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Article: Combination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsies

TitleCombination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsies
Authors
KeywordsAnaplastic Ganglioglioma
Brain/Cns Cancers
Ex Vivo High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopy
Genomics
Tumor Biomarkers
Issue Date2007
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 199-208 How to Cite?
AbstractAdvancements in the diagnosis and prognosis of brain tumor patients, and thus in their survival and quality of life, can be achieved using biomarkers that facilitate improved tumor typing. We introduce and implement a combinatorial metabolic and molecular approach that applies state-of-the-art, high-resolution magic angle spinning (HRMAS) proton ( 1H) MRS and gene transcriptome profiling to intact brain tumor biopsies, to identify unique biomarker profiles of brain tumors. Our results show that samples as small as 2 mg can be successfully processed, the HRMAS 1H MRS procedure does not result in mRNA degradation, and minute mRNA amounts yield high-quality genomic data. The MRS and genomic analyses demonstrate that CNS tumors have altered levels of specific 1H MRS metabolites that directly correspond to altered expression of Kennedy pathway genes; and exhibit rapid phospholipid turnover, which coincides with upregulation of cell proliferation genes. The data also suggest Sonic Hedgehog pathway (SHH) dysregulation may play a role in anaplastic ganglioglioma pathogenesis. That a strong correlation is seen between the HRMAS 1H MRS and genomic data cross-validates and further demonstrates the biological relevance of the MRS results. Our combined metabolic/molecular MRS/genomic approach provides insights into the biology of anaplastic ganglioglioma and a new potential tumor typing methodology that could aid neurologists and neurosurgeons to improve the diagnosis, treatment, and ongoing evaluation of brain tumor patients.
Persistent Identifierhttp://hdl.handle.net/10722/180729
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
References

 

DC FieldValueLanguage
dc.contributor.authorTzika, AAen_US
dc.contributor.authorAstrakas, Len_US
dc.contributor.authorCao, Hen_US
dc.contributor.authorMintzopoulos, Den_US
dc.contributor.authorAndronesi, OCen_US
dc.contributor.authorMindrinos, Men_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorRahme, LGen_US
dc.contributor.authorBlekas, KDen_US
dc.contributor.authorLikas, ACen_US
dc.contributor.authorGalatsanos, NPen_US
dc.contributor.authorCarroll, RSen_US
dc.contributor.authorBlack, PMen_US
dc.date.accessioned2013-01-28T01:42:04Z-
dc.date.available2013-01-28T01:42:04Z-
dc.date.issued2007en_US
dc.identifier.citationInternational Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 199-208en_US
dc.identifier.issn1107-3756en_US
dc.identifier.urihttp://hdl.handle.net/10722/180729-
dc.description.abstractAdvancements in the diagnosis and prognosis of brain tumor patients, and thus in their survival and quality of life, can be achieved using biomarkers that facilitate improved tumor typing. We introduce and implement a combinatorial metabolic and molecular approach that applies state-of-the-art, high-resolution magic angle spinning (HRMAS) proton ( 1H) MRS and gene transcriptome profiling to intact brain tumor biopsies, to identify unique biomarker profiles of brain tumors. Our results show that samples as small as 2 mg can be successfully processed, the HRMAS 1H MRS procedure does not result in mRNA degradation, and minute mRNA amounts yield high-quality genomic data. The MRS and genomic analyses demonstrate that CNS tumors have altered levels of specific 1H MRS metabolites that directly correspond to altered expression of Kennedy pathway genes; and exhibit rapid phospholipid turnover, which coincides with upregulation of cell proliferation genes. The data also suggest Sonic Hedgehog pathway (SHH) dysregulation may play a role in anaplastic ganglioglioma pathogenesis. That a strong correlation is seen between the HRMAS 1H MRS and genomic data cross-validates and further demonstrates the biological relevance of the MRS results. Our combined metabolic/molecular MRS/genomic approach provides insights into the biology of anaplastic ganglioglioma and a new potential tumor typing methodology that could aid neurologists and neurosurgeons to improve the diagnosis, treatment, and ongoing evaluation of brain tumor patients.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_US
dc.relation.ispartofInternational Journal of Molecular Medicineen_US
dc.subjectAnaplastic Gangliogliomaen_US
dc.subjectBrain/Cns Cancersen_US
dc.subjectEx Vivo High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopyen_US
dc.subjectGenomicsen_US
dc.subjectTumor Biomarkersen_US
dc.titleCombination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsiesen_US
dc.typeArticleen_US
dc.identifier.emailZhang, J: jzhang1@hku.hken_US
dc.identifier.authorityZhang, J=rp01713en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-35448970283en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35448970283&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue2en_US
dc.identifier.spage199en_US
dc.identifier.epage208en_US
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridTzika, AA=7003635500en_US
dc.identifier.scopusauthoridAstrakas, L=6603155897en_US
dc.identifier.scopusauthoridCao, H=36829765800en_US
dc.identifier.scopusauthoridMintzopoulos, D=16070342600en_US
dc.identifier.scopusauthoridAndronesi, OC=6506599452en_US
dc.identifier.scopusauthoridMindrinos, M=6603048545en_US
dc.identifier.scopusauthoridZhang, J=22137260600en_US
dc.identifier.scopusauthoridRahme, LG=6603919311en_US
dc.identifier.scopusauthoridBlekas, KD=6603665613en_US
dc.identifier.scopusauthoridLikas, AC=7003844484en_US
dc.identifier.scopusauthoridGalatsanos, NP=35562970900en_US
dc.identifier.scopusauthoridCarroll, RS=7201449106en_US
dc.identifier.scopusauthoridBlack, PM=17633338700en_US

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