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Article: Kisspeptin-1 directly stimulates LH and GH secretion from goldfish pituitary cells in a Ca 2+-dependent manner

TitleKisspeptin-1 directly stimulates LH and GH secretion from goldfish pituitary cells in a Ca 2+-dependent manner
Authors
KeywordsCell Column Perifusion Hormone Release
Fura-2 Ca 2+ Imaging
Gnrh-Stimulated Lh And Gh Release
Synthetic Goldfish Kisspeptin-1
Voltage-Sensitive Ca 2+ Channels
Issue Date2012
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2012, v. 179 n. 1, p. 38-46 How to Cite?
AbstractIt has been established that kisspeptin regulates reproduction via stimulation of hypothalamic gonadotropin-releasing hormone (GnRH) secretion, which then induces pituitary luteinizing hormone (LH) release. Kisspeptin also directly stimulates pituitary hormone release in some mammals. However, in goldfish, whether kisspeptin directly affects pituitary hormone release is controversial. In this study, synthetic goldfish kisspeptin-1 (1-10) (gKiss1) enhances LH and growth hormone (GH) release from primary cultures of goldfish pituitary cells in column perifusion. gKiss1 stimulation of LH and GH secretion were still manifested in the presence of the two native goldfish GnRHs, salmon (s)GnRH (goldfish GnRH-3) and chicken (c)GnRH-II (goldfish GnRH-2), but were attenuated by two voltage-sensitive calcium channel blockers, verapamil and nifedipine. gKiss-induced increases in intracellular Ca 2+ in Fura-2AM pre-loaded goldfish pars distalis cells were also inhibited by nifedipine. These results indicate that, in goldfish, (1) direct gKiss1 actions on pituitary LH and GH secretion exist, (2) these actions are independent of GnRH and (3) they involve Ca 2+ signalling. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179298
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChang, JPen_US
dc.contributor.authorMar, Aen_US
dc.contributor.authorWlasichuk, Men_US
dc.contributor.authorWong, AOLen_US
dc.date.accessioned2012-12-19T09:53:56Z-
dc.date.available2012-12-19T09:53:56Z-
dc.date.issued2012en_US
dc.identifier.citationGeneral And Comparative Endocrinology, 2012, v. 179 n. 1, p. 38-46en_US
dc.identifier.issn0016-6480en_US
dc.identifier.urihttp://hdl.handle.net/10722/179298-
dc.description.abstractIt has been established that kisspeptin regulates reproduction via stimulation of hypothalamic gonadotropin-releasing hormone (GnRH) secretion, which then induces pituitary luteinizing hormone (LH) release. Kisspeptin also directly stimulates pituitary hormone release in some mammals. However, in goldfish, whether kisspeptin directly affects pituitary hormone release is controversial. In this study, synthetic goldfish kisspeptin-1 (1-10) (gKiss1) enhances LH and growth hormone (GH) release from primary cultures of goldfish pituitary cells in column perifusion. gKiss1 stimulation of LH and GH secretion were still manifested in the presence of the two native goldfish GnRHs, salmon (s)GnRH (goldfish GnRH-3) and chicken (c)GnRH-II (goldfish GnRH-2), but were attenuated by two voltage-sensitive calcium channel blockers, verapamil and nifedipine. gKiss-induced increases in intracellular Ca 2+ in Fura-2AM pre-loaded goldfish pars distalis cells were also inhibited by nifedipine. These results indicate that, in goldfish, (1) direct gKiss1 actions on pituitary LH and GH secretion exist, (2) these actions are independent of GnRH and (3) they involve Ca 2+ signalling. © 2012 Elsevier Inc.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_US
dc.relation.ispartofGeneral and Comparative Endocrinologyen_US
dc.subjectCell Column Perifusion Hormone Releaseen_US
dc.subjectFura-2 Ca 2+ Imagingen_US
dc.subjectGnrh-Stimulated Lh And Gh Releaseen_US
dc.subjectSynthetic Goldfish Kisspeptin-1en_US
dc.subjectVoltage-Sensitive Ca 2+ Channelsen_US
dc.titleKisspeptin-1 directly stimulates LH and GH secretion from goldfish pituitary cells in a Ca 2+-dependent manneren_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ygcen.2012.07.028en_US
dc.identifier.pmid22885559-
dc.identifier.scopuseid_2-s2.0-84865089564en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84865089564&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume179en_US
dc.identifier.issue1en_US
dc.identifier.spage38en_US
dc.identifier.epage46en_US
dc.identifier.isiWOS:000309249700005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChang, JP=7601547649en_US
dc.identifier.scopusauthoridMar, A=55338014900en_US
dc.identifier.scopusauthoridWlasichuk, M=54796121100en_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US
dc.identifier.citeulike11502919-

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