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Article: Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities

TitleProtein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities
Authors
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal of Agricultural and Food Chemistry, 2011, v. 59 n. 18, p. 10232-10238 How to Cite?
AbstractA 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC 50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC 50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC 50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC 50 = 50 and 90 μM, respectively. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/179460
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYe, XJen_US
dc.contributor.authorNg, TBen_US
dc.contributor.authorWu, ZJen_US
dc.contributor.authorXie, LHen_US
dc.contributor.authorFang, EFen_US
dc.contributor.authorWong, JHen_US
dc.contributor.authorPan, WLen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorZhang, YBen_US
dc.date.accessioned2012-12-19T09:56:46Z-
dc.date.available2012-12-19T09:56:46Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2011, v. 59 n. 18, p. 10232-10238en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/179460-
dc.description.abstractA 30 kDa antifungal protein was purified from red cabbage (Brassica oleracea) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC 50 = 5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC 50 = 96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC 50 = 53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC 50 = 50 and 90 μM, respectively. © 2011 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject.meshAnti-Bacterial Agents - Pharmacologyen_US
dc.subject.meshAntifungal Agents - Pharmacologyen_US
dc.subject.meshAntineoplastic Agents, Phytogenic - Pharmacologyen_US
dc.subject.meshBrassica - Chemistryen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshFungicides, Industrial - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMycelium - Drug Effects - Growth & Developmenten_US
dc.subject.meshPlant Proteins - Isolation & Purification - Pharmacologyen_US
dc.subject.meshPseudomonas Aeruginosa - Drug Effectsen_US
dc.subject.meshSeeds - Chemistryen_US
dc.titleProtein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activitiesen_US
dc.typeArticleen_US
dc.identifier.emailZhang, YB: ybzhang@hku.hken_US
dc.identifier.authorityZhang, YB=rp01410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf201874jen_US
dc.identifier.pmid21830763-
dc.identifier.scopuseid_2-s2.0-80053124566en_US
dc.identifier.hkuros202966-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053124566&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume59en_US
dc.identifier.issue18en_US
dc.identifier.spage10232en_US
dc.identifier.epage10238en_US
dc.identifier.eissn1520-5118-
dc.identifier.isiWOS:000295059100063-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYe, XJ=7401899033en_US
dc.identifier.scopusauthoridNg, TB=35311803300en_US
dc.identifier.scopusauthoridWu, ZJ=8454392100en_US
dc.identifier.scopusauthoridXie, LH=8281904800en_US
dc.identifier.scopusauthoridFang, EF=35386218400en_US
dc.identifier.scopusauthoridWong, JH=34874358200en_US
dc.identifier.scopusauthoridPan, WL=35234566600en_US
dc.identifier.scopusauthoridWing, SSC=52664637600en_US
dc.identifier.scopusauthoridZhang, YB=23483121900en_US

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