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Article: One amino acid substitution in phytoene desaturase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthin

TitleOne amino acid substitution in phytoene desaturase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthin
Authors
Issue Date2010
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2010, v. 232 n. 1, p. 61-67 How to Cite?
AbstractA stable Chlorella zofingiensis mutant (E17) produced by chemical mutagen was characterized with respect to growth, astaxanthin biosynthesis, and phytoene desaturation. The mutant E17 could grow well and produce normal levels of colored carotenoids in the presence of 0.25 μM norflurazon, in which the growth of wild type (WT) cells was greatly limited due to inhibited carotenoid formation. Induced by high-light irradiation or glucose, E17 produced 44 or 36% more astaxanthin than WT when cultured in media without norflurazon. A point mutation (C-T) was revealed to occur in the PDS gene of E17, leading to an amino acid change (L516F) in its coding region. The mutated PDS exhibited 31-fold resistance to norflurazon when compared to WT as determined by an in vitro assay. Surprisingly, the mutated PDS exhibited higher efficiency in converting phytoene to ζ-carotene. No difference in PDS transcripts was found between E17 and WT cells cultured either in normal or induced conditions. In contrast, higher transcript levels of β-carotene ketolase and hydroxylase were found in the E17 cells. Taken together, we conclude that a point mutation in Chlorella PDS gene makes E17 resistant to norflurazon and synthesize higher amounts of carotenoids including astaxanthin. © 2010 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/179189
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jen_US
dc.contributor.authorZhong, Yen_US
dc.contributor.authorSun, Zen_US
dc.contributor.authorHuang, Jen_US
dc.contributor.authorSandmann, Gen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:52:42Z-
dc.date.available2012-12-19T09:52:42Z-
dc.date.issued2010en_US
dc.identifier.citationPlanta, 2010, v. 232 n. 1, p. 61-67en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/179189-
dc.description.abstractA stable Chlorella zofingiensis mutant (E17) produced by chemical mutagen was characterized with respect to growth, astaxanthin biosynthesis, and phytoene desaturation. The mutant E17 could grow well and produce normal levels of colored carotenoids in the presence of 0.25 μM norflurazon, in which the growth of wild type (WT) cells was greatly limited due to inhibited carotenoid formation. Induced by high-light irradiation or glucose, E17 produced 44 or 36% more astaxanthin than WT when cultured in media without norflurazon. A point mutation (C-T) was revealed to occur in the PDS gene of E17, leading to an amino acid change (L516F) in its coding region. The mutated PDS exhibited 31-fold resistance to norflurazon when compared to WT as determined by an in vitro assay. Surprisingly, the mutated PDS exhibited higher efficiency in converting phytoene to ζ-carotene. No difference in PDS transcripts was found between E17 and WT cells cultured either in normal or induced conditions. In contrast, higher transcript levels of β-carotene ketolase and hydroxylase were found in the E17 cells. Taken together, we conclude that a point mutation in Chlorella PDS gene makes E17 resistant to norflurazon and synthesize higher amounts of carotenoids including astaxanthin. © 2010 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subject.meshAmino Acid Substitutionen_US
dc.subject.meshChlorella - Drug Effects - Enzymology - Growth & Developmenten_US
dc.subject.meshEscherichia Coli - Geneticsen_US
dc.subject.meshHerbicides - Pharmacologyen_US
dc.subject.meshMutationen_US
dc.subject.meshOxidoreductases - Chemistry - Geneticsen_US
dc.subject.meshPyridazines - Pharmacologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshXanthophylls - Biosynthesisen_US
dc.titleOne amino acid substitution in phytoene desaturase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthinen_US
dc.typeArticleen_US
dc.identifier.emailHuang, J: huangjc@hku.hken_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityHuang, J=rp00710en_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00425-010-1132-yen_US
dc.identifier.pmid20221629-
dc.identifier.scopuseid_2-s2.0-77952552490en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952552490&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume232en_US
dc.identifier.issue1en_US
dc.identifier.spage61en_US
dc.identifier.epage67en_US
dc.identifier.isiWOS:000277792700005-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLiu, J=36064082300en_US
dc.identifier.scopusauthoridZhong, Y=36098711000en_US
dc.identifier.scopusauthoridSun, Z=22837137300en_US
dc.identifier.scopusauthoridHuang, J=7408108735en_US
dc.identifier.scopusauthoridSandmann, G=7006654333en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.citeulike6854607-

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