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Article: Gonadotropins induce tumor cell migration and invasion by increasing cyclooxygenases expression and prostaglandin E2 production in human ovarian cancer cells

TitleGonadotropins induce tumor cell migration and invasion by increasing cyclooxygenases expression and prostaglandin E2 production in human ovarian cancer cells
Authors
Issue Date2010
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2010, v. 151 n. 7, p. 2985-2993 How to Cite?
AbstractGonadotropins (FSH and LH) are detectable in ovarian tumor fluid, suggesting a possible role for gonadotropins in ovarian carcinogenesis and progression. However, the molecular mechanisms behind the role of gonadotropins in ovarian cancer development are unknown. Cyclooxygenase (COX) enzymes, COX-1 and COX-2, play crucial roles in the pathogenesis of human malignancies. The purpose of the current study was to determine whether the effect of gonadotropins on ovarian cancer invasion is mediated by a COX-dependent mechanism. Here, we reported that FSH/LH can promote prostaglandin E 2 (PGE 2) production in ovarian cancer cells via COX-1 and -2 up-regulation at the protein and mRNA level. The phosphatidylinositol-3-kinase (PI3K)/AKT signaling pathway was required for gonadotropin-mediated up-regulation of COX-1 and COX-2. Moreover, treatmentwith COX-1- and COX-2-specific inhibitors abrogated the stimulatory effect of gonadotropins on motility and invasive activity. Western blot and gelatin zymography showed that COX-1 and COX-2 were critical for gonadotropin-induced expression of metastasis-related proteinases, matrix metalloproteinase (MMP)-2 and MMP-9. In addition, our results showed that PGE 2 induced an increase in cell invasiveness and the expression of MMP-2 and MMP-9 in ovarian cancer cells. These data show that COX-1 and COX-2 play essential roles in gonadotropin-induced migration and invasion. Copyright © 2010 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/179200
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, MTen_US
dc.contributor.authorWong, ASTen_US
dc.contributor.authorLeung, PCKen_US
dc.date.accessioned2012-12-19T09:52:48Z-
dc.date.available2012-12-19T09:52:48Z-
dc.date.issued2010en_US
dc.identifier.citationEndocrinology, 2010, v. 151 n. 7, p. 2985-2993en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/179200-
dc.description.abstractGonadotropins (FSH and LH) are detectable in ovarian tumor fluid, suggesting a possible role for gonadotropins in ovarian carcinogenesis and progression. However, the molecular mechanisms behind the role of gonadotropins in ovarian cancer development are unknown. Cyclooxygenase (COX) enzymes, COX-1 and COX-2, play crucial roles in the pathogenesis of human malignancies. The purpose of the current study was to determine whether the effect of gonadotropins on ovarian cancer invasion is mediated by a COX-dependent mechanism. Here, we reported that FSH/LH can promote prostaglandin E 2 (PGE 2) production in ovarian cancer cells via COX-1 and -2 up-regulation at the protein and mRNA level. The phosphatidylinositol-3-kinase (PI3K)/AKT signaling pathway was required for gonadotropin-mediated up-regulation of COX-1 and COX-2. Moreover, treatmentwith COX-1- and COX-2-specific inhibitors abrogated the stimulatory effect of gonadotropins on motility and invasive activity. Western blot and gelatin zymography showed that COX-1 and COX-2 were critical for gonadotropin-induced expression of metastasis-related proteinases, matrix metalloproteinase (MMP)-2 and MMP-9. In addition, our results showed that PGE 2 induced an increase in cell invasiveness and the expression of MMP-2 and MMP-9 in ovarian cancer cells. These data show that COX-1 and COX-2 play essential roles in gonadotropin-induced migration and invasion. Copyright © 2010 by The Endocrine Society.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.titleGonadotropins induce tumor cell migration and invasion by increasing cyclooxygenases expression and prostaglandin E2 production in human ovarian cancer cellsen_US
dc.typeArticleen_US
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_US
dc.identifier.authorityWong, AST=rp00805en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.2009-1318en_US
dc.identifier.pmid20392831-
dc.identifier.scopuseid_2-s2.0-77954569204en_US
dc.identifier.hkuros174119-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954569204&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume151en_US
dc.identifier.issue7en_US
dc.identifier.spage2985en_US
dc.identifier.epage2993en_US
dc.identifier.isiWOS:000279049300003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLau, MT=35082179700en_US
dc.identifier.scopusauthoridWong, AST=23987963300en_US
dc.identifier.scopusauthoridLeung, PCK=55085135300en_US

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