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Article: Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes

TitleInsect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes
Authors
Issue Date2005
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/pestmanagementscience
Citation
Pest Management Science, 2005, v. 61 n. 4, p. 390-396 How to Cite?
AbstractMolecular genetic analysis and insect bioassay of transgenic indica rice 'Zhuxian B' plants carrying snowdrop lectin gene (gna) and soybean trypsin inhibitor gene (sbti) were investigated in detail. PCR, 'dot' blot and PCR-Southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to R3 progeny in most lines tested. Some transgenic lines exhibited Mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) or other aberrant segregation patterns. The segregation patterns of gna gene crossed between R2 and R3 progeny. In half of transgenic R3 lines, gna and sbti transgenes co-segregated. Two independent homozygous lines expressing double transgenes were identified in R3 progeny. Southern blot analysis demonstrated that the copy numbers of integrated gna and sbti transgenes varied from one to ten in different lines. Insect bioassay data showed that most transgenic plants had better resistance to both Nilaparvata lugens (Ståhl) and Cnaphalocrocis medinalis (Guenée) than wild-type plants. The insect resistance of transgenic lines increased with the increase in transgene positive ratio in most of the transgenic lines. In all, we obtained nine lines of R3 transgenic plants, including one pure line, which had better resistance to both N lugens and C medinalis than wild-type plants. © 2004 Society of Chemical Industry.
Persistent Identifierhttp://hdl.handle.net/10722/179781
ISSN
2015 Impact Factor: 2.811
2015 SCImago Journal Rankings: 1.258
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Gen_US
dc.contributor.authorXu, Xen_US
dc.contributor.authorXing, Hen_US
dc.contributor.authorZhu, Hen_US
dc.contributor.authorFan, Qen_US
dc.date.accessioned2012-12-19T10:04:33Z-
dc.date.available2012-12-19T10:04:33Z-
dc.date.issued2005en_US
dc.identifier.citationPest Management Science, 2005, v. 61 n. 4, p. 390-396en_US
dc.identifier.issn1526-498Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179781-
dc.description.abstractMolecular genetic analysis and insect bioassay of transgenic indica rice 'Zhuxian B' plants carrying snowdrop lectin gene (gna) and soybean trypsin inhibitor gene (sbti) were investigated in detail. PCR, 'dot' blot and PCR-Southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to R3 progeny in most lines tested. Some transgenic lines exhibited Mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) or other aberrant segregation patterns. The segregation patterns of gna gene crossed between R2 and R3 progeny. In half of transgenic R3 lines, gna and sbti transgenes co-segregated. Two independent homozygous lines expressing double transgenes were identified in R3 progeny. Southern blot analysis demonstrated that the copy numbers of integrated gna and sbti transgenes varied from one to ten in different lines. Insect bioassay data showed that most transgenic plants had better resistance to both Nilaparvata lugens (Ståhl) and Cnaphalocrocis medinalis (Guenée) than wild-type plants. The insect resistance of transgenic lines increased with the increase in transgene positive ratio in most of the transgenic lines. In all, we obtained nine lines of R3 transgenic plants, including one pure line, which had better resistance to both N lugens and C medinalis than wild-type plants. © 2004 Society of Chemical Industry.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/pestmanagementscienceen_US
dc.relation.ispartofPest Management Scienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshGalanthus - Geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHemipteraen_US
dc.subject.meshInsectsen_US
dc.subject.meshMannose-Binding Lectins - Genetics - Metabolismen_US
dc.subject.meshOryza Sativa - Genetics - Parasitologyen_US
dc.subject.meshPlant Lectins - Genetics - Metabolismen_US
dc.subject.meshPlants, Genetically Modified - Parasitologyen_US
dc.subject.meshSoybeans - Geneticsen_US
dc.subject.meshTransgenesen_US
dc.subject.meshTrypsin Inhibitors - Genetics - Metabolismen_US
dc.titleInsect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenesen_US
dc.typeArticleen_US
dc.identifier.emailZhu, H: zhuhch@hku.hken_US
dc.identifier.authorityZhu, H=rp01535en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ps.990en_US
dc.identifier.pmid15593292-
dc.identifier.scopuseid_2-s2.0-16244394834en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16244394834&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume61en_US
dc.identifier.issue4en_US
dc.identifier.spage390en_US
dc.identifier.epage396en_US
dc.identifier.isiWOS:000228017000008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLi, G=7407051300en_US
dc.identifier.scopusauthoridXu, X=35228207200en_US
dc.identifier.scopusauthoridXing, H=8519673700en_US
dc.identifier.scopusauthoridZhu, H=25724029300en_US
dc.identifier.scopusauthoridFan, Q=36098887700en_US
dc.identifier.citeulike130508-

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