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Article: Evidence for a novel intrapituitary autocrine/paracrine feedback loop regulating growth hormone synthesis and secretion in grass carp pituitary cells by functional interactions between gonadotrophs and somatotrophs

TitleEvidence for a novel intrapituitary autocrine/paracrine feedback loop regulating growth hormone synthesis and secretion in grass carp pituitary cells by functional interactions between gonadotrophs and somatotrophs
Authors
Issue Date2004
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2004, v. 145 n. 12, p. 5548-5559 How to Cite?
AbstractGonadotropin (GTH) and GH released from the pituitary are known to interact at multiple levels to modulate the functions of the gonadotrophic and somatotrophic axes. However, their interactions at the pituitary level have not been fully characterized. In this study, autocrine/paracrine regulation of GH synthesis and secretion by local interactions between gonadotrophs and somatotrophs was examined using grass carp pituitary cells as a cell model. Exogenous GTH and GH induced GH release and GH mRNA expression in carp pituitary cells. Removal of endogenous GTH and GH by immunoneutralization with GTH and GH antisera, respectively, suppressed GH release, GH production, and GH mRNA levels. GH antiserum also blocked the stimulatory effects of exogenous GTH on GH release and GH mRNA levels. In reciprocal experiments, GH release and GH mRNA expression induced by exogenous GH was significantly reduced by GTH antiserum. In addition, exogenous GH was found to be inhibitory to basal GTH release and treatment with GH antiserum elevated GTH secretion at low doses but suppressed GTH production at high doses. These results suggest that local interactions between gonadotrophs and somatotrophs may form an intrapituitary feedback loop to regulate GH release and synthesis. In this model, GTH released from gonadotrophs induces GH release and GH production in neighboring somatotrophs. GH secreted maintains somatotroph sensitivity to GTH stimulation, and at the same time, inhibits basal GTH release in gonadotrophs. This feedback loop may represent a novel mechanism regulating GH release and synthesis in lower vertebrates.
Persistent Identifierhttp://hdl.handle.net/10722/179342
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Hen_US
dc.contributor.authorWang, Xen_US
dc.contributor.authorKo, WKWen_US
dc.contributor.authorWong, AOLen_US
dc.date.accessioned2012-12-19T09:54:19Z-
dc.date.available2012-12-19T09:54:19Z-
dc.date.issued2004en_US
dc.identifier.citationEndocrinology, 2004, v. 145 n. 12, p. 5548-5559en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/179342-
dc.description.abstractGonadotropin (GTH) and GH released from the pituitary are known to interact at multiple levels to modulate the functions of the gonadotrophic and somatotrophic axes. However, their interactions at the pituitary level have not been fully characterized. In this study, autocrine/paracrine regulation of GH synthesis and secretion by local interactions between gonadotrophs and somatotrophs was examined using grass carp pituitary cells as a cell model. Exogenous GTH and GH induced GH release and GH mRNA expression in carp pituitary cells. Removal of endogenous GTH and GH by immunoneutralization with GTH and GH antisera, respectively, suppressed GH release, GH production, and GH mRNA levels. GH antiserum also blocked the stimulatory effects of exogenous GTH on GH release and GH mRNA levels. In reciprocal experiments, GH release and GH mRNA expression induced by exogenous GH was significantly reduced by GTH antiserum. In addition, exogenous GH was found to be inhibitory to basal GTH release and treatment with GH antiserum elevated GTH secretion at low doses but suppressed GTH production at high doses. These results suggest that local interactions between gonadotrophs and somatotrophs may form an intrapituitary feedback loop to regulate GH release and synthesis. In this model, GTH released from gonadotrophs induces GH release and GH production in neighboring somatotrophs. GH secreted maintains somatotroph sensitivity to GTH stimulation, and at the same time, inhibits basal GTH release in gonadotrophs. This feedback loop may represent a novel mechanism regulating GH release and synthesis in lower vertebrates.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshAutocrine Communication - Physiologyen_US
dc.subject.meshCarps - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshGene Expression - Physiologyen_US
dc.subject.meshGonadotropins - Immunology - Metabolism - Pharmacologyen_US
dc.subject.meshGrowth Hormone - Genetics - Immunology - Secretionen_US
dc.subject.meshKineticsen_US
dc.subject.meshParacrine Communication - Physiologyen_US
dc.subject.meshPituitary Gland, Anterior - Cytology - Metabolism - Secretionen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.titleEvidence for a novel intrapituitary autocrine/paracrine feedback loop regulating growth hormone synthesis and secretion in grass carp pituitary cells by functional interactions between gonadotrophs and somatotrophsen_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.2004-0362en_US
dc.identifier.pmid15331572-
dc.identifier.scopuseid_2-s2.0-9444277946en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9444277946&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume145en_US
dc.identifier.issue12en_US
dc.identifier.spage5548en_US
dc.identifier.epage5559en_US
dc.identifier.isiWOS:000225109400019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhou, H=7404742310en_US
dc.identifier.scopusauthoridWang, X=8941883500en_US
dc.identifier.scopusauthoridKo, WKW=7202286890en_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US

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