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Article: New applications of low-C(o)t DNA as a DNA fingerprint probe

TitleNew applications of low-C(o)t DNA as a DNA fingerprint probe
Authors
Issue Date1999
Citation
Electrophoresis, 1999, v. 20 n. 8, p. 1762-1767 How to Cite?
AbstractNew applications of low-C(o)t DNA are reported as probes for genetic identification and genome characterization. These fast and intermediately reannealing fractions have sometimes either been discarded in genomic library construction to enhance the probability of finding single copy genes, or they are used as resources for identifying individual repetitive sequences. In addition, they are used as blockers to enhance hybridization signals. C(o)t- 1 DNA serves as a probe for DNA fingerprinting of human yeast artificial chromosomes. We have isolated low-C(o)t DNA from bacteria, fungus, plant, mussel, chicken, rat and fish from the sheared genomic DNA of the respective species. Low-C(o)t DNA is labeled to generate DNA fingerprints and for in situ hybridization. Individual specific DNA fingerprint profiles are observed and species-specific DNA fragments can be identified in bacteria, fungus, plants (Ginseng and Amaranthus) and mussel. When low-C(o)t DNA probes from rat, chicken and fish were employed, only smear profiles and no distinct DNA banding patterns were evident. In these species individual clones can be used as a probe for DNA fingerprinting containing repetitive sequences after subcloning. The advantage of this approach is to quickly develop useful probe for DNA fingerprinting for genetic identification and analysis without sequencing knowledge a priori. This represents an innovative approach to the use of these repetitive components of the genome.
Persistent Identifierhttp://hdl.handle.net/10722/179580
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:59:58Z-
dc.date.available2012-12-19T09:59:58Z-
dc.date.issued1999en_US
dc.identifier.citationElectrophoresis, 1999, v. 20 n. 8, p. 1762-1767en_US
dc.identifier.issn0173-0835en_US
dc.identifier.urihttp://hdl.handle.net/10722/179580-
dc.description.abstractNew applications of low-C(o)t DNA are reported as probes for genetic identification and genome characterization. These fast and intermediately reannealing fractions have sometimes either been discarded in genomic library construction to enhance the probability of finding single copy genes, or they are used as resources for identifying individual repetitive sequences. In addition, they are used as blockers to enhance hybridization signals. C(o)t- 1 DNA serves as a probe for DNA fingerprinting of human yeast artificial chromosomes. We have isolated low-C(o)t DNA from bacteria, fungus, plant, mussel, chicken, rat and fish from the sheared genomic DNA of the respective species. Low-C(o)t DNA is labeled to generate DNA fingerprints and for in situ hybridization. Individual specific DNA fingerprint profiles are observed and species-specific DNA fragments can be identified in bacteria, fungus, plants (Ginseng and Amaranthus) and mussel. When low-C(o)t DNA probes from rat, chicken and fish were employed, only smear profiles and no distinct DNA banding patterns were evident. In these species individual clones can be used as a probe for DNA fingerprinting containing repetitive sequences after subcloning. The advantage of this approach is to quickly develop useful probe for DNA fingerprinting for genetic identification and analysis without sequencing knowledge a priori. This represents an innovative approach to the use of these repetitive components of the genome.en_US
dc.languageengen_US
dc.relation.ispartofElectrophoresisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacteria - Geneticsen_US
dc.subject.meshBivalvia - Geneticsen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshDna Probesen_US
dc.subject.meshFusarium - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshPanax - Geneticsen_US
dc.subject.meshPlants, Medicinalen_US
dc.subject.meshRatsen_US
dc.titleNew applications of low-C(o)t DNA as a DNA fingerprint probeen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1522-2683(19990101)20:8<1762::AID-ELPS1762>3.0.CO;2-Xen_US
dc.identifier.pmid10435445-
dc.identifier.scopuseid_2-s2.0-0033064930en_US
dc.identifier.hkuros42322-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033064930&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue8en_US
dc.identifier.spage1762en_US
dc.identifier.epage1767en_US
dc.identifier.isiWOS:000081484900017-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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