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Article: Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium

TitleKlebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium
Authors
Issue Date2004
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal Of Systematic And Evolutionary Microbiology, 2004, v. 54 n. 6, p. 2131-2136 How to Cite?
AbstractCells of strain LX3T, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 °C, unable to produce gas from lactose at 45 °C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56.4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Kiebsiella pneumoniae, there being 99.3 and 97.5% similarity in 16S rRNA and rpoB gene sequences, respectively. DNA-DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Kiebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA-DNA reassociation data. Therefore, it is proposed that strain LX3T (=DSM 16265T =JCM 12419T) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov. © 2004 IUMS.
Persistent Identifierhttp://hdl.handle.net/10722/179343
ISSN
2015 Impact Factor: 2.439
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Xen_US
dc.contributor.authorZhang, Den_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorMa, Jen_US
dc.contributor.authorDong, Yen_US
dc.contributor.authorZhang, Len_US
dc.date.accessioned2012-12-19T09:54:20Z-
dc.date.available2012-12-19T09:54:20Z-
dc.date.issued2004en_US
dc.identifier.citationInternational Journal Of Systematic And Evolutionary Microbiology, 2004, v. 54 n. 6, p. 2131-2136en_US
dc.identifier.issn1466-5026en_US
dc.identifier.urihttp://hdl.handle.net/10722/179343-
dc.description.abstractCells of strain LX3T, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 °C, unable to produce gas from lactose at 45 °C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56.4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Kiebsiella pneumoniae, there being 99.3 and 97.5% similarity in 16S rRNA and rpoB gene sequences, respectively. DNA-DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Kiebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA-DNA reassociation data. Therefore, it is proposed that strain LX3T (=DSM 16265T =JCM 12419T) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov. © 2004 IUMS.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.orgen_US
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiologyen_US
dc.subject.meshAnaerobiosisen_US
dc.subject.meshBacterial Capsules - Biosynthesisen_US
dc.subject.meshBase Compositionen_US
dc.subject.meshDna, Bacterial - Chemistry - Isolation & Purificationen_US
dc.subject.meshDna, Ribosomal - Chemistry - Isolation & Purificationen_US
dc.subject.meshDna-Directed Rna Polymerases - Geneticsen_US
dc.subject.meshGenes, Rrnaen_US
dc.subject.meshGentian Violeten_US
dc.subject.meshGlucose - Metabolismen_US
dc.subject.meshIndoles - Metabolismen_US
dc.subject.meshIsomaltose - Analogs & Derivatives - Biosynthesisen_US
dc.subject.meshKlebsiella - Classification - Cytology - Isolation & Purification - Physiologyen_US
dc.subject.meshLactose - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMovementen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPhenazinesen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRna, Bacterial - Geneticsen_US
dc.subject.meshRna, Ribosomal, 16S - Geneticsen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSoil Microbiologyen_US
dc.subject.meshSpores, Bacterial - Cytologyen_US
dc.subject.meshSucrose - Metabolismen_US
dc.subject.meshTemperatureen_US
dc.titleKlebsiella singaporensis sp. nov., a novel isomaltulose-producing bacteriumen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/ijs.0.02690-0en_US
dc.identifier.pmid15545446-
dc.identifier.scopuseid_2-s2.0-9744242714en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9744242714&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue6en_US
dc.identifier.spage2131en_US
dc.identifier.epage2136en_US
dc.identifier.isiWOS:000225366000039-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLi, X=8869956800en_US
dc.identifier.scopusauthoridZhang, D=7405355717en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridMa, J=7406200410en_US
dc.identifier.scopusauthoridDong, Y=7403390435en_US
dc.identifier.scopusauthoridZhang, L=8599921900en_US

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