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Article: Sequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis

TitleSequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesis
Authors
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 319 n. 1, p. 235-239 How to Cite?
AbstractTreatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPβ is rapidly expressed, followed by C/EBPα and PPARγ. C/EBPα and PPARγ then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPβ activates transcription of the C/EBPα and PPARγ genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPβ is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for ∼14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPβ binds to C/EBP regulatory elements in the proximal promoters of the C/EBPα and PPARγ genes. After an additional delay ChIP analysis showed that C/EBPα binds to its own promoter and to the promoters of the PPARγ and 422/aP2 genes. These findings support the view that once expressed, C/EBPα is responsible for maintaining the expression of PPARγ and C/EBPα, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPβ, C/EBPα, and PPARγ participate in a cascade during adipogenesis. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/180724
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, QQen_US
dc.contributor.authorZhang, JWen_US
dc.contributor.authorLane, MDen_US
dc.date.accessioned2013-01-28T01:41:59Z-
dc.date.available2013-01-28T01:41:59Z-
dc.date.issued2004en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 319 n. 1, p. 235-239en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/180724-
dc.description.abstractTreatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPβ is rapidly expressed, followed by C/EBPα and PPARγ. C/EBPα and PPARγ then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPβ activates transcription of the C/EBPα and PPARγ genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPβ is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for ∼14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPβ binds to C/EBP regulatory elements in the proximal promoters of the C/EBPα and PPARγ genes. After an additional delay ChIP analysis showed that C/EBPα binds to its own promoter and to the promoters of the PPARγ and 422/aP2 genes. These findings support the view that once expressed, C/EBPα is responsible for maintaining the expression of PPARγ and C/EBPα, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPβ, C/EBPα, and PPARγ participate in a cascade during adipogenesis. © 2004 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.mesh3T3-L1 Cellsen_US
dc.subject.meshAdipocytes - Metabolismen_US
dc.subject.meshAdipose Tissue - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCcaat-Enhancer-Binding Protein-Alpha - Geneticsen_US
dc.subject.meshCcaat-Enhancer-Binding Protein-Beta - Geneticsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshChromatin - Chemistry - Metabolismen_US
dc.subject.meshDna - Chemistryen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshMiceen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshReceptors, Cytoplasmic And Nuclear - Geneticsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleSequential gene promoter interactions of C/EBPβ, C/EBPα, and PPARγ during adipogenesisen_US
dc.typeArticleen_US
dc.identifier.emailZhang, JW: jzhang1@hku.hken_US
dc.identifier.authorityZhang, JW=rp01713en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbrc.2004.04.176en_US
dc.identifier.pmid15158467-
dc.identifier.scopuseid_2-s2.0-2442680067en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442680067&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume319en_US
dc.identifier.issue1en_US
dc.identifier.spage235en_US
dc.identifier.epage239en_US
dc.identifier.isiWOS:000221997300034-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTang, QQ=7201631934en_US
dc.identifier.scopusauthoridZhang, JW=7601339417en_US
dc.identifier.scopusauthoridLane, MD=7401977437en_US

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