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Article: Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans

TitleType II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans
Authors
Issue Date1988
Citation
Infection And Immunity, 1988, v. 56 n. 5, p. 1158-1161 How to Cite?
AbstractHeat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.
Persistent Identifierhttp://hdl.handle.net/10722/179736
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSeriwatana, Jen_US
dc.contributor.authorEcheverria, Pen_US
dc.contributor.authorTaylor, DNen_US
dc.contributor.authorRasrinaul, Len_US
dc.contributor.authorBrown, JEen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorClayton, CLen_US
dc.date.accessioned2012-12-19T10:04:10Z-
dc.date.available2012-12-19T10:04:10Z-
dc.date.issued1988en_US
dc.identifier.citationInfection And Immunity, 1988, v. 56 n. 5, p. 1158-1161en_US
dc.identifier.issn0019-9567en_US
dc.identifier.urihttp://hdl.handle.net/10722/179736-
dc.description.abstractHeat-labile enterotoxin (LT)-producing Escherichia coli strains, as identified by the Y1 adrenal cell assay, were examined with a DNA probe coding for type I and type II LTs. Of 236 LT-producing E. coli isolates, 60% hybridized with LT-I, 17% hybridized with LT-II, and 23% did not hybridize with either probe and no longer produced LT as determined by the Y1 adrenal cell assay. These isolates presumably lost plasmids coding for LT-I during storage. A total of 75% of LT-producing E. coli isolates (27 of 36) from cows, 64% of LT-producing E. coli isolates (7 of 11) from buffalo, 31% of LT-producing E. coli isolates (4 of 13) from beef obtained in markets, and 2% of LT-producing E. coli isolates (3 of 168) from humans contained genes coding for LT-II. Genes coding for LT-II were not found in 50 LT-I-producing and heat-stable enterotoxin-producing E. coli isolates from 11 children with diarrhea and 44 LT-nonproducing and heat-stable enterotoxin-producing E. coli isolates from 12 other children with diarrhea. A total of 9% of LT-II-producing E. coli isolates (3 of 34) from cows and buffalo hybridized with DNA probes for genes coding for verocytotoxin 2 (VT2), and 18% (6 of 34) hybridized with a DNA probe coding for enterohemorrhagic E. coli (EHEC) adhesin fimbriae. E. coli SA-53, the original isolate in which LT-II was found contained genes coding for VT2 and EHEC adhesin fimbriae. Five VT-producing, LT-II-producing E. coli isolates that hybridized with the EHEC probe did not contain DNA sequences coding for VT1 or VT2. LT-II-producing E. coli strains were frequently isolated from cattle and buffalo but were rarely isolated from humans.en_US
dc.languageengen_US
dc.relation.ispartofInfection and Immunityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacterial Toxins - Biosynthesis - Geneticsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna Restriction Enzymesen_US
dc.subject.meshEnterotoxins - Biosynthesis - Geneticsen_US
dc.subject.meshEscherichia Coli - Genetics - Isolation & Purificationen_US
dc.subject.meshEscherichia Coli Proteinsen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshHumansen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.titleType II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humansen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2833443-
dc.identifier.scopuseid_2-s2.0-0023929117en_US
dc.identifier.volume56en_US
dc.identifier.issue5en_US
dc.identifier.spage1158en_US
dc.identifier.epage1161en_US
dc.identifier.isiWOS:A1988N021200024-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSeriwatana, J=6603990981en_US
dc.identifier.scopusauthoridEcheverria, P=35468876700en_US
dc.identifier.scopusauthoridTaylor, DN=7406867559en_US
dc.identifier.scopusauthoridRasrinaul, L=6506672798en_US
dc.identifier.scopusauthoridBrown, JE=7409447799en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridClayton, CL=7201900754en_US

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