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Article: Conversion of isoflavone glycoside to aglycones in soy protein isolate (SPI) using crude enzyme extracted from Bifidobacterium animalis Bb12 and Lactobacillus delbrueckii ssp. Bulgaricus ATCC 11842

TitleConversion of isoflavone glycoside to aglycones in soy protein isolate (SPI) using crude enzyme extracted from Bifidobacterium animalis Bb12 and Lactobacillus delbrueckii ssp. Bulgaricus ATCC 11842
Authors
KeywordsAglycones
Bifidobacterium
Biotransformation
Extracted Crude Enzyme
Glycosides
Isoflavones
Lactobacillus
Soy Protein Isolate
Issue Date2012
Citation
International Food Research Journal, 2012, v. 19 n. 2, p. 433-439 How to Cite?
AbstractCrude enzyme extracts from B. animalis Bb12 and L. delbrueckii ssp. bulgaricus ATCC 11842 were used at 0.1, 0.5, and 1.0 g/L to hydrolyse glycitin (isoflavone glycosides) to its biologically active form (isoflavone aglycones; IA) in soymilk (SM) prepared from soy protein isolate (SPI) supplemented with 2.0% (w/v) of D-glucose. Enumeration of microbial populations, measurement of pH and quantification of isoflavones was carried out at 0 h, 6 h and 12 h of fermentation. The quantification of isoflavone compounds in SM was carried out using HPLC. The biotransformation of glycitin was higher at the enzyme level of 1.0 g/L from B. animalis Bb12 at 12 h than that at 0.5 g/L or 0.1 g/L, and the level of biotransformation was 74.4%, while 75.23% of glycitin was biotransformed with the enzyme extracted from L. delbrueckii ssp. bulgaricus ATCC 11842 at the same level of enzyme. The decrease in pH by B. animalis Bb12 was lowest with 1.0 g/L and highest with the control (4.69). Similarly, the decrease in pH by L. delbrueckii ssp. bulgaricus ATCC 11842 was lowest with 1.0 g/L (5.19) and highest with the control (5.86). The final viable population of the B. animalis Bb12 ranged from 5.94 to 7.49 log CFU/mL and that of L. delbrueckii ssp. bulgaricus ATCC 11842 ranged from 4.42 to 6.70 log CFU/mL and the organisms showed the highest viable population of 6.70 log CFU/mL at 12 h with 1.0 g/L crude enzyme.
Persistent Identifierhttp://hdl.handle.net/10722/179269
ISSN
2015 SCImago Journal Rankings: 0.387
References

 

DC FieldValueLanguage
dc.contributor.authorPrasad, LNen_US
dc.contributor.authorShah, NPen_US
dc.date.accessioned2012-12-19T09:53:32Z-
dc.date.available2012-12-19T09:53:32Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Food Research Journal, 2012, v. 19 n. 2, p. 433-439en_US
dc.identifier.issn1985-4668en_US
dc.identifier.urihttp://hdl.handle.net/10722/179269-
dc.description.abstractCrude enzyme extracts from B. animalis Bb12 and L. delbrueckii ssp. bulgaricus ATCC 11842 were used at 0.1, 0.5, and 1.0 g/L to hydrolyse glycitin (isoflavone glycosides) to its biologically active form (isoflavone aglycones; IA) in soymilk (SM) prepared from soy protein isolate (SPI) supplemented with 2.0% (w/v) of D-glucose. Enumeration of microbial populations, measurement of pH and quantification of isoflavones was carried out at 0 h, 6 h and 12 h of fermentation. The quantification of isoflavone compounds in SM was carried out using HPLC. The biotransformation of glycitin was higher at the enzyme level of 1.0 g/L from B. animalis Bb12 at 12 h than that at 0.5 g/L or 0.1 g/L, and the level of biotransformation was 74.4%, while 75.23% of glycitin was biotransformed with the enzyme extracted from L. delbrueckii ssp. bulgaricus ATCC 11842 at the same level of enzyme. The decrease in pH by B. animalis Bb12 was lowest with 1.0 g/L and highest with the control (4.69). Similarly, the decrease in pH by L. delbrueckii ssp. bulgaricus ATCC 11842 was lowest with 1.0 g/L (5.19) and highest with the control (5.86). The final viable population of the B. animalis Bb12 ranged from 5.94 to 7.49 log CFU/mL and that of L. delbrueckii ssp. bulgaricus ATCC 11842 ranged from 4.42 to 6.70 log CFU/mL and the organisms showed the highest viable population of 6.70 log CFU/mL at 12 h with 1.0 g/L crude enzyme.en_US
dc.languageengen_US
dc.relation.ispartofInternational Food Research Journalen_US
dc.subjectAglyconesen_US
dc.subjectBifidobacteriumen_US
dc.subjectBiotransformationen_US
dc.subjectExtracted Crude Enzymeen_US
dc.subjectGlycosidesen_US
dc.subjectIsoflavonesen_US
dc.subjectLactobacillusen_US
dc.subjectSoy Protein Isolateen_US
dc.titleConversion of isoflavone glycoside to aglycones in soy protein isolate (SPI) using crude enzyme extracted from Bifidobacterium animalis Bb12 and Lactobacillus delbrueckii ssp. Bulgaricus ATCC 11842en_US
dc.typeArticleen_US
dc.identifier.emailShah, NP: npshah@hku.hken_US
dc.identifier.authorityShah, NP=rp01571en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-84857926673en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857926673&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume19en_US
dc.identifier.issue2en_US
dc.identifier.spage433en_US
dc.identifier.epage439en_US
dc.identifier.scopusauthoridPrasad, LN=55067579300en_US
dc.identifier.scopusauthoridShah, NP=7401823907en_US

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