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Article: Enhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafine

TitleEnhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafine
Authors
KeywordsAurantiochytrium Mangrovei
Microalga
Squalene
Terbinafine
Issue Date2010
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0959-3993
Citation
World Journal Of Microbiology And Biotechnology, 2010, v. 26 n. 7, p. 1303-1309 How to Cite?
AbstractSqualene is an effective chemopreventive agent in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study is to increase the squalene content and yield in the thraustochytrid, Aurantiochytrium mangrovei FB3 through medium optimization and the treatment with terbinafine, an inhibitor of squalene monooxygenase in the sterol biosynthetic pathway. The highest biomass concentration of 21.2g l-1 was obtained at a glucose concentration of 60gl-1, while the highest specific growth rate of 0.077 h-1 and the growth yield coefficient of 0.44 gg-1 based on glucose were achieved at a lower glucose concentration (30g l-1). The addition of terbinafine led to a slight inhibition of cell growth whereas an obvious increase in squalene content was observed at terbinafine concentrations of 10 and 100mg l-1, which corresponded to an increase of 36 and 40% in squalene content, respectively compared to the control. The addition of terbinafine was thus effective in inducing the accumulation of squalene in A. mangrovei. This study not only demonstrated the production potential of squalene by A. mangrovei, but also provided novel information on the accumulation effect of terbinafine on the biosynthesis of an essential intermediate involved in sterol metabolic pathway. © Springer Science+Business Media B.V. 2010.
Persistent Identifierhttp://hdl.handle.net/10722/179196
ISSN
2015 Impact Factor: 1.532
2015 SCImago Journal Rankings: 0.634
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFan, KWen_US
dc.contributor.authorAki, Ten_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorJiang, Yen_US
dc.date.accessioned2012-12-19T09:52:46Z-
dc.date.available2012-12-19T09:52:46Z-
dc.date.issued2010en_US
dc.identifier.citationWorld Journal Of Microbiology And Biotechnology, 2010, v. 26 n. 7, p. 1303-1309en_US
dc.identifier.issn0959-3993en_US
dc.identifier.urihttp://hdl.handle.net/10722/179196-
dc.description.abstractSqualene is an effective chemopreventive agent in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study is to increase the squalene content and yield in the thraustochytrid, Aurantiochytrium mangrovei FB3 through medium optimization and the treatment with terbinafine, an inhibitor of squalene monooxygenase in the sterol biosynthetic pathway. The highest biomass concentration of 21.2g l-1 was obtained at a glucose concentration of 60gl-1, while the highest specific growth rate of 0.077 h-1 and the growth yield coefficient of 0.44 gg-1 based on glucose were achieved at a lower glucose concentration (30g l-1). The addition of terbinafine led to a slight inhibition of cell growth whereas an obvious increase in squalene content was observed at terbinafine concentrations of 10 and 100mg l-1, which corresponded to an increase of 36 and 40% in squalene content, respectively compared to the control. The addition of terbinafine was thus effective in inducing the accumulation of squalene in A. mangrovei. This study not only demonstrated the production potential of squalene by A. mangrovei, but also provided novel information on the accumulation effect of terbinafine on the biosynthesis of an essential intermediate involved in sterol metabolic pathway. © Springer Science+Business Media B.V. 2010.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0959-3993en_US
dc.relation.ispartofWorld Journal of Microbiology and Biotechnologyen_US
dc.subjectAurantiochytrium Mangroveien_US
dc.subjectMicroalgaen_US
dc.subjectSqualeneen_US
dc.subjectTerbinafineen_US
dc.titleEnhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafineen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11274-009-0301-2en_US
dc.identifier.pmid24026934-
dc.identifier.scopuseid_2-s2.0-77953693321en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953693321&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue7en_US
dc.identifier.spage1303en_US
dc.identifier.epage1309en_US
dc.identifier.isiWOS:000278695800018-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridFan, KW=7202978325en_US
dc.identifier.scopusauthoridAki, T=7006453167en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US
dc.identifier.citeulike6534821-

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