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Article: Molecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfields

TitleMolecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfields
Authors
KeywordsAlkane-Degrading Bacteria
Alkb Gene
Bioremediation
Denaturing Gradient Gel Electrophoresis (Dgge)
Oil Contamination
Issue Date2013
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration Biodegradation, 2013, v. 76, p. 49-57 How to Cite?
AbstractAlkane-degrading bacteria are crucial in the bioremediation of petroleum contamination from soil and groundwater. The alkane monooxygenase (alkB) gene encoding the Alk enzyme involved in aerobic degradation is a potentially functional gene biomarker for the detection of alkane-degrading bacteria. This study describes a denaturing gradient gel electrophoresis (DGGE) assay to facilitate the rapid and sensitive detection of alkB genes in wastewater from oilfield. The results showed that the presence of a considerable genetic diversity of alkB genes in the wastewater as evidenced by a total of 13 unique DNA bands detected. These alkB genes belong to nine genera, including Acinetobacter, Alcanivorax, Acidisphaera, Burkholderia, Geobacillus, Marinobacter, Mycobacterium, Pseudomonas, Rhodococcus and Xanthobacter. The abundance of alkane-degrading bacteria and total bacteria was calculated to range from 1.46 × 10 3 to 9.89 × 10 4 cell ml -1 and from 1.18 × 10 4 to 6.29 × 10 5 cells ml -1, respectively. The distribution of alkane-degrading bacteria was positively correlated with the environmental temperature and the specific types of n-alkanes in the wastewaters. Our results suggest that alkB-based DGGE methods could describe the diverse alkane-degrading bacteria in oil-degrading community, which may improve the understanding of the treatment for oil-polluted wastewater. © 2012.
Persistent Identifierhttp://hdl.handle.net/10722/179305
ISSN
2015 Impact Factor: 2.429
2015 SCImago Journal Rankings: 0.919
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, Hen_US
dc.contributor.authorWang, XLen_US
dc.contributor.authorMu, BZen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorLiu, YDen_US
dc.contributor.authorLin, KFen_US
dc.contributor.authorLu, SGen_US
dc.contributor.authorLu, Qen_US
dc.contributor.authorLi, BZen_US
dc.contributor.authorLi, YYen_US
dc.contributor.authorDu, XMen_US
dc.date.accessioned2012-12-19T09:54:00Z-
dc.date.available2012-12-19T09:54:00Z-
dc.date.issued2013en_US
dc.identifier.citationInternational Biodeterioration Biodegradation, 2013, v. 76, p. 49-57en_US
dc.identifier.issn0964-8305en_US
dc.identifier.urihttp://hdl.handle.net/10722/179305-
dc.description.abstractAlkane-degrading bacteria are crucial in the bioremediation of petroleum contamination from soil and groundwater. The alkane monooxygenase (alkB) gene encoding the Alk enzyme involved in aerobic degradation is a potentially functional gene biomarker for the detection of alkane-degrading bacteria. This study describes a denaturing gradient gel electrophoresis (DGGE) assay to facilitate the rapid and sensitive detection of alkB genes in wastewater from oilfield. The results showed that the presence of a considerable genetic diversity of alkB genes in the wastewater as evidenced by a total of 13 unique DNA bands detected. These alkB genes belong to nine genera, including Acinetobacter, Alcanivorax, Acidisphaera, Burkholderia, Geobacillus, Marinobacter, Mycobacterium, Pseudomonas, Rhodococcus and Xanthobacter. The abundance of alkane-degrading bacteria and total bacteria was calculated to range from 1.46 × 10 3 to 9.89 × 10 4 cell ml -1 and from 1.18 × 10 4 to 6.29 × 10 5 cells ml -1, respectively. The distribution of alkane-degrading bacteria was positively correlated with the environmental temperature and the specific types of n-alkanes in the wastewaters. Our results suggest that alkB-based DGGE methods could describe the diverse alkane-degrading bacteria in oil-degrading community, which may improve the understanding of the treatment for oil-polluted wastewater. © 2012.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_US
dc.relation.ispartofInternational Biodeterioration Biodegradationen_US
dc.subjectAlkane-Degrading Bacteriaen_US
dc.subjectAlkb Geneen_US
dc.subjectBioremediationen_US
dc.subjectDenaturing Gradient Gel Electrophoresis (Dgge)en_US
dc.subjectOil Contaminationen_US
dc.titleMolecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfieldsen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ibiod.2012.06.007en_US
dc.identifier.scopuseid_2-s2.0-84871808228en_US
dc.identifier.isiWOS:000312759300010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLi, H=55195467100en_US
dc.identifier.scopusauthoridWang, XL=55351929000en_US
dc.identifier.scopusauthoridMu, BZ=7004585949en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridLiu, YD=12785060300en_US
dc.identifier.scopusauthoridLin, KF=8595195600en_US
dc.identifier.scopusauthoridLu, SG=20734412800en_US
dc.identifier.scopusauthoridLu, Q=35513737300en_US
dc.identifier.scopusauthoridLi, BZ=35077122100en_US
dc.identifier.scopusauthoridLi, YY=27167834800en_US
dc.identifier.scopusauthoridDu, XM=36681308000en_US
dc.identifier.citeulike11574461-

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