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Article: Volvariella volvacea lectin activates mouse T lymphocytes by a calcium dependent pathway

TitleVolvariella volvacea lectin activates mouse T lymphocytes by a calcium dependent pathway
Authors
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2004, v. 92 n. 6, p. 1193-1202 How to Cite?
AbstractThe immunomodulatory lectin, Volvariella volvacea lectin (VVL), isolated from the edible mushroom, Volvariella volvacea, has been shown to stimulate the expression of Th1 cytokines and the proliferative activity of mouse splenocytes (She et al. [1998]: Biochem Biophys Res Comm 247:106-111). In order to elucidate the mechanisms underlying these activities, we conducted a kinetic analysis of the early and late activation markers in mouse T lymphocytes: (1) flow cytometric analysis of calcium influx, (2) induction of activation molecules (CD25 and CD69), (3) expression and DNA-binding activity of the nuclear factor of activated T cells (NFAT), NFκB, and activation protein-1 (AP-1), (4) translational production of cytokines (interleukin-2 (IL-2) and interferon-γ (IFNγ)), and (5) cell proliferation by expression of proliferating cell nuclear antigen (PCNA) and tritiated thymidine incorporation. All results showed that VVL induced a rapid expression of CD69, CD25, NFAT, IL-2, and PCNA in a dose- and time-dependent manner, leading to lymphocyte proliferation. These effects brought about by VVL were more potent than those stimulated by equimolar concentrations of mitogenic lectin, concanavalin A (Con A). Cell activation and proliferation were mediated through a calcium-dependent pathway as demonstrated by a VVL-induced increase of intracellular calcium influx, and a proliferation inhibition by the Ca-dependent phosphatase calcineurin blocker-cyclosporin A (CsA). Taken all data together, VVL is a lectin which activates lymphocyte through successive calcium influx, nuclear localization of NFAT transcription factor, induction of activation markers, CD25 and CD69, intracellular cytokine production, and cell proliferation. © 2004 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179423
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSze, SCWen_US
dc.contributor.authorHo, JCKen_US
dc.contributor.authorLiu, WKen_US
dc.date.accessioned2012-12-19T09:56:21Z-
dc.date.available2012-12-19T09:56:21Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Cellular Biochemistry, 2004, v. 92 n. 6, p. 1193-1202en_US
dc.identifier.issn0730-2312en_US
dc.identifier.urihttp://hdl.handle.net/10722/179423-
dc.description.abstractThe immunomodulatory lectin, Volvariella volvacea lectin (VVL), isolated from the edible mushroom, Volvariella volvacea, has been shown to stimulate the expression of Th1 cytokines and the proliferative activity of mouse splenocytes (She et al. [1998]: Biochem Biophys Res Comm 247:106-111). In order to elucidate the mechanisms underlying these activities, we conducted a kinetic analysis of the early and late activation markers in mouse T lymphocytes: (1) flow cytometric analysis of calcium influx, (2) induction of activation molecules (CD25 and CD69), (3) expression and DNA-binding activity of the nuclear factor of activated T cells (NFAT), NFκB, and activation protein-1 (AP-1), (4) translational production of cytokines (interleukin-2 (IL-2) and interferon-γ (IFNγ)), and (5) cell proliferation by expression of proliferating cell nuclear antigen (PCNA) and tritiated thymidine incorporation. All results showed that VVL induced a rapid expression of CD69, CD25, NFAT, IL-2, and PCNA in a dose- and time-dependent manner, leading to lymphocyte proliferation. These effects brought about by VVL were more potent than those stimulated by equimolar concentrations of mitogenic lectin, concanavalin A (Con A). Cell activation and proliferation were mediated through a calcium-dependent pathway as demonstrated by a VVL-induced increase of intracellular calcium influx, and a proliferation inhibition by the Ca-dependent phosphatase calcineurin blocker-cyclosporin A (CsA). Taken all data together, VVL is a lectin which activates lymphocyte through successive calcium influx, nuclear localization of NFAT transcription factor, induction of activation markers, CD25 and CD69, intracellular cytokine production, and cell proliferation. © 2004 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_US
dc.relation.ispartofJournal of Cellular Biochemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBasidiomycota - Chemistryen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshDna - Metabolismen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshElectrophoretic Mobility Shift Assayen_US
dc.subject.meshLectins - Pharmacologyen_US
dc.subject.meshLymphocyte Activation - Drug Effectsen_US
dc.subject.meshMiceen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshT-Lymphocytes - Drug Effects - Immunologyen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.titleVolvariella volvacea lectin activates mouse T lymphocytes by a calcium dependent pathwayen_US
dc.typeArticleen_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcb.20153en_US
dc.identifier.pmid15258902-
dc.identifier.scopuseid_2-s2.0-16644397688en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16644397688&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume92en_US
dc.identifier.issue6en_US
dc.identifier.spage1193en_US
dc.identifier.epage1202en_US
dc.identifier.isiWOS:000223157200010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridHo, JCK=7402649716en_US
dc.identifier.scopusauthoridLiu, WK=13204573100en_US

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