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Article: Production of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation

TitleProduction of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation
Authors
KeywordsCellulases
Corn Stover
Fusarium Oxysporum
Solid State Culture
Xylanases
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/indcrop
Citation
Industrial Crops And Products, 2003, v. 18 n. 1, p. 37-45 How to Cite?
AbstractCorn stover is an abundant, potential fermentation substrate. Production of cellulolytic and xylanolytic enzymes by the mesophilic fungus Fusarium oxysporum under solid state culture (SSC) on corn stover was enhanced by optimization of the type of nitrogen source, initial moisture level, growth temperature and initial pH of the culture medium. Under these conditions, yields as high as 304, 4.1, 0.140, 1840 and 0.041 U/g of carbon source of endoglucanase, cellobiohydrolase, β-glucosidase, xylanase and β-xylosidase, respectively, were obtained. SCC in a laboratory horizontal bioreactor using the optimized medium allowed the large scale production of the multienzymic system in similar yields. Chromogenic (fluorogenic) 4-methylumbelliferyl β-glycosides of cellobiose and xylobiose were used to characterize the major activities of the multienzyme component, after separation by isoelectric focusing (IEF) electrophoresis. The zymograms indicated one major cellulase and four xylanase activities exhibiting pI values 5 and 5, 6, 7.3, 8.3, respectively. © 2003 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181235
ISSN
2015 Impact Factor: 3.449
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorKekos, Den_US
dc.contributor.authorMacris, BJen_US
dc.contributor.authorChristakopoulos, Pen_US
dc.date.accessioned2013-02-21T02:03:24Z-
dc.date.available2013-02-21T02:03:24Z-
dc.date.issued2003en_US
dc.identifier.citationIndustrial Crops And Products, 2003, v. 18 n. 1, p. 37-45en_US
dc.identifier.issn0926-6690en_US
dc.identifier.urihttp://hdl.handle.net/10722/181235-
dc.description.abstractCorn stover is an abundant, potential fermentation substrate. Production of cellulolytic and xylanolytic enzymes by the mesophilic fungus Fusarium oxysporum under solid state culture (SSC) on corn stover was enhanced by optimization of the type of nitrogen source, initial moisture level, growth temperature and initial pH of the culture medium. Under these conditions, yields as high as 304, 4.1, 0.140, 1840 and 0.041 U/g of carbon source of endoglucanase, cellobiohydrolase, β-glucosidase, xylanase and β-xylosidase, respectively, were obtained. SCC in a laboratory horizontal bioreactor using the optimized medium allowed the large scale production of the multienzymic system in similar yields. Chromogenic (fluorogenic) 4-methylumbelliferyl β-glycosides of cellobiose and xylobiose were used to characterize the major activities of the multienzyme component, after separation by isoelectric focusing (IEF) electrophoresis. The zymograms indicated one major cellulase and four xylanase activities exhibiting pI values 5 and 5, 6, 7.3, 8.3, respectively. © 2003 Elsevier Science B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/indcropen_US
dc.relation.ispartofIndustrial Crops and Productsen_US
dc.subjectCellulasesen_US
dc.subjectCorn Stoveren_US
dc.subjectFusarium Oxysporumen_US
dc.subjectSolid State Cultureen_US
dc.subjectXylanasesen_US
dc.titleProduction of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentationen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0926-6690(03)00018-9en_US
dc.identifier.scopuseid_2-s2.0-0037791760en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037791760&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue1en_US
dc.identifier.spage37en_US
dc.identifier.epage45en_US
dc.identifier.isiWOS:000183480200005-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridKekos, D=7003932669en_US
dc.identifier.scopusauthoridMacris, BJ=7006746072en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US

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