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Article: Immunoelectron microscopy of Schuffner's dots in Plasmodium vivax-infected human erythrocytes

TitleImmunoelectron microscopy of Schuffner's dots in Plasmodium vivax-infected human erythrocytes
Authors
Issue Date1988
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1988, v. 131 n. 1, p. 48-52 How to Cite?
AbstractPlasmodium vivax induces morphologic alterations in infected host erythrocytes that are visible by light microscopy in Romanovsky-stained blood smears as multiple brick-red dots. These morphologic changes, referred to as Schuffner's dots, are important in the identification of this species of malarial parasite and have been associated by electron microscopy with caveola-vesicle complexes along the erythrocyte plasmalemma. We have produced a monoclonal antibody (MAb A 20) that identifies an antigen in Plasmodium vivax-infected erythrocytes that is associated with the caveola-vesicle complexes of the parasitized host cell. This monoclonal antibody reacts with air-dried P. vivax-infected erythrocytes to produce a pattern by the indirect immunofluorescence test (IFT) that is evocative of Schuffner's dots. Immunoelectron microscopy of P. vivax-infected human erythrocytes using MAb A 20 confirmed the location of this antigen within vesicles of caveola-vesicle complexes. On Western blots MAb A 20 recognized four polypeptides of 54, 64, 72, and 86 kd. MAb A 20 reacted by IFT with 90% of Sri Lankan isolates and with a Brazilian P. vixax isolate, which indicates that the epitope identified by this monoclonal is conserved.
Persistent Identifierhttp://hdl.handle.net/10722/179735
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorUdagama, PVen_US
dc.contributor.authorAtkinson, CTen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorDavid, PHen_US
dc.contributor.authorMendis, KNen_US
dc.contributor.authorAikawa, Men_US
dc.date.accessioned2012-12-19T10:04:10Z-
dc.date.available2012-12-19T10:04:10Z-
dc.date.issued1988en_US
dc.identifier.citationAmerican Journal Of Pathology, 1988, v. 131 n. 1, p. 48-52en_US
dc.identifier.issn0002-9440en_US
dc.identifier.urihttp://hdl.handle.net/10722/179735-
dc.description.abstractPlasmodium vivax induces morphologic alterations in infected host erythrocytes that are visible by light microscopy in Romanovsky-stained blood smears as multiple brick-red dots. These morphologic changes, referred to as Schuffner's dots, are important in the identification of this species of malarial parasite and have been associated by electron microscopy with caveola-vesicle complexes along the erythrocyte plasmalemma. We have produced a monoclonal antibody (MAb A 20) that identifies an antigen in Plasmodium vivax-infected erythrocytes that is associated with the caveola-vesicle complexes of the parasitized host cell. This monoclonal antibody reacts with air-dried P. vivax-infected erythrocytes to produce a pattern by the indirect immunofluorescence test (IFT) that is evocative of Schuffner's dots. Immunoelectron microscopy of P. vivax-infected human erythrocytes using MAb A 20 confirmed the location of this antigen within vesicles of caveola-vesicle complexes. On Western blots MAb A 20 recognized four polypeptides of 54, 64, 72, and 86 kd. MAb A 20 reacted by IFT with 90% of Sri Lankan isolates and with a Brazilian P. vixax isolate, which indicates that the epitope identified by this monoclonal is conserved.en_US
dc.languageengen_US
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_US
dc.relation.ispartofAmerican Journal of Pathologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshErythrocytes - Parasitology - Ultrastructureen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria - Blooden_US
dc.subject.meshMicroscopy, Electron - Methodsen_US
dc.subject.meshPlasmodium Vivax - Isolation & Purificationen_US
dc.titleImmunoelectron microscopy of Schuffner's dots in Plasmodium vivax-infected human erythrocytesen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3281470-
dc.identifier.scopuseid_2-s2.0-0023818298en_US
dc.identifier.volume131en_US
dc.identifier.issue1en_US
dc.identifier.spage48en_US
dc.identifier.epage52en_US
dc.identifier.isiWOS:A1988M930100007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridUdagama, PV=6505767945en_US
dc.identifier.scopusauthoridAtkinson, CT=7201407887en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridDavid, PH=7201509648en_US
dc.identifier.scopusauthoridMendis, KN=7004958149en_US
dc.identifier.scopusauthoridAikawa, M=7201578834en_US

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