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Article: Simultaneous Analysis of Lipid-Soluble Constituents of Erxian Decoction by GC-MS

TitleSimultaneous Analysis of Lipid-Soluble Constituents of Erxian Decoction by GC-MS
Authors
KeywordsChinese Medicine Formula
Erxian Decoction
Gas Chromatography-Mass Spectrometry
Issue Date2009
PublisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographia
Citation
Chromatographia, 2009, v. 70 n. 7-8, p. 1171-1177 How to Cite?
AbstractErxian Decoction (EXD), a traditional Chinese medicine formula mainly composed of six Chinese herbs, was originally developed for menopausal syndromes and had been practiced since the 1950s in China. Previous studies only focused on the water-soluble compounds involved in EXD by LC or TLC. This study analyzed the whole profile of the volatile constituents contained in EXD to supplement its quality evaluation method. Several EXD samples were extracted with chloroform and ethyl acetate, respectively, to get the lipid-soluble chloroform and ethyl acetate fractions and compared their gas chromatographic profiles by GC-MS. The EXD samples were hydrolyzed with hydrochloric acid in a water-bath at 100 °C, neutralized with 40% NaOH, and finally extracted with ethyl acetate and chloroform for the quantification of the total sarsasapogenins contained in EXD. A total of 56 compounds belonging to a variety of natural product categories such as aromatic phenols, terpenes, fatty acids, ketones, esters, and aldehydes, etc. were identified from the chloroform and ethyl acetate extracts by using the online EI-MS characterization. The GC-MS method showed a linear response for sarsasapogenin quantification with r = 0.994. The intra-day and inter-day variations of precision and accuracy of the assay were less than 5%. This developed GC-MS method could thus be successfully applied for the identification of lipid-soluble constituents derived from EXD, and also for the accurate quantification of the total sarsasapogenins contained in the acid hydrolyzed EXD samples. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/179453
ISSN
2015 Impact Factor: 1.332
2015 SCImago Journal Rankings: 0.480
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHu, YMen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorZhang, XQen_US
dc.contributor.authorTong, Yen_US
dc.date.accessioned2012-12-19T09:56:42Z-
dc.date.available2012-12-19T09:56:42Z-
dc.date.issued2009en_US
dc.identifier.citationChromatographia, 2009, v. 70 n. 7-8, p. 1171-1177en_US
dc.identifier.issn0009-5893en_US
dc.identifier.urihttp://hdl.handle.net/10722/179453-
dc.description.abstractErxian Decoction (EXD), a traditional Chinese medicine formula mainly composed of six Chinese herbs, was originally developed for menopausal syndromes and had been practiced since the 1950s in China. Previous studies only focused on the water-soluble compounds involved in EXD by LC or TLC. This study analyzed the whole profile of the volatile constituents contained in EXD to supplement its quality evaluation method. Several EXD samples were extracted with chloroform and ethyl acetate, respectively, to get the lipid-soluble chloroform and ethyl acetate fractions and compared their gas chromatographic profiles by GC-MS. The EXD samples were hydrolyzed with hydrochloric acid in a water-bath at 100 °C, neutralized with 40% NaOH, and finally extracted with ethyl acetate and chloroform for the quantification of the total sarsasapogenins contained in EXD. A total of 56 compounds belonging to a variety of natural product categories such as aromatic phenols, terpenes, fatty acids, ketones, esters, and aldehydes, etc. were identified from the chloroform and ethyl acetate extracts by using the online EI-MS characterization. The GC-MS method showed a linear response for sarsasapogenin quantification with r = 0.994. The intra-day and inter-day variations of precision and accuracy of the assay were less than 5%. This developed GC-MS method could thus be successfully applied for the identification of lipid-soluble constituents derived from EXD, and also for the accurate quantification of the total sarsasapogenins contained in the acid hydrolyzed EXD samples. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH.en_US
dc.languageengen_US
dc.publisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographiaen_US
dc.relation.ispartofChromatographiaen_US
dc.subjectChinese Medicine Formulaen_US
dc.subjectErxian Decoctionen_US
dc.subjectGas Chromatography-Mass Spectrometryen_US
dc.titleSimultaneous Analysis of Lipid-Soluble Constituents of Erxian Decoction by GC-MSen_US
dc.typeArticleen_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1365/s10337-009-1295-5en_US
dc.identifier.scopuseid_2-s2.0-79551515019en_US
dc.identifier.hkuros160699-
dc.identifier.volume70-
dc.identifier.issue7-8-
dc.identifier.spage1en_US
dc.identifier.spage1171-
dc.identifier.epage7en_US
dc.identifier.epage1177-
dc.identifier.isiWOS:000271069400021-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridHu, YM=7407116890en_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridZhang, XQ=25629138600en_US
dc.identifier.scopusauthoridTong, Y=9045384000en_US
dc.identifier.citeulike5815573-

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