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Article: Rapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryos

TitleRapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryos
Authors
Issue Date2011
PublisherCell Press. The Journal's web site is located at http://www.current-biology.com/
Citation
Current Biology, 2011, v. 21 n. 21, p. 1800-1807 How to Cite?
AbstractDNA injected into the Caenorhabditis elegans germline forms extrachromosomal arrays that segregate during cell division [1, 2]. The mechanisms underlying array formation and segregation are not known. Here, we show that extrachromosomal arrays form de novo centromeres at high frequency, providing unique access to a process that occurs with extremely low frequency in other systems [3-8]. De novo centromerized arrays recruit centromeric chromatin and kinetochore proteins and autonomously segregate on the spindle. Live imaging following DNA injection revealed that arrays form after oocyte fertilization via homologous recombination and nonhomologous end-joining. Individual arrays gradually transition from passive inheritance to active segregation during the early embryonic divisions. The heterochromatin protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for de novo centromerization even though arrays become strongly enriched for the heterochromatin-associated H3K9me3 modification over time. Partial inhibition of HP1 family proteins accelerates the acquisition of segregation competence. In addition to reporting the first direct visualization of new centromere formation in living cells, these findings reveal that naked DNA rapidly builds de novo centromeres in C. elegans embryos in an HP1-independent manner and suggest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centromerization. © 2011 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179259
ISSN
2015 Impact Factor: 8.983
2015 SCImago Journal Rankings: 4.729
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, KWYen_US
dc.contributor.authorNabeshima, Ken_US
dc.contributor.authorOegema, Ken_US
dc.contributor.authorDesai, Aen_US
dc.date.accessioned2012-12-19T09:53:26Z-
dc.date.available2012-12-19T09:53:26Z-
dc.date.issued2011en_US
dc.identifier.citationCurrent Biology, 2011, v. 21 n. 21, p. 1800-1807en_US
dc.identifier.issn0960-9822en_US
dc.identifier.urihttp://hdl.handle.net/10722/179259-
dc.description.abstractDNA injected into the Caenorhabditis elegans germline forms extrachromosomal arrays that segregate during cell division [1, 2]. The mechanisms underlying array formation and segregation are not known. Here, we show that extrachromosomal arrays form de novo centromeres at high frequency, providing unique access to a process that occurs with extremely low frequency in other systems [3-8]. De novo centromerized arrays recruit centromeric chromatin and kinetochore proteins and autonomously segregate on the spindle. Live imaging following DNA injection revealed that arrays form after oocyte fertilization via homologous recombination and nonhomologous end-joining. Individual arrays gradually transition from passive inheritance to active segregation during the early embryonic divisions. The heterochromatin protein 1 (HP1) family proteins HPL-1 and HPL-2 are dispensable for de novo centromerization even though arrays become strongly enriched for the heterochromatin-associated H3K9me3 modification over time. Partial inhibition of HP1 family proteins accelerates the acquisition of segregation competence. In addition to reporting the first direct visualization of new centromere formation in living cells, these findings reveal that naked DNA rapidly builds de novo centromeres in C. elegans embryos in an HP1-independent manner and suggest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centromerization. © 2011 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.current-biology.com/en_US
dc.relation.ispartofCurrent Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCaenorhabditis Elegans - Embryology - Genetics - Metabolismen_US
dc.subject.meshCaenorhabditis Elegans Proteins - Genetics - Metabolismen_US
dc.subject.meshCentromere - Metabolismen_US
dc.subject.meshChromosomal Proteins, Non-Histone - Genetics - Metabolismen_US
dc.subject.meshDna, Helminth - Metabolismen_US
dc.subject.meshHomologous Recombinationen_US
dc.subject.meshKinetochores - Metabolismen_US
dc.titleRapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryosen_US
dc.typeArticleen_US
dc.identifier.emailYuen, KWY: kwyyuen@hku.hken_US
dc.identifier.authorityYuen, KWY=rp01512en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.cub.2011.09.016en_US
dc.identifier.pmid22018540-
dc.identifier.scopuseid_2-s2.0-80755136621en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80755136621&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue21en_US
dc.identifier.spage1800en_US
dc.identifier.epage1807en_US
dc.identifier.isiWOS:000297139600017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYuen, KWY=8841935800en_US
dc.identifier.scopusauthoridNabeshima, K=7006395764en_US
dc.identifier.scopusauthoridOegema, K=6603708915en_US
dc.identifier.scopusauthoridDesai, A=7201793131en_US
dc.identifier.citeulike9951268-

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