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Article: Simultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3 - Growth characteristics and metabolite profiling

TitleSimultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3 - Growth characteristics and metabolite profiling
Authors
KeywordsCellulose
Fusarium Oxysporum
Gaba Shunt
Metabolite Profiling
Simultaneous Saccharification And Fermentation
Issue Date2005
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 2005, v. 36 n. 5-6, p. 693-699 How to Cite?
AbstractThe simultaneous saccharification and fermentation (SSF) of cellulose by Fusarium oxysporum was investigated in the present study. It was found that F. oxysporum grow with a maximum specific growth rate of 0.023 h-1 on cellulose at aerobic conditions and that it can produce ethanol with a volumetric productivity of 0.044 g/L/h and a yield of 0.35 g/g cellulose under anaerobic conditions. The cellulase system in F. oxysporum is well balanced as no cellobiose accumulated. The profile of the phosphorylated intermediates from Pentose Phosphate Pathway (PPP), Embden-Meyerhof-Parnas Pathway (EMP) and the key intermediates of the glycolytic pathway as well as extracellular organic and amino acids were determined during the fermentation in order to investigate the potential metabolic bottlenecks of the process. The high levels of intracellular glucose-1,6-DP a metabolic downstream of phoshoglucomutase also indicates limiting activities of this enzyme and difficulty of glucose to be channelled into biosynthetic and glycolytic pathways. The presence of high levels of γ-aminobutyrate (GABA) under anaerobic conditions suggests a functional GABA bypass and possible block in the Krebs cycle. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181238
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorChristakopoulos, Pen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:25Z-
dc.date.available2013-02-21T02:03:25Z-
dc.date.issued2005en_US
dc.identifier.citationEnzyme And Microbial Technology, 2005, v. 36 n. 5-6, p. 693-699en_US
dc.identifier.issn0141-0229en_US
dc.identifier.urihttp://hdl.handle.net/10722/181238-
dc.description.abstractThe simultaneous saccharification and fermentation (SSF) of cellulose by Fusarium oxysporum was investigated in the present study. It was found that F. oxysporum grow with a maximum specific growth rate of 0.023 h-1 on cellulose at aerobic conditions and that it can produce ethanol with a volumetric productivity of 0.044 g/L/h and a yield of 0.35 g/g cellulose under anaerobic conditions. The cellulase system in F. oxysporum is well balanced as no cellobiose accumulated. The profile of the phosphorylated intermediates from Pentose Phosphate Pathway (PPP), Embden-Meyerhof-Parnas Pathway (EMP) and the key intermediates of the glycolytic pathway as well as extracellular organic and amino acids were determined during the fermentation in order to investigate the potential metabolic bottlenecks of the process. The high levels of intracellular glucose-1,6-DP a metabolic downstream of phoshoglucomutase also indicates limiting activities of this enzyme and difficulty of glucose to be channelled into biosynthetic and glycolytic pathways. The presence of high levels of γ-aminobutyrate (GABA) under anaerobic conditions suggests a functional GABA bypass and possible block in the Krebs cycle. © 2005 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_US
dc.relation.ispartofEnzyme and Microbial Technologyen_US
dc.subjectCelluloseen_US
dc.subjectFusarium Oxysporumen_US
dc.subjectGaba Shunten_US
dc.subjectMetabolite Profilingen_US
dc.subjectSimultaneous Saccharification And Fermentationen_US
dc.titleSimultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3 - Growth characteristics and metabolite profilingen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.enzmictec.2004.12.029en_US
dc.identifier.scopuseid_2-s2.0-14544294432en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14544294432&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume36en_US
dc.identifier.issue5-6en_US
dc.identifier.spage693en_US
dc.identifier.epage699en_US
dc.identifier.isiWOS:000227781000011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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