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Article: Direct priming and cross-priming contribute differentially to the induction of CD8 + CTL following exposure to vaccinia virus via different routes

TitleDirect priming and cross-priming contribute differentially to the induction of CD8 + CTL following exposure to vaccinia virus via different routes
Authors
Issue Date2002
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 2002, v. 169 n. 8, p. 4222-4229 How to Cite?
AbstractTo explore the relative importance of direct presentation vs cross-priming in the induction of CTL responses to viruses and viral vectors, we generated a recombinant vaccinia vector, vUS11, expressing the human CMV (HCMV) protein US11. US11 dislocates most allelic forms of human and murine MHC class I heavy chains from the lumen of the endoplasmic reticulum into the cytosol, where they are degraded by proteasomes. Expression of US11 dramatically decreased the presentation of viral Ag and CTL recognition of infected cells in vitro without significantly reducing total cell surface MHC class I levels. However, because US11 is an endoplasmic reticulum resident membrane protein, it cannot block presentation by non-infected cells that take up Ag through the cross-priming pathway. We show that the expression of US11 strongly inhibits the induction of primary CD8 + CTLs when the infection occurs via the i.p. or i.v. route, demonstrating that direct priming is critical for the induction of CTL responses to viral infections introduced via these routes. This effect is less dramatic following i.m. infection and is minimal after s.c. or intradermal infection. Thus, classic MHC class I Ag presentation and cross-priming contribute differentially to the induction of CD8 + CTLs following exposure to vaccinia virus via different routes.
Persistent Identifierhttp://hdl.handle.net/10722/180720
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, Xen_US
dc.contributor.authorWong, SBJen_US
dc.contributor.authorBuck, CBen_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorSiliciano, RFen_US
dc.date.accessioned2013-01-28T01:41:57Z-
dc.date.available2013-01-28T01:41:57Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Immunology, 2002, v. 169 n. 8, p. 4222-4229en_US
dc.identifier.issn0022-1767en_US
dc.identifier.urihttp://hdl.handle.net/10722/180720-
dc.description.abstractTo explore the relative importance of direct presentation vs cross-priming in the induction of CTL responses to viruses and viral vectors, we generated a recombinant vaccinia vector, vUS11, expressing the human CMV (HCMV) protein US11. US11 dislocates most allelic forms of human and murine MHC class I heavy chains from the lumen of the endoplasmic reticulum into the cytosol, where they are degraded by proteasomes. Expression of US11 dramatically decreased the presentation of viral Ag and CTL recognition of infected cells in vitro without significantly reducing total cell surface MHC class I levels. However, because US11 is an endoplasmic reticulum resident membrane protein, it cannot block presentation by non-infected cells that take up Ag through the cross-priming pathway. We show that the expression of US11 strongly inhibits the induction of primary CD8 + CTLs when the infection occurs via the i.p. or i.v. route, demonstrating that direct priming is critical for the induction of CTL responses to viral infections introduced via these routes. This effect is less dramatic following i.m. infection and is minimal after s.c. or intradermal infection. Thus, classic MHC class I Ag presentation and cross-priming contribute differentially to the induction of CD8 + CTLs following exposure to vaccinia virus via different routes.en_US
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigen Presentation - Geneticsen_US
dc.subject.meshAntigens, Viral - Immunology - Metabolismen_US
dc.subject.meshCytotoxicity, Immunologic - Geneticsen_US
dc.subject.meshEpitopes, T-Lymphocyte - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenetic Vectors - Administration & Dosage - Biosynthesis - Immunologyen_US
dc.subject.meshHistocompatibility Antigens Class I - Immunology - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshInjections, Intradermalen_US
dc.subject.meshInjections, Intramuscularen_US
dc.subject.meshInjections, Intraperitonealen_US
dc.subject.meshInjections, Intravenousen_US
dc.subject.meshInjections, Subcutaneousen_US
dc.subject.meshLymphocyte Activation - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Aen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshRna-Binding Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshT-Lymphocytes, Cytotoxic - Immunology - Virologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshVaccination - Methodsen_US
dc.subject.meshVaccines, Synthetic - Administration & Dosage - Chemistry - Genetics - Immunologyen_US
dc.subject.meshVaccinia Virus - Genetics - Immunologyen_US
dc.subject.meshViral Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshViral Vaccines - Administration & Dosage - Chemical Synthesis - Genetics - Immunologyen_US
dc.titleDirect priming and cross-priming contribute differentially to the induction of CD8 + CTL following exposure to vaccinia virus via different routesen_US
dc.typeArticleen_US
dc.identifier.emailZhang, J: jzhang1@hku.hken_US
dc.identifier.authorityZhang, J=rp01713en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.4049/jimmunol.169.8.4222-
dc.identifier.pmid12370352-
dc.identifier.scopuseid_2-s2.0-0037108514en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037108514&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume169en_US
dc.identifier.issue8en_US
dc.identifier.spage4222en_US
dc.identifier.epage4229en_US
dc.identifier.isiWOS:000178512000022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShen, X=7402721623en_US
dc.identifier.scopusauthoridWong, SBJ=55451148300en_US
dc.identifier.scopusauthoridBuck, CB=7102927846en_US
dc.identifier.scopusauthoridZhang, J=7601339417en_US
dc.identifier.scopusauthoridSiliciano, RF=7005539788en_US

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