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Article: A longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

TitleA longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka
Authors
Issue Date1993
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYG
Citation
Epidemiology And Infection, 1993, v. 110 n. 2, p. 197-208 How to Cite?
AbstractTwo cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by Vero cytotoxin (VT) producing Escherichia coli (VTEC) occurred. Ninety-one of 171 (53%) faecal specimens were VTEC positive and 20-80% of animals at any given time excreted VTEC. Of 104 VTEC strains studies further, 6 different serogroups (O 22.H16; O 25.H5; O 49.H-; O 86.H26; O 88.H25; O 153.H12) and an untypable strain (O @?.H21) were identified. All strains belonging to the same serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2. LTI or II and a probe (CVD419) derived from a plasmid carried by entero-haemorrhagic Escherichia coli O 157.H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period. Sixty percent of the strains hybridized with the probe for VT1, 4% with the probe for VT2, and 36% with both probes. Faecal VTEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age, but no characterisitic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 was detected in some animals, but faecal VT1 VTEC excretion persisted in spite of seroconversion. VT1 seroconversion was not associated with diarrhoea. A serological response to VT2 was not detected even in those animals excreting VT2 VTEC in the faeces.
Persistent Identifierhttp://hdl.handle.net/10722/179747
ISSN
2015 Impact Factor: 2.515
2015 SCImago Journal Rankings: 1.320
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTokhi, AMen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorScotland, SMen_US
dc.contributor.authorWillshaw, GAen_US
dc.contributor.authorSmith, HRen_US
dc.contributor.authorCheasty, Ten_US
dc.date.accessioned2012-12-19T10:04:16Z-
dc.date.available2012-12-19T10:04:16Z-
dc.date.issued1993en_US
dc.identifier.citationEpidemiology And Infection, 1993, v. 110 n. 2, p. 197-208en_US
dc.identifier.issn0950-2688en_US
dc.identifier.urihttp://hdl.handle.net/10722/179747-
dc.description.abstractTwo cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by Vero cytotoxin (VT) producing Escherichia coli (VTEC) occurred. Ninety-one of 171 (53%) faecal specimens were VTEC positive and 20-80% of animals at any given time excreted VTEC. Of 104 VTEC strains studies further, 6 different serogroups (O 22.H16; O 25.H5; O 49.H-; O 86.H26; O 88.H25; O 153.H12) and an untypable strain (O @?.H21) were identified. All strains belonging to the same serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2. LTI or II and a probe (CVD419) derived from a plasmid carried by entero-haemorrhagic Escherichia coli O 157.H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period. Sixty percent of the strains hybridized with the probe for VT1, 4% with the probe for VT2, and 36% with both probes. Faecal VTEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age, but no characterisitic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 was detected in some animals, but faecal VT1 VTEC excretion persisted in spite of seroconversion. VT1 seroconversion was not associated with diarrhoea. A serological response to VT2 was not detected even in those animals excreting VT2 VTEC in the faeces.en_US
dc.languageengen_US
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYGen_US
dc.relation.ispartofEpidemiology and Infectionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial - Immunologyen_US
dc.subject.meshBacterial Toxins - Biosynthesis - Immunologyen_US
dc.subject.meshCattleen_US
dc.subject.meshCattle Diseases - Epidemiology - Microbiologyen_US
dc.subject.meshCytotoxins - Biosynthesis - Immunologyen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshDna Probesen_US
dc.subject.meshDiarrhea - Epidemiology - Veterinaryen_US
dc.subject.meshEnterotoxins - Biosynthesis - Immunologyen_US
dc.subject.meshEscherichia Coli - Genetics - Immunology - Metabolismen_US
dc.subject.meshEscherichia Coli Infections - Epidemiology - Veterinaryen_US
dc.subject.meshFeces - Microbiologyen_US
dc.subject.meshLongitudinal Studiesen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPrevalenceen_US
dc.subject.meshShiga Toxin 1en_US
dc.subject.meshSri Lanka - Epidemiologyen_US
dc.subject.meshVirulenceen_US
dc.titleA longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lankaen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1017/S0950268800068114-
dc.identifier.pmid8472764-
dc.identifier.scopuseid_2-s2.0-0027323901en_US
dc.identifier.volume110en_US
dc.identifier.issue2en_US
dc.identifier.spage197en_US
dc.identifier.epage208en_US
dc.identifier.isiWOS:A1993KX49900002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTokhi, AM=6506577935en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridScotland, SM=7004747820en_US
dc.identifier.scopusauthoridWillshaw, GA=7005289886en_US
dc.identifier.scopusauthoridSmith, HR=10339051700en_US
dc.identifier.scopusauthoridCheasty, T=7007147387en_US

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