determined under aerobic and anaerobic cultivation conditions. The results of this study clearly show difficulties in channelling of glucose-1-P (G1P) to pentose phosphate pathway (PPP) and reduced NADPH regeneration, suggesting that NADPH becomes a limiting factor for arabinose conversion, resulting in excessive acetate production. Variations of the fungus intracellular amino and non-amino acid pool, under different culture conditions, were evaluated using principal component analysis (PCA). PCA projection of the metabolome data collected from F. oxysporum subjected to environmental perturbations succeeded to visualize different physiological states and the conclusions of this study were that the metabolite profile is unique according to: (1) the carbon source and (2) the oxygen supply, and to a lesser extent to the cultivation phase. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181239
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorChristakopoulos, Pen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:25Z-
dc.date.available2013-02-21T02:03:25Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Biotechnology, 2005, v. 118 n. 3, p. 304-315en_US
dc.identifier.issn0168-1656en_US
dc.identifier.urihttp://hdl.handle.net/10722/181239-
dc.description.abstractThe two most widespread pentose sugars found in the biosphere are d-xylose and l-arabinose. They are both potential substrates for ethanol production. The purpose of this study was to better understand the redox constraints imposed to Fusarium oxysporum during utilization of pentoses. In order to increase ethanol yield and decrease by-product formation, nitrate was used as nitrogen source. The use of NADH, the cofactor in denitrification process when using nitrate as a nitrogen source, improved the ethanol yield on xylose to 0.89 mol mol -1 compared to the ethanol yield achieved using ammonium as nitrogen source 0.44 mol mol-1. The improved ethanol yield was followed by a 28% decrease in yield of the by-product xylitol. In order to investigate the metabolic pathway of arabinose and the metabolic limitations for the efficient ethanol production from this sugar, the extracellular and intracellular metabolite profiles were determined under aerobic and anaerobic cultivation conditions. The results of this study clearly show difficulties in channelling of glucose-1-P (G1P) to pentose phosphate pathway (PPP) and reduced NADPH regeneration, suggesting that NADPH becomes a limiting factor for arabinose conversion, resulting in excessive acetate production. Variations of the fungus intracellular amino and non-amino acid pool, under different culture conditions, were evaluated using principal component analysis (PCA). PCA projection of the metabolome data collected from F. oxysporum subjected to environmental perturbations succeeded to visualize different physiological states and the conclusions of this study were that the metabolite profile is unique according to: (1) the carbon source and (2) the oxygen supply, and to a lesser extent to the cultivation phase. © 2005 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.subject.meshAdaptation, Physiological - Physiologyen_US
dc.subject.meshCell Culture Techniques - Methodsen_US
dc.subject.meshFungal Proteins - Metabolismen_US
dc.subject.meshFusarium - Metabolismen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Fungal - Physiologyen_US
dc.subject.meshProteome - Metabolismen_US
dc.titleThe influence of different cultivation conditions on the metabolome of Fusarium oxysporumen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jbiotec.2005.05.004en_US
dc.identifier.pmid15996783-
dc.identifier.scopuseid_2-s2.0-22544481455en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-22544481455&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume118en_US
dc.identifier.issue3en_US
dc.identifier.spage304en_US
dc.identifier.epage315en_US
dc.identifier.isiWOS:000231124200008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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