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Article: Wheat germ lectin induces G 2/M arrest in mouse L929 fibroblasts

TitleWheat germ lectin induces G 2/M arrest in mouse L929 fibroblasts
Authors
KeywordsCyclin Kinase Inhibitor
Growth Arrest
L929 Fibroblast Cell Line
Wheat Germ Lectin
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2004, v. 91 n. 6, p. 1159-1173 How to Cite?
AbstractWheat germ lectin (WGA) is a cytotoxic lectin for many cell lines [Wang et al., 2000], but its underlying mechanism is not clear. In this report, we found that incubation of synchronized mouse L929 fibroblasts with WGA resulted in a dose-dependent reduction of intracellular incorporation of 3H- thymidine and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)-conversion activity (IC 50 ≅ 0.4 μM). Fluorescein-conjugated WGA was demonstrated to transport from the cell surface into the paranuclear region of cultured L929 cells within 30 min, and subsequently evoked lipid peroxidation of plasma membrane and vacuolation in the cytoplasm of these cells. Studies with tritiated thymidine incorporation, immunofluorescence microscopy, immunoblotting analysis and flow cytometry revealed that WGA inhibited cell cycle progression after one replication, resulting in G 2/M arrest and alteration of cell cycle regulatory proteins, particularly activation of p21 Cip1/WAF1 and suppression of cyclin B and cdc 2. Although there was an increase of cytosolic caspase 3 and bax protein expression, no apoptotic bodies were observed by both fluorescence and transmission electron microscopy. These results suggest that WGA arrested L929 proliferation after one cell cycle in the G 2/M phase through activation of the p21 Cip1/WAF1 and suppression of Cyclin B-Cdc2. © 2004 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179446
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, WKen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorHo, JCKen_US
dc.contributor.authorLiu, BPLen_US
dc.contributor.authorYu, MCen_US
dc.date.accessioned2012-12-19T09:56:39Z-
dc.date.available2012-12-19T09:56:39Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Cellular Biochemistry, 2004, v. 91 n. 6, p. 1159-1173en_US
dc.identifier.issn0730-2312en_US
dc.identifier.urihttp://hdl.handle.net/10722/179446-
dc.description.abstractWheat germ lectin (WGA) is a cytotoxic lectin for many cell lines [Wang et al., 2000], but its underlying mechanism is not clear. In this report, we found that incubation of synchronized mouse L929 fibroblasts with WGA resulted in a dose-dependent reduction of intracellular incorporation of 3H- thymidine and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)-conversion activity (IC 50 ≅ 0.4 μM). Fluorescein-conjugated WGA was demonstrated to transport from the cell surface into the paranuclear region of cultured L929 cells within 30 min, and subsequently evoked lipid peroxidation of plasma membrane and vacuolation in the cytoplasm of these cells. Studies with tritiated thymidine incorporation, immunofluorescence microscopy, immunoblotting analysis and flow cytometry revealed that WGA inhibited cell cycle progression after one replication, resulting in G 2/M arrest and alteration of cell cycle regulatory proteins, particularly activation of p21 Cip1/WAF1 and suppression of cyclin B and cdc 2. Although there was an increase of cytosolic caspase 3 and bax protein expression, no apoptotic bodies were observed by both fluorescence and transmission electron microscopy. These results suggest that WGA arrested L929 proliferation after one cell cycle in the G 2/M phase through activation of the p21 Cip1/WAF1 and suppression of Cyclin B-Cdc2. © 2004 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_US
dc.relation.ispartofJournal of Cellular Biochemistryen_US
dc.subjectCyclin Kinase Inhibitoren_US
dc.subjectGrowth Arresten_US
dc.subjectL929 Fibroblast Cell Lineen_US
dc.subjectWheat Germ Lectinen_US
dc.titleWheat germ lectin induces G 2/M arrest in mouse L929 fibroblastsen_US
dc.typeArticleen_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcb.10755en_US
dc.identifier.pmid15048871-
dc.identifier.scopuseid_2-s2.0-4444251768en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4444251768&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume91en_US
dc.identifier.issue6en_US
dc.identifier.spage1159en_US
dc.identifier.epage1173en_US
dc.identifier.isiWOS:000220701000006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiu, WK=13204573100en_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridHo, JCK=7402649716en_US
dc.identifier.scopusauthoridLiu, BPL=7408693125en_US
dc.identifier.scopusauthoridYu, MC=36800580200en_US

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