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Article: Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions

TitleDetection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions
Authors
Poon, LLMPeiris, JSM
KeywordsBat coronaviruses
Molecular detection
Novel coronaviruses
RT-PCR
Issue Date2008
Citation
Methods In Molecular Biology, 2008, v. 454, p. 13-26 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-59745-181-9-2
AbstractThe zoonotic transmission of SARS coronavirus from animals to humans revealed the potential impact of coronaviruses on mankind. This incident also triggered several surveillance programs to hunt for novel coronaviruses in human and wildlife populations. Using classical RT-PCR assays that target a highly conserved sequence among coronaviruses, we identified the first coronaviruses in bats. These assays and the cloning and sequencing of the PCR products are described in this chapter. Using the same approach in our subsequent studies, we further detected several novel coronaviruses in bats. These findings highlighted the fact that bats are important reservoirs for coronaviruses. © 2008 Humana Press.
Persistent Identifierhttp://hdl.handle.net/10722/179818
ISSN
1064-3745
2020 SCImago Journal Rankings: 0.711
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorPeiris, JSMen_US
dc.date.accessioned2012-12-19T10:05:10Z-
dc.date.available2012-12-19T10:05:10Z-
dc.date.issued2008en_US
dc.identifier.citationMethods In Molecular Biology, 2008, v. 454, p. 13-26en_US
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/179818-
dc.description.abstractThe zoonotic transmission of SARS coronavirus from animals to humans revealed the potential impact of coronaviruses on mankind. This incident also triggered several surveillance programs to hunt for novel coronaviruses in human and wildlife populations. Using classical RT-PCR assays that target a highly conserved sequence among coronaviruses, we identified the first coronaviruses in bats. These assays and the cloning and sequencing of the PCR products are described in this chapter. Using the same approach in our subsequent studies, we further detected several novel coronaviruses in bats. These findings highlighted the fact that bats are important reservoirs for coronaviruses. © 2008 Humana Press.en_US
dc.languageengen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.subjectBat coronaviruses-
dc.subjectMolecular detection-
dc.subjectNovel coronaviruses-
dc.subjectRT-PCR-
dc.subject.meshAnimalsen_US
dc.subject.meshChiroptera - Virologyen_US
dc.subject.meshCoronavirus - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.titleDetection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactionsen_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/978-1-59745-181-9-2en_US
dc.identifier.pmid19057871-
dc.identifier.scopuseid_2-s2.0-84934435659en_US
dc.identifier.hkuros164361-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62449277819&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume454en_US
dc.identifier.spage13en_US
dc.identifier.epage26en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.issnl1064-3745-

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