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Article: Mitogenic activity of edible mushroom lectins

TitleMitogenic activity of edible mushroom lectins
Authors
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagen
Citation
Biochimica Et Biophysica Acta - General Subjects, 2004, v. 1671 n. 1-3, p. 9-17 How to Cite?
AbstractA special group of lectins were isolated from three popular Asian edible mushrooms: Volvariella volvacea, Pleurotus flabellatus and Hericium erinacium, and their mitogenic activities towards mouse T cells were compared to the extensively investigated Agaricus bisporus lectin (ABL) and the Jack bean lectin, Concanavalin A (Con A). Among the four mushroom lectins tested, V. volvacea lectin (VVL) exhibited strong mitogenic activity as demonstrated by 3H-thymidine incorporation, which was at least 10-fold more effective than that of Con A, and the other mushroom lectins did not exhibit any proliferative activity. Treatment with VVL and ABL resulted in activation of the protein tyrosine kinase, p56lck, and expression of early activation markers, CD69 and CD25, but only VVL induced intracellular calcium influx while ABL triggered cell death. The calcium influx was sensitive to calcium channel antagonists such as nifedipine and verapamil. The P. flabellatus lectin (PFL) and H. erinacium lectin (HEL) did not stimulate p56lck expression and cell proliferation. Neither of these lectins interfered with Con A-mediated lymphocyte proliferation, which further indicated that both PFL and HEL were non-mitogenic. Taken all results together, VVL induced mitogenesis through T cell receptors and the subsequent calcium signaling pathway. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179421
ISSN
2015 Impact Factor: 5.083
2015 SCImago Journal Rankings: 2.128
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, JCKen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorShen, WZen_US
dc.contributor.authorLiu, WKen_US
dc.date.accessioned2012-12-19T09:56:20Z-
dc.date.available2012-12-19T09:56:20Z-
dc.date.issued2004en_US
dc.identifier.citationBiochimica Et Biophysica Acta - General Subjects, 2004, v. 1671 n. 1-3, p. 9-17en_US
dc.identifier.issn0304-4165en_US
dc.identifier.urihttp://hdl.handle.net/10722/179421-
dc.description.abstractA special group of lectins were isolated from three popular Asian edible mushrooms: Volvariella volvacea, Pleurotus flabellatus and Hericium erinacium, and their mitogenic activities towards mouse T cells were compared to the extensively investigated Agaricus bisporus lectin (ABL) and the Jack bean lectin, Concanavalin A (Con A). Among the four mushroom lectins tested, V. volvacea lectin (VVL) exhibited strong mitogenic activity as demonstrated by 3H-thymidine incorporation, which was at least 10-fold more effective than that of Con A, and the other mushroom lectins did not exhibit any proliferative activity. Treatment with VVL and ABL resulted in activation of the protein tyrosine kinase, p56lck, and expression of early activation markers, CD69 and CD25, but only VVL induced intracellular calcium influx while ABL triggered cell death. The calcium influx was sensitive to calcium channel antagonists such as nifedipine and verapamil. The P. flabellatus lectin (PFL) and H. erinacium lectin (HEL) did not stimulate p56lck expression and cell proliferation. Neither of these lectins interfered with Con A-mediated lymphocyte proliferation, which further indicated that both PFL and HEL were non-mitogenic. Taken all results together, VVL induced mitogenesis through T cell receptors and the subsequent calcium signaling pathway. © 2004 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagenen_US
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjectsen_US
dc.subject.meshAgaricales - Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Metabolismen_US
dc.subject.meshAntigens, Differentiation, T-Lymphocyte - Metabolismen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshLectins - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshLectins, C-Typeen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshLymphocyte Specific Protein Tyrosine Kinase P56(Lck) - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshReceptors, Interleukin-2 - Metabolismen_US
dc.subject.meshSpleen - Cytologyen_US
dc.subject.meshT-Lymphocytes - Cytology - Drug Effects - Metabolismen_US
dc.titleMitogenic activity of edible mushroom lectinsen_US
dc.typeArticleen_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbagen.2003.12.009en_US
dc.identifier.pmid15026140-
dc.identifier.scopuseid_2-s2.0-1542298279en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542298279&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1671en_US
dc.identifier.issue1-3en_US
dc.identifier.spage9en_US
dc.identifier.epage17en_US
dc.identifier.isiWOS:000220457300002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridHo, JCK=7402649716en_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridShen, WZ=36778235700en_US
dc.identifier.scopusauthoridLiu, WK=13204573100en_US

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