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Article: Study on preventive effect of buyang huanwu decoction on cardiomyocyte apoptosis induced by hypoxia-reoxygenation in rats

TitleStudy on preventive effect of buyang huanwu decoction on cardiomyocyte apoptosis induced by hypoxia-reoxygenation in rats
Authors
Issue Date2002
PublisherZhongguo Zhong-Xiyi Jiehe Zazhi. The Journal's web site is located at http://zxyjh.periodicals.net.cn/
Citation
Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi = Chinese Journal Of Integrated Traditional And Western Medicine / Zhongguo Zhong Xi Yi Jie He Xue Hui, Zhongguo Zhong Yi Yan Jiu Yuan Zhu Ban, 2002, v. 22 n. 7, p. 522-524 How to Cite?
AbstractOBJECTIVE: To observe the preventive effect of rats' serum containing Buyang Huanwu Decoction (BYHWD) on cultured cardiomyocyte apoptosis of neonatal rat induced by means of 24 hrs hypoxia and 4 hrs reoxygenation, and to investigate its mechanism concerned with nitric oxide (NO). METHODS: Myocyte apoptosis was detected by flow cytometry and ELISA with Annexin V-PI double labeled method. The lactate dehydrogenase (LDH) releasing level was measured with ultraviolet spectrophotometer. The NO concentration was determined by modified Yu method and the concentration of thiobarbituric acid response substance (TBARS) was tested by Ohkawa method. RESULTS: BYHWD contained rats' serum could significantly prevent cardiomyocyte from apoptosis induced by hypoxia and reoxygenation. After hypoxia-reoxygenation, the NO, LDH and TBARS levels in the supernatant of cultured liquid treated with BYHWD were significantly lower than those in non-treated cultured liquid, the effect of BYHWD was dose-dependent. CONCLUSION: BYHWD can prevent cardiomyocytes from apoptosis induced by hypoxia and reoxygenation, its mechanism might be related with oxygen free radical and NO scavenging produced during the hypoxia-reoxygenation process.
Persistent Identifierhttp://hdl.handle.net/10722/179416
ISSN
2015 SCImago Journal Rankings: 0.140

 

DC FieldValueLanguage
dc.contributor.authorTong, Len_US
dc.contributor.authorShen, JGen_US
dc.contributor.authorQiu, XSen_US
dc.date.accessioned2012-12-19T09:56:18Z-
dc.date.available2012-12-19T09:56:18Z-
dc.date.issued2002en_US
dc.identifier.citationZhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi = Chinese Journal Of Integrated Traditional And Western Medicine / Zhongguo Zhong Xi Yi Jie He Xue Hui, Zhongguo Zhong Yi Yan Jiu Yuan Zhu Ban, 2002, v. 22 n. 7, p. 522-524en_US
dc.identifier.issn1003-5370en_US
dc.identifier.urihttp://hdl.handle.net/10722/179416-
dc.description.abstractOBJECTIVE: To observe the preventive effect of rats' serum containing Buyang Huanwu Decoction (BYHWD) on cultured cardiomyocyte apoptosis of neonatal rat induced by means of 24 hrs hypoxia and 4 hrs reoxygenation, and to investigate its mechanism concerned with nitric oxide (NO). METHODS: Myocyte apoptosis was detected by flow cytometry and ELISA with Annexin V-PI double labeled method. The lactate dehydrogenase (LDH) releasing level was measured with ultraviolet spectrophotometer. The NO concentration was determined by modified Yu method and the concentration of thiobarbituric acid response substance (TBARS) was tested by Ohkawa method. RESULTS: BYHWD contained rats' serum could significantly prevent cardiomyocyte from apoptosis induced by hypoxia and reoxygenation. After hypoxia-reoxygenation, the NO, LDH and TBARS levels in the supernatant of cultured liquid treated with BYHWD were significantly lower than those in non-treated cultured liquid, the effect of BYHWD was dose-dependent. CONCLUSION: BYHWD can prevent cardiomyocytes from apoptosis induced by hypoxia and reoxygenation, its mechanism might be related with oxygen free radical and NO scavenging produced during the hypoxia-reoxygenation process.en_US
dc.languageengen_US
dc.publisherZhongguo Zhong-Xiyi Jiehe Zazhi. The Journal's web site is located at http://zxyjh.periodicals.net.cn/en_US
dc.relation.ispartofZhongguo zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu banen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCell Hypoxiaen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacologyen_US
dc.subject.meshMyocytes, Cardiac - Cytology - Drug Effectsen_US
dc.subject.meshNitric Oxide - Metabolismen_US
dc.subject.meshOxygen - Pharmacologyen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleStudy on preventive effect of buyang huanwu decoction on cardiomyocyte apoptosis induced by hypoxia-reoxygenation in ratsen_US
dc.typeArticleen_US
dc.identifier.emailShen, JG: shenjg@hku.hken_US
dc.identifier.authorityShen, JG=rp00487en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12592688-
dc.identifier.scopuseid_2-s2.0-0038205289en_US
dc.identifier.volume22en_US
dc.identifier.issue7en_US
dc.identifier.spage522en_US
dc.identifier.epage524en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridTong, L=7201891650en_US
dc.identifier.scopusauthoridShen, JG=7404929947en_US
dc.identifier.scopusauthoridQiu, XS=12040272400en_US

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