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Article: Identification and characterization of the chicken galanin receptor GalR2 and a novel GalR2-like receptor (GalR2-L)

TitleIdentification and characterization of the chicken galanin receptor GalR2 and a novel GalR2-like receptor (GalR2-L)
Authors
KeywordsChicken
Galanin
Galanin Type Ii Receptor
Galanin-Like Peptide
Galr2-Like Receptor
Issue Date2012
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2012, v. 179 n. 2, p. 305-312 How to Cite?
AbstractIn mammals, the neuropeptide galanin exerts a variety of physiological roles in the neuroendocrine system through its interactions with three galanin receptor subtypes (GalR1, GalR2 and GalR3). However, little is known about the characteristics of galanin receptors in birds, and it is only recently that avian GalR1 and a novel GalR1-like receptor were first identified in chickens. In this study, we report the cDNA cloning and characterization of the other two chicken galanin receptors, the galanin type II receptor (cGalR2) and a novel GalR2-like receptor (GalR2-L), which share high degrees of similarity in sequence identity, gene structure and signaling properties. cGalR2 and cGalR2-L cDNAs encode two putative receptors of 371 and 370 amino acids, in which they show considerable amino acid sequence identities (65-67%, and 53-55%, respectively) with the mammalian GalR2. RT-PCR assays revealed that cGalR2 and cGalR2-L mRNA were widely expressed in the adult chicken tissues including the whole brain, intestine, lung, ovary, pituitary and different regions of the oviduct. As assayed with different luciferase reporter systems, chicken galanin (cGal 1-29) and human galanin-like peptide (hGALP 1-60) were demonstrated to stimulate the luciferase activities in Chinese hamster ovary cells expressing cGalR2 and cGalR2-L through the activations of cAMP/PKA, Ca 2+/calcineurin and MAPK/ERK signaling pathways, hence suggesting that both receptors are functionally coupled to the G s and G q proteins. Furthermore, the previously identified cGalR1 and cGalR1-L were found to be solely coupled to the G i/o proteins, and the hGALP (1-60) exhibited only a low potency to cGalR1, cGalR1-L, cGalR2 and cGalR2-L activations. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179315
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, JCWen_US
dc.contributor.authorJacobs, Ten_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:54:05Z-
dc.date.available2012-12-19T09:54:05Z-
dc.date.issued2012en_US
dc.identifier.citationGeneral And Comparative Endocrinology, 2012, v. 179 n. 2, p. 305-312en_US
dc.identifier.issn0016-6480en_US
dc.identifier.urihttp://hdl.handle.net/10722/179315-
dc.description.abstractIn mammals, the neuropeptide galanin exerts a variety of physiological roles in the neuroendocrine system through its interactions with three galanin receptor subtypes (GalR1, GalR2 and GalR3). However, little is known about the characteristics of galanin receptors in birds, and it is only recently that avian GalR1 and a novel GalR1-like receptor were first identified in chickens. In this study, we report the cDNA cloning and characterization of the other two chicken galanin receptors, the galanin type II receptor (cGalR2) and a novel GalR2-like receptor (GalR2-L), which share high degrees of similarity in sequence identity, gene structure and signaling properties. cGalR2 and cGalR2-L cDNAs encode two putative receptors of 371 and 370 amino acids, in which they show considerable amino acid sequence identities (65-67%, and 53-55%, respectively) with the mammalian GalR2. RT-PCR assays revealed that cGalR2 and cGalR2-L mRNA were widely expressed in the adult chicken tissues including the whole brain, intestine, lung, ovary, pituitary and different regions of the oviduct. As assayed with different luciferase reporter systems, chicken galanin (cGal 1-29) and human galanin-like peptide (hGALP 1-60) were demonstrated to stimulate the luciferase activities in Chinese hamster ovary cells expressing cGalR2 and cGalR2-L through the activations of cAMP/PKA, Ca 2+/calcineurin and MAPK/ERK signaling pathways, hence suggesting that both receptors are functionally coupled to the G s and G q proteins. Furthermore, the previously identified cGalR1 and cGalR1-L were found to be solely coupled to the G i/o proteins, and the hGALP (1-60) exhibited only a low potency to cGalR1, cGalR1-L, cGalR2 and cGalR2-L activations. © 2012 Elsevier Inc.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_US
dc.relation.ispartofGeneral and Comparative Endocrinologyen_US
dc.subjectChickenen_US
dc.subjectGalaninen_US
dc.subjectGalanin Type Ii Receptoren_US
dc.subjectGalanin-Like Peptideen_US
dc.subjectGalr2-Like Receptoren_US
dc.titleIdentification and characterization of the chicken galanin receptor GalR2 and a novel GalR2-like receptor (GalR2-L)en_US
dc.typeArticleen_US
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityWang, Y=rp00801en_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ygcen.2012.09.005en_US
dc.identifier.pmid22982974-
dc.identifier.scopuseid_2-s2.0-84866990737en_US
dc.identifier.hkuros212491-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84866990737&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume179en_US
dc.identifier.issue2en_US
dc.identifier.spage305en_US
dc.identifier.epage312en_US
dc.identifier.isiWOS:000310863500019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHo, JCW=37761650300en_US
dc.identifier.scopusauthoridJacobs, T=55373056100en_US
dc.identifier.scopusauthoridWang, Y=36062525200en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.citeulike11706569-

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