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- Publisher Website: 10.1128/IAI.56.5.1158-1161.1988
- Scopus: eid_2-s2.0-0023929117
- PMID: 2833443
- WOS: WOS:A1988N021200024
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Article: Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans
Title | Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans |
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Authors | |
Issue Date | 1988 |
Citation | Infection And Immunity, 1988, v. 56 n. 5, p. 1158-1161 How to Cite? |
Abstract | Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans. |
Persistent Identifier | http://hdl.handle.net/10722/179736 |
ISSN | 2021 Impact Factor: 3.609 2020 SCImago Journal Rankings: 1.508 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Seriwatana, J | en_US |
dc.contributor.author | Echeverria, P | en_US |
dc.contributor.author | Taylor, DN | en_US |
dc.contributor.author | Rasrinaul, L | en_US |
dc.contributor.author | Brown, JE | en_US |
dc.contributor.author | Peiris, JSM | en_US |
dc.contributor.author | Clayton, CL | en_US |
dc.date.accessioned | 2012-12-19T10:04:10Z | - |
dc.date.available | 2012-12-19T10:04:10Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Infection And Immunity, 1988, v. 56 n. 5, p. 1158-1161 | en_US |
dc.identifier.issn | 0019-9567 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179736 | - |
dc.description.abstract | Heat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Infection and Immunity | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Bacterial Toxins - Biosynthesis - Genetics | en_US |
dc.subject.mesh | Biological Assay | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Dna Restriction Enzymes | en_US |
dc.subject.mesh | Enterotoxins - Biosynthesis - Genetics | en_US |
dc.subject.mesh | Escherichia Coli - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Escherichia Coli Proteins | en_US |
dc.subject.mesh | Genes, Bacterial | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Nucleic Acid Hybridization | en_US |
dc.title | Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans | en_US |
dc.type | Article | en_US |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_US |
dc.identifier.authority | Peiris, JSM=rp00410 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1128/IAI.56.5.1158-1161.1988 | - |
dc.identifier.pmid | 2833443 | - |
dc.identifier.scopus | eid_2-s2.0-0023929117 | en_US |
dc.identifier.volume | 56 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 1158 | en_US |
dc.identifier.epage | 1161 | en_US |
dc.identifier.isi | WOS:A1988N021200024 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Seriwatana, J=6603990981 | en_US |
dc.identifier.scopusauthorid | Echeverria, P=35468876700 | en_US |
dc.identifier.scopusauthorid | Taylor, DN=7406867559 | en_US |
dc.identifier.scopusauthorid | Rasrinaul, L=6506672798 | en_US |
dc.identifier.scopusauthorid | Brown, JE=7409447799 | en_US |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_US |
dc.identifier.scopusauthorid | Clayton, CL=7201900754 | en_US |
dc.identifier.issnl | 0019-9567 | - |