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Article: Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray

TitleGeneric detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray
Authors
Issue Date2007
Citation
Journal Of Clinical Microbiology, 2007, v. 45 n. 3, p. 1049-1052 How to Cite?
AbstractA nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children sufering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179798
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDe Souza Luna, LKen_US
dc.contributor.authorHeiser, Ven_US
dc.contributor.authorRegamey, Nen_US
dc.contributor.authorPanning, Men_US
dc.contributor.authorDrexler, JFen_US
dc.contributor.authorMulangu, Sen_US
dc.contributor.authorPoon, Len_US
dc.contributor.authorBaumgarte, Sen_US
dc.contributor.authorHaijema, BJen_US
dc.contributor.authorKaiser, Len_US
dc.contributor.authorDrosten, Cen_US
dc.date.accessioned2012-12-19T10:04:52Z-
dc.date.available2012-12-19T10:04:52Z-
dc.date.issued2007en_US
dc.identifier.citationJournal Of Clinical Microbiology, 2007, v. 45 n. 3, p. 1049-1052en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/179798-
dc.description.abstractA nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children sufering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshCoronavirus - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshCoronavirus 229E, Human - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshCoronavirus Oc43, Human - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshOligonucleotide Array Sequence Analysis - Methodsen_US
dc.subject.meshRna, Viral - Analysis - Geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.subject.meshSars Virus - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshSpecies Specificityen_US
dc.titleGeneric detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarrayen_US
dc.typeArticleen_US
dc.identifier.emailPoon, L: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, L=rp00484en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JCM.02426-06en_US
dc.identifier.pmid17229859-
dc.identifier.pmcidPMC1829107-
dc.identifier.scopuseid_2-s2.0-33947214129en_US
dc.identifier.hkuros134208-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947214129&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue3en_US
dc.identifier.spage1049en_US
dc.identifier.epage1052en_US
dc.identifier.isiWOS:000245071500058-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDe Souza Luna, LK=14024020500en_US
dc.identifier.scopusauthoridHeiser, V=6603626178en_US
dc.identifier.scopusauthoridRegamey, N=6602904489en_US
dc.identifier.scopusauthoridPanning, M=6602216657en_US
dc.identifier.scopusauthoridDrexler, JF=15753438200en_US
dc.identifier.scopusauthoridMulangu, S=12752259600en_US
dc.identifier.scopusauthoridPoon, L=7005441747en_US
dc.identifier.scopusauthoridBaumgarte, S=23396171800en_US
dc.identifier.scopusauthoridHaijema, BJ=6602899361en_US
dc.identifier.scopusauthoridKaiser, L=7202547270en_US
dc.identifier.scopusauthoridDrosten, C=7003813990en_US

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