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Article: Target antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolates

TitleTarget antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolates
Authors
Issue Date1990
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 1990, v. 144 n. 11, p. 4376-4383 How to Cite?
AbstractA panel of 20 anti-Plasmodium vivax female gamete mAb has been established and was characterized with respect to their transmission-blocking properties in membrane-feeding experiments and their target Ag identified. Seven mAb suppressed the infectivity of P. vivax parasites to Anopheles tesselatus mosquitoes. The m.w. of the Ag recognized by these mAb were ascertained by SDS-PAGE and Western blots. Three sets of polypeptides of low M(r) - 20, 24, and a doublet of 37/42 kDa - have been defined as traget Ag of transmission-blocking antibodies of P. vivax. All epitopes of these target Ag were found to be dependent on the tertiary conformational structure of the Ag. Polymorphism of target Ag of transmission-blocking immunity was investigated in over 30 natural isolates of P. vivax in Sri Lanka based on the reactivity of a mAb with an isolate as assessed by the indirect immunofluorescent test with the use of live extracellular female gametes, and in Western blots with the use of extracted gametes. The functional consequences of antigenic polymorphism on immunity was investigated in transmission-blocking assays by using membrane-feeding experiments. A majority of target Ag of transmission-blocking immunity were found to be polymorphic, exhibiting size as well as epitope polymorphism. Results indicate that failure of a mAb to affect the inefectivity of a parasite isolate of P. vivax to mosquitoes can be caused by polymorphism of the target Ag among isolates.
Persistent Identifierhttp://hdl.handle.net/10722/179740
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPremawansa, Sen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorPerera Lakshman, KLRen_US
dc.contributor.authorAriyaratne, Gen_US
dc.contributor.authorCarter, Ren_US
dc.contributor.authorMendis, KNen_US
dc.date.accessioned2012-12-19T10:04:13Z-
dc.date.available2012-12-19T10:04:13Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Immunology, 1990, v. 144 n. 11, p. 4376-4383en_US
dc.identifier.issn0022-1767en_US
dc.identifier.urihttp://hdl.handle.net/10722/179740-
dc.description.abstractA panel of 20 anti-Plasmodium vivax female gamete mAb has been established and was characterized with respect to their transmission-blocking properties in membrane-feeding experiments and their target Ag identified. Seven mAb suppressed the infectivity of P. vivax parasites to Anopheles tesselatus mosquitoes. The m.w. of the Ag recognized by these mAb were ascertained by SDS-PAGE and Western blots. Three sets of polypeptides of low M(r) - 20, 24, and a doublet of 37/42 kDa - have been defined as traget Ag of transmission-blocking antibodies of P. vivax. All epitopes of these target Ag were found to be dependent on the tertiary conformational structure of the Ag. Polymorphism of target Ag of transmission-blocking immunity was investigated in over 30 natural isolates of P. vivax in Sri Lanka based on the reactivity of a mAb with an isolate as assessed by the indirect immunofluorescent test with the use of live extracellular female gametes, and in Western blots with the use of extracted gametes. The functional consequences of antigenic polymorphism on immunity was investigated in transmission-blocking assays by using membrane-feeding experiments. A majority of target Ag of transmission-blocking immunity were found to be polymorphic, exhibiting size as well as epitope polymorphism. Results indicate that failure of a mAb to affect the inefectivity of a parasite isolate of P. vivax to mosquitoes can be caused by polymorphism of the target Ag among isolates.en_US
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshAntibodies, Protozoan - Genetics - Immunologyen_US
dc.subject.meshAntigens, Protozoan - Genetics - Immunologyen_US
dc.subject.meshAntigens, Surface - Immunologyen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCulicidae - Parasitologyen_US
dc.subject.meshEpitopesen_US
dc.subject.meshFemaleen_US
dc.subject.meshMalaria - Immunologyen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshOvum - Immunologyen_US
dc.subject.meshPlasmodium Vivax - Immunology - Pathogenicityen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.titleTarget antigens of transmission blocking immunity of Plasmodium vivax malaria. Characterization and polymorphism in natural parasite isolatesen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1692862-
dc.identifier.scopuseid_2-s2.0-0025284774en_US
dc.identifier.volume144en_US
dc.identifier.issue11en_US
dc.identifier.spage4376en_US
dc.identifier.epage4383en_US
dc.identifier.isiWOS:A1990DF84400043-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPremawansa, S=6601964639en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridPerera Lakshman, KLR=6504713351en_US
dc.identifier.scopusauthoridAriyaratne, G=7801423153en_US
dc.identifier.scopusauthoridCarter, R=7402936927en_US
dc.identifier.scopusauthoridMendis, KN=7004958149en_US

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